The relationship of physical and chemical conditions of CEP diluent with egg yolk addition to bull spermatozoa quality before and after storage at temperaturof 4-5°C

Abstract

Abstract. Storage of semen requires diluent to dilute semen and maintain sperm quality. One of the diluent for bull semen was CEP. The purpose of this study was to assess the association of bull spermatozoa quality with the physical and chemical conditions of CEP diluents with the addition of egg yolk before and after the storage process. The study used Limousin bull with 5 replications. The quality of spermatozoa included motility and viability. Physical and chemical conditions included the pH and osmolarity of the diluent. The motility of spermatozoa was observed under a light microscope with 200 X magnification at 37°C by two people. The viability of spermatozoa was observed under a light microscope with 400 X magnification with nigrosine eosin staining. Data were analyzed with ANOVA and continued Duncan's test. Dilution pH was measured using pH indicator paper ranging from 6-8. The osmolarity of the diluent was measured by electrical osmolarity. The results showed that the addition of egg yolk in the CEP diluent decreased the pH and increased osmolartitas, but the quality of spermatozoa can be kept up to 8 days of storage. The conclusion in this study was the addition of egg yolk in the CEP diluent provided physical and chemical conditions that can maintain the quality of spermatozoa during storage at a temperature of 4-5 ° C.

Study of Soya Addition in Tris Base Extender on the Quality of Senduro Goat Spermatozoa and Membrane Integrity on Storage Temperature 4-5°C

“…Before AI is done, fresh semen that has been obtained then diluted using diluent media, and then carried out storage at low temperatures. Therefore dilution media are needed to maintain the quality of spermatozoa during the storing process [3,4,5].…”

“…So, it needs the addition of macromolecules [8,9] to protect sperm extracellularly during the dilution process. Macromolecules that are often added in semen diluent include egg yolks, skim milk, soya, albumin, and coconut milk [10,3]. The purpose of this study was to examine the effect of adding soya as macromolecules in the basic tris diluent on the quality and integrity of the Senduro Goat sperm membrane during 4-5 ° C storage temperature.…”