In Vitro Osteogenic/Dentinogenic Potential of an Experimental Calcium Aluminosilicate Cement

Abstract

Introduction Calcium aluminosilicate cements are fast-setting, acid-resistant, bioactive cements that may be used as root-repair materials. This study examined the osteogenic/dentinogenic potential of an experimental calcium aluminosilicate cement (Quick-Set) using a murine odontoblast-like cell model. Methods Quick-Set and white ProRoot MTA (WMTA) were mixed with the proprietary gel or deionized water, allowed to set completely in 100% relative humidity and aged in complete growth medium for 2 weeks until rendered non-cytotoxic. Similarly-aged Teflon discs were used as negative control. The MDPC-23 cell-line was used for evaluating changes in mRNA expressions of genes associated with osteogenic/dentinogenic differentiation and mineralization (qRT-PCR) alkaline phosphatase enzyme production and extracellular matrix mineralization (Alizarin red-S staining). Results After MDPC-23 cells were incubated with the materials in osteogenic differentiation medium for 1 week, both cements showed upregulation in ALP and DSPP expression. Fold increases in these two genes were not significantly different between Quick-Set and WMTA. Both cements showed no statistically significant upregulation/downregulation in RUNX2, OCN, BSP and DMP1 gene expression compared with Teflon. Alkaline phosphatase activity of cells cultured on Quick-Set and WMTA were not significantly different at 1 week or 2 weeks, but were significantly higher (p<0.05) than Teflon in both weeks. Both cements showed significantly higher calcium deposition compared with Teflon after 3 weeks of incubation in mineralizing medium (p<0.001). Differences between Quick-Set and WMTA were not statistically significant. Conclusions The experimental calcium aluminosilicate cement exhibits similar osteogenic/dentinogenic properties to WMTA and may be a potential substitute for commercially-available tricalcium silicate cements.

Study on Biocompatibility and Mineralization Potential of Capseal

“…For this reason, the upregulation of ALP, OCN, and BSP could suggest the osteogenic potential of certain dental materials. The upregulation of ALP by mineral trioxide aggregate was reported in many previous studies using rat dental papilla 21) , rat dental pulp 22) , and MDPC-23 cells 23) . BSP upregulation by mineral trioxide aggregate was also cited in previous studies using cementoblast 24) and dental pulp cells 25) .…”

Histologic Assessment of Quick-Set and Mineral Trioxide Aggregate Pulpotomies in a Canine Model

“…More recently, the cytotoxicity and osteogenic properties of WMTA and QS were compared using murine odontoblastlike cells. These in vitro studies showed that QS and WMTA had similar cytotoxic and osteogenic/dentinogenic properties (12,13).…”

Effects of tricalcium silicate cements on osteogenic differentiation of human bone marrow-derived mesenchymal stem cells in vitro

“…Previous studies have reported that TSCs increase the osteogenic potential of osteoblast precursors [33], pre-odontoblasts [34], human osteosarcoma cells [3], periodontal ligament and gingival fibroblast cells [4], osteoblasts [5], osteoblast precursor cells [9], dental pulp stem cells [10], human tooth germ stem cells [35]. To our knowledge, this is the first study that examined the effects of TSCs on the osteoactivity of a pleuripotent, human adult MSC cell line derived from the bone marrow.…”