The relationships between pea necrosis virus and bean yellow mosaic virus

Beczner, L.; Maat, D. Z.; Bos, L.

doi:10.1007/bf01976950

Cited by 14 publications

(16 citation statements)

References 5 publications

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“…Our present results largely agree with those of earlier reports [3,10] with the exception that isolate E197, earlier considered a necrotic strain of BYMV, now appears to be a CYV¥ strain according to inclusion-body type, host range, and size of coat protein. The present results also confirm the earlier grouping of the pea mosaic isolate E198 as a pea (yellow) mosaic strain of BYMV [9] characterized by yellow mosaic symptoms in pea and poor pathogenicity to Phaseolus-bean genotypes.…”

Section: Discussionsupporting

confidence: 95%

Identification of potyvirus isolates from faba bean (Vicia faba L.), and the relationships between bean yellow mosaic virus and clover yellow vein virus

Fortass¹,

Bos²,

Goldbach³

1991

Archives of Virology

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Clover yellow vein virus (CYVV) isolate H and the related potyvirus isolates E178, E197, and E242 could be distinguished from bean yellow mosaic (BYMV) isolates by their wider host range among non-legume test plant species, the peculiar enlargement of the nucleolus in infected plants, and the larger size of their coat protein as evidenced by slower migration in SDS-PAGE. Serologically, they are qualitatively indistinguishable in electro-blot immunoassay (EBIA) also with antibodies specific to the N-terminal part of BYMV-B25 coat protein, implying therefore that CYVV and BYMV coat proteins contain identical amino acid sequences in the N-terminal region. The faba-bean virus isolates from Sudan, Syria, and The Netherlands could be identified as BYMV isolates especially adapted to faba bean. All of them were weakly pathogenic to Phaseolus bean with the exception of SV205, assuming an intermediate position between Phaseolus-bean isolates, with low pathogenicity to faba bean, and faba-bean isolates, usually having low pathogenicity to Phaseolus bean. Strains of BYMV are thus hard to delimit.

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Section: Discussionsupporting

confidence: 95%

Identification of potyvirus isolates from faba bean (Vicia faba L.), and the relationships between bean yellow mosaic virus and clover yellow vein virus

Fortass¹,

Bos²,

Goldbach³

1991

Archives of Virology

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“…For instance, some workers regard BYMV and PMV to be strains of the same virus [4,7,20,39], while others consider them as distinct viruses [3,27]. Similarly, BYMV and CYVV are considered by some workers to be strains of the same virus [8,9,20], while others have suggested that they are distinct potyviruses [3,19,27].…”

Section: Discussionmentioning

confidence: 98%

Bean yellow mosaic, clover yellow vein, and pea mosaic are distinct potyviruses: evidence from coat protein gene sequences and molecular hybridization involving the 3? non-coding regions

Tracy

Frenkel

Gough

et al. 1992

Archives of Virology

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The sequences of the 3' 1019 nucleotides of the genome of an atypical strain of bean yellow mosaic virus (BYMV-S) and of the 3' 1018 nucleotides of the clover yellow vein virus (CYVV-B) genome have been determined. These sequences contain the complete coding region of the viral coat protein followed by a 3' non-coding region of 173 and 178 nucleotides for BYMV-S and CYVV-B, respectively. When the deduced amino acid sequences of the coat protein coding regions were compared, a sequence identity of 77% was found between the two viruses, and optimal alignment of the 3' untranslated regions of BYMV-S and CYVV-B gave a 65% identity. However, the degree of homology of the amino acid sequences of coat proteins of BYMV-S with the published sequences for three other strains of BYMV ranged from 88% to 94%, while the sequence homology of the 3' untranslated regions between the four strains of BYMV ranged between 86% and 95%. Amplified DNA probes corresponding to the 3' non-coding regions of BYMV-S and CYVV-B showed strong hybridization only with the strains of their respective viruses and not with strains of other potyviruses, including pea mosaic virus (PMV). The relatively low sequence identities between the BYMV-S and CYVV-B coat proteins and their 3' non-coding regions, together with the hybridization results, indicate that BYMV, CYVV, and PMV are distinct potyviruses.

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“…Viruses do not always fit neatly into taxonomic schemes, and due to the clonal rather than sexual nature of virus reproduction, the distinction between viruses and strains will always be arbitrary. The problem is especially common in the potyvirus and tymovirus groups (Bos, 1970;Beczner et al, 1976;Koenig, 1976;Lesemann et aL, 1979). As an aid in deciding whether a 'new' entity shall be considered as a new virus or a strain of an already established virus, we would like to endorse the scheme outlined in Table 4.…”

Section: Discussionmentioning

confidence: 99%