Guidelines for the Identification and Characterization of Plant Viruses

Particle Morphology and Cytopathology of Galinsoga Mosaic Virus

“…Particle morphology of viruses and their cytopathological effects are often used in virus identification and taxonomy (Hamilton et al, 1981). In this paper we report studies on these properties of GMV which were aimed at assigning the virus to the appropriate taxonomic group.…”

Production and use of monoclonal antibodies for molecular characterization, affinity purification, and serological detection of economically significant plant viruses

“…They are long flexuous rods measuring 680-900 X 11 nm (Fenner, 1976;Hamilton et al, 1981), their capsid consists of multiple copies of a single protein of molecular weight 23-36,000 (Hill and Benner, 1980a), and they contain a single stranded, positive-sense, RNA genome of molecular weight 2.3-3.5 X 10^ daltons (Hamilton et al, 1981;Hill and Benner, 1980b). The disease symptoms induced by SMV infection are varied (Koshimizu and lizuka, 1963;Walters, 1963), and are dependent upon cultivar, age of plants at the time of infection (Ross, 1969a), virus strain (Ross, 1969b(Ross, , 1975, and environmental factors such as temperature (Dunleavy et al, 1970;Ross, 1970;Porto and Hagedorn, 1975a), and light (Ross, 1970).…”

Production and use of monoclonal antibodies against the potato virus Y group

“…During infection, the potato virus Y group induces the formation of pinwheel inclusions in infected cells (?6, 40, 44, 103). These inclusions are unique to the potato virus Y group and are use ful for the diagnosis of infection at the group level (40,57). A further division of the group can also be made based on inclusion structure.…”

“…Their capsids consist of multiple copies of a single type of capsid protein having a molecular weight of 28-36,000 (57,61,64). A single stranded, positive (sense), RNA genome is present that has a molecular weight of 2.8-3.5 million daltons (57,62,64). During infection, the potato virus Y group induces the formation of pinwheel inclusions in infected cells (?6, 40, 44, 103).…”

“…Cell are long flexuous rods measuring 680-900 x 11 nm (44,57). Their capsids consist of multiple copies of a single type of capsid protein having a molecular weight of 28-36,000 (57,61,64).…”