The relative orientation of the lipid and carbohydrate moieties of lipochitooligosaccharides related to nodulation factors depends on lipid chain saturation

Groves, Patrick; Offermann, Stefanie; Rasmussen, Martin Ohsten; Cañada, F. Javier; Bono, Jean‐Jacques; Driguez, Hugues; Jiménez‐Barbero, Jesús

doi:10.1039/b500104h

Cited by 15 publications

(14 citation statements)

References 19 publications

(25 reference statements)

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“…[76] have an approximate molecular weight of 90 300, which can be converted to a molecular diffusion constant of D chitinase ¼ 58 mm 2 s 21 [77]. The product of chitinase activity, N-acetylglucosamine (GlcNAc), has a diffusion constant that is an order of magnitude larger: D GlcNAc ¼ 500 mm 2 s 21 [78]. We expect such a difference in the diffusion constants of extracellular enzymes and their digested products often to be upheld, because digestive enzymes are typically much larger than the nutrient molecules they release into the environment.…”

Section: Spatial Lineage Mixing and The Evolution Of Bacterial Coopermentioning

confidence: 99%

Cutting through the complexity of cell collectives

et al. 2013

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Via strength in numbers, groups of cells can influence their environments in ways that individual cells cannot. Large-scale structural patterns and collective functions underpinning virulence, tumour growth and bacterial biofilm formation are emergent properties of coupled physical and biological processes within cell groups. Owing to the abundance of factors influencing cell group behaviour, deriving general principles about them is a daunting challenge. We argue that combining mechanistic theory with theoretical ecology and evolution provides a key strategy for clarifying how cell groups form, how they change in composition over time, and how they interact with their environments. Here, we review concepts that are critical for dissecting the complexity of cell collectives, including dimensionless parameter groups, individual-based modelling and evolutionary theory. We then use this hybrid modelling approach to provide an example analysis of the evolution of cooperative enzyme secretion in bacterial biofilms.

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Section: Spatial Lineage Mixing and The Evolution Of Bacterial Coopermentioning

confidence: 99%

Cutting through the complexity of cell collectives

et al. 2013

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“…(Fig. 6) illustrates calibrations for proteins (6.5 -66 kDa) and oligosaccharides (0.2-1.4 kDa) [27,28]. A good correlation is observed for both series of standards.…”

Section: Molecular Weight Determinationmentioning

confidence: 51%

“…Molecular weight standards can facilitate molecular weight estimations and provide confidence in the quality of the instrument. Plot of log D versus log (molecular weight) for a series of oligosaccharides [28] (filled circles) and proteins [27] (open circles). The two fitted lines have a high correlation for the individual sets of data but clearly have different slopes.…”

Section: Molecular Weight Determinationmentioning

confidence: 99%

New Applications, Processing Methods and Pulse Sequences Using Diffusion NMR

Cobas¹,

Groves²,

Martín-Pastor³

et al. 2005

CAC

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Diffusion NMR, and in particular the DOSY processing method (Diffusion Ordered SpectroscopY), is an attractive technique to characterize mixtures without first having to separate the components. As a result, DOSY can yield a vast amount of analytical information. General applications of DOSY are reviewed here although we emphasize specialist applications that provide unique data. Such applications include the analysis of kinetic products, the detection of impurities in complex mixtures and the analysis of foodstuffs. We also focus on recent applications, such as the incorporation of DOSY into drug discovery protocols and as a filter in the analysis of natural product extracts or compound libraries. Depending on the characteristics of the sample under study, a careful choice of DOSY NMR experiment and its processing strategy is required to obtain optimum results. Moreover, this review describes the strengths and weakness of the different DOSY experimental and processing methods from the perspective of its application by the analytical chemist to a larger variety of sample types.

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“…Depending on the degree of saturation of the lipid chain, the relative orientation of the oligosaccharide and acyl moieties can range from being extended to essentially perpendicular to being quasi-parallel (Groves et al 2005;Morando et al 2011) and is likely involved in the specificity of perception (Gough and Cullimore 2011). LCO is lipophilic.…”

Section: Introductionmentioning

confidence: 98%

“…LCO is lipophilic. In aqueous solution, when the concentration is [10 -3 M, it forms aggregates, but it is likely monomeric at physiological concentrations (Groves et al 2005). In common vetch (Vicia sativa [L.]), LCO signals are accumulated with relatively low levels of uptake, and concentrated and immobilized by binding sites in the plant cell wall, which become saturated by micromolar concentrations, before interaction with any receptors, such as LYKs in the plasma membrane (Goedhart et al 2000).…”

Section: Introductionmentioning

confidence: 99%

The response of canola cultivars to lipo-chitooligosaccharide (Nod Bj V [C18:1, MeFuc]) and thuricin 17

et al. 2015

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Lipo-chitooligosaccharides (LCOs) are signal molecules that play a role in initiating the symbiotic legume-rhizobia and arbuscular mycorrhizal processes. Thuricin 17 is a bacteriocin produced by Bacillus thuringiensis strain NEB 17. Previous work has shown that the application of a 10 -6 M LCO solution, or a 10 -11 M thuricin 17 solution, can stimulate the germination and growth of a wide range of plants. The objectives were: to grow canola cultivar 04C111 under optimal temperature conditions in a greenhouse, and to assess the effect of LCO seed application and spray treatment on 04C111 floral initiation, architecture, and factors that contribute to yield; to assess the effect of a thuricin 17 seed application treatment upon factors that contribute to 04C111 yield in a stressfully high temperature greenhouse system; and to assess the effect of an LCO seed application and spray treatment on Topas floral initiation, architecture, and factors that contribute to yield, and seed quality, in a simulated ''cool spring'' greenhouse system. According to the 95 % confidence limits, Brassica napus cultivars grown from untreated seed produced 39 % fewer to 1 % fewer branches than plants grown from LCO-treated seed. Application of an LCO foliar spray increased the total of branch lengths per 04C111 plant by 16.93 ± 8.41 cm. Based on the stem-to-branch length ratio, LCO-sprayed 04C111 plants were 68 % less apically dominant than unsprayed plants. Topas plants that were sprayed with LCO produced seed with approximately 4.45 ± 0.23 % more oil (% d.w.) than unsprayed plants. For both cultivars, seed treatment with 10 -6 M of LCO produced plants with a higher average sum of primary, secondary, and tertiary branch (C1 cm) frequencies.

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The relative orientation of the lipid and carbohydrate moieties of lipochitooligosaccharides related to nodulation factors depends on lipid chain saturation

Cited by 15 publications

References 19 publications

Cutting through the complexity of cell collectives

Cutting through the complexity of cell collectives

New Applications, Processing Methods and Pulse Sequences Using Diffusion NMR

The response of canola cultivars to lipo-chitooligosaccharide (Nod Bj V [C18:1, MeFuc]) and thuricin 17

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