THE RELATIONSHIP OF pH OF THE GRANULATION TISSUE AND THE TAKE OF THE SKIN GRAFT

Ye, Richard C.

doi:10.1097/00006534-195703000-00004

Cited by 11 publications

(5 citation statements)

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“…Commercial, non-biomedical, calcium aluminate cements are used as sewer pipe lining due to their inherent acid resistance to biogenic corrosion. This acid-resistant feature may be useful in infection-laden sites of the human body where the pH value drops significantly [9]. In the biomedical field, these inert, biocompatible, and sufficiently strong cements have been successfully used as bone cements and in orthopedic implants [10].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Osteogenic/Dentinogenic Potential of an Experimental Calcium Aluminosilicate Cement

Eid

Niu

Primus

et al. 2013

Journal of Endodontics

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Introduction Calcium aluminosilicate cements are fast-setting, acid-resistant, bioactive cements that may be used as root-repair materials. This study examined the osteogenic/dentinogenic potential of an experimental calcium aluminosilicate cement (Quick-Set) using a murine odontoblast-like cell model. Methods Quick-Set and white ProRoot MTA (WMTA) were mixed with the proprietary gel or deionized water, allowed to set completely in 100% relative humidity and aged in complete growth medium for 2 weeks until rendered non-cytotoxic. Similarly-aged Teflon discs were used as negative control. The MDPC-23 cell-line was used for evaluating changes in mRNA expressions of genes associated with osteogenic/dentinogenic differentiation and mineralization (qRT-PCR) alkaline phosphatase enzyme production and extracellular matrix mineralization (Alizarin red-S staining). Results After MDPC-23 cells were incubated with the materials in osteogenic differentiation medium for 1 week, both cements showed upregulation in ALP and DSPP expression. Fold increases in these two genes were not significantly different between Quick-Set and WMTA. Both cements showed no statistically significant upregulation/downregulation in RUNX2, OCN, BSP and DMP1 gene expression compared with Teflon. Alkaline phosphatase activity of cells cultured on Quick-Set and WMTA were not significantly different at 1 week or 2 weeks, but were significantly higher (p<0.05) than Teflon in both weeks. Both cements showed significantly higher calcium deposition compared with Teflon after 3 weeks of incubation in mineralizing medium (p<0.001). Differences between Quick-Set and WMTA were not statistically significant. Conclusions The experimental calcium aluminosilicate cement exhibits similar osteogenic/dentinogenic properties to WMTA and may be a potential substitute for commercially-available tricalcium silicate cements.

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Section: Introductionmentioning

confidence: 99%

In Vitro Osteogenic/Dentinogenic Potential of an Experimental Calcium Aluminosilicate Cement

Eid

Niu

Primus

et al. 2013

Journal of Endodontics

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“…This is particularly important for the construction of capillaries in tissue-engineered organs. Furthermore, in a previous study by Ye (27), wherein the acceptance rate of skin grafts in patients with acute and chronic wounds of different origin was observed, 99% of the skin grafts were successfully taken in wounds at a pH of 7.4 and higher; however, no skin graft was successfully taken in wounds at a pH <7.0. In combination with the results of the present study, this may be due to the presence of appropriate growth conditions for capillaries at pH 7.4 and 7.6, which provide sufficient blood supply, leading to improved wound healing.…”

Section: Discussionmentioning

confidence: 90%

pH regulates the lumen diameter of tissue‑engineered capillaries

Wang

Bian

et al. 2022

Exp Ther Med

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Angiogenesis is vital in tissue engineering and the size of the capillary lumen diameter directly affects vascular function. Therefore, the involvement of the pH in the regulation of the capillary lumen diameter was investigated in the present study. The cytosolic pH of different pH medium groups was measured using flow cytometry. Bromodeoxyuridine staining and wound-healing assays were performed to detect cell proliferation and migration, respectively. The expression of angiogenesis-related genes was detected using reverse transcription-quantitative PCR. In addition, cell tube formation under different pH conditions was assessed using a tube formation assay and a 3D Matrigel ® model. The results indicated that a change in the pH value of the culture medium affected the cytosolic pH of the endothelial cells, which then led to a change in vascular diameter. When the medium's pH ranged from 7.4 to 7.6, the diameter of the lumen formed in the Matrigel was suitable for capillary formation in tissue engineering. The present results revealed an important role for the pH in the process of capillary formation and provided insight for pH regulation during endothelial cell tube formation and angiogenesis in tissue engineering.

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“…In 1956, Richard [89] described the take rate for 90 granulating wounds. In 12 cases transplanted at a pH of 6.8 to 7, the average take rate for the skin grafts was 0.1%; in 14 with a pH of 7.0 to 7.2, it was 6%; in 10 with pH 7.2 to 7.4, it was 47%.…”

Section: What Is the Optimal Take-rate Of Skin Grafts With Respect To The Ph?mentioning

confidence: 99%

Oxygen, pH, Lactate, and Metabolism—How Old Knowledge and New Insights Might Be Combined for New Wound Treatment

Haller¹,

Sander²,

Popp

et al. 2021

Medicina

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Over time, we have come to recognize a very complex network of physiological changes enabling wound healing. An immunological process enables the body to distinguish damaged cells and begin a cleaning mechanism by separating damaged proteins and cells with matrix metalloproteinases, a complement reaction, and free radicals. A wide variety of cell functions help to rebuild new tissue, dependent on energy provision and oxygen supply. Like in an optimized “bio-reactor,” disturbance can lead to prolonged healing. One of the earliest investigated local factors is the pH of wounds, studied in close relation to the local perfusion, oxygen tension, and lactate concentration. Granulation tissue with the wrong pH can hinder fibroblast and keratinocyte division and proliferation, as well as skin graft takes. Methods for influencing the pH have been tested, such as occlusion and acidification by the topical application of acidic media. In most trials, this has not changed the wound’s pH to an acidic one, but it has reduced the strong alkalinity of deeper or chronic wounds. Energy provision is essential for all repair processes. New insights into the metabolism of cells have changed the definition of lactate from a waste product to an indispensable energy provider in normoxic and hypoxic conditions. Neovascularization depends on oxygen provision and lactate, signaling hypoxic conditions even under normoxic conditions. An appropriate pH is necessary for successful skin grafting; hypoxia can change the pH of wounds. This review describes the close interconnections between the local lactate levels, metabolism, healing mechanisms, and pH. Furthermore, it analyzes and evaluates the different possible ways to support metabolism, such as lactate enhancement and pH adjustment. The aim of wound treatment must be the optimization of all these components. Therefore, the role of lactate and its influence on wound healing in acute and chronic wounds will be assessed.

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THE RELATIONSHIP OF pH OF THE GRANULATION TISSUE AND THE TAKE OF THE SKIN GRAFT

Cited by 11 publications

References 0 publications

In Vitro Osteogenic/Dentinogenic Potential of an Experimental Calcium Aluminosilicate Cement

In Vitro Osteogenic/Dentinogenic Potential of an Experimental Calcium Aluminosilicate Cement

pH regulates the lumen diameter of tissue‑engineered capillaries

Oxygen, pH, Lactate, and Metabolism—How Old Knowledge and New Insights Might Be Combined for New Wound Treatment

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