The relationships of genetic polymorphisms of the long noncoding RNA growth arrest-specific transcript 5 with uterine cervical cancer

Abstract

The purposes of the investigation were to examine the implications of long noncoding RNA growth arrest-specific transcript 5 (GAS5) in progression and clinicopathological factors of uterine cervical cancer, and patient survival in Taiwan. Genotypic distributions of two GAS5 genetic variants rs145204276 and rs55829688 were detected in 208 patients including 111 patients with invasive cancer, 97 with precancerous lesions as well as 307 control women using real-time polymerase chain reaction. It explored that patients with cervical precancerous lesion had lower rate of AGGCA deletion (Del) in both alleles (Del/Del) of GAS5 rs145204276 as compared with control women. Patients with invasive cancer did not exhibit higher rate of Del/Del. Meanwhile, there were no different genotypic distributions in rs55829688 among patients with cervical invasive cancer and those with precancerous lesions as well as control women. Moreover, cervical cancer patients with Ins (insertion, AGGCA)/Del and Del/Del (-/-) in GAS5 rs55829688 tended to have poorer hazard ratio (HR) of 5 years survival. In addition, lymph node metastasis status exerted the most significantly predictive of 5 years survival rate. Conclusively, GAS5 polymorphism rs145204276 is probably applicable to predict 5 years survival HR of cervical cancer patients. However, the mechanism elucidating the methylation status and transcription function of rs145204276 in uterine cervical cancer needs to be delineated for its unique implication in uterine cervical cancer.

Genetic Variants of lncRNA GAS5 Are Associated with the Clinicopathologic Development of Oral Cancer

“…In previous research, the GAS5 is associated with the progression of glioma [ 25 ]. Besides, the GAS5 SNP rs145204276 variant correlated with a poorer five-year survival rate in uterine cervical cancer [ 26 ]. According to the above evidences, the GAS5 could lead to tumor development, and SNP rs145204276 might be related to tumor progression [ 26 , 40 ], and the GAS5 can lead to proliferation and invasion of esophageal cancer, which is anatomically similar to the development site of oral cancer [ 41 ].…”

“…Besides, the GAS5 SNP rs145204276 variant correlated with a poorer five-year survival rate in uterine cervical cancer [ 26 ]. According to the above evidences, the GAS5 could lead to tumor development, and SNP rs145204276 might be related to tumor progression [ 26 , 40 ], and the GAS5 can lead to proliferation and invasion of esophageal cancer, which is anatomically similar to the development site of oral cancer [ 41 ]. On the other hand, the presence of GAS5 could suppress tumor invasion and proliferation via the miR-21/PTEN axis in the oral squamous cell carcinoma [ 42 ].…”

“…Two SNPs of GAS5 , meaning the rs145204276 (Ins/Del) and the rs55829688 (T/C), were chosen since their minor allele frequencies were more than 5 percent, and earlier studies indicated their influence in other cancers [ 26 , 27 ]. The genotyping procedure used in the current study was similar as a previous study [ 28 ]; the genome was firstly extracted from the leukocytes in the venous blood from each participant via the QIAamp DNA kits (Qiagen, Valencia, Valencia, CA, USA) according to the manufacturer’s instruction for DNA isolation [ 29 , 30 ].…”

The impact of Aurora kinase A genetic polymorphisms on cervical cancer progression and clinicopathologic characteristics

“…They were stored at 4 °C immediately. DNA was extracted using the QIAamp DNA Blood Mini Kit according to the manufacturer's instructions 28 , 29 . The recruited DNA was therefore dissolved in pH 7.8 TE buffer.…”

Tumor Suppressive Effects of GAS5 in Cancer Cells

“…According to data from the GTEX database, individuals with these variants had significantly higher GAS5 expression in esophageal mucosa tissues [25]. Weng et al revealed that cervical patients with allele deletion in the GAS5 rs145204276 are likely to have a poorer hazard ratio of 5 years survival [71]. In contrast, GAS5 rs145204276 allele deletion in cervical squamous cell carcinoma [26], urothelial cell carcinoma [24], and glioma [72] was associated with lower GAS5 expression and increased tumor risk.…”