The Relationship of Oxygen Uptake to Superoxide Dismutase and Catalase Activity in Human Skeletal Muscle

Abstract

A considerable amount of evidence now makes it clear that aerobic tissues require an elaborate enzyme system to remove the harmful reaction products of oxygen reduction. A portion of this protective system has been studied in human muscle and rat tissues. The VO2 max as well as the superoxide dismutase and catalase activity of vastus lateralis muscle of 12 healthy, male subjects was measured. The subjects with a high aerobic capacity (VO2 max greater than 60 ml . kg-1 . min-1) had significantly greater levels of both superoxide dismutase and catalase. There was also a linear relationship between both superoxide dismutase and catalase and VO2 max. The tissue oxygen consumption, and enzyme activity of the liver, heart, lung, and gastrocnemius from 24 rats was also studied. There VO2 and tissue enzyme activity of both superoxide dismutase and catalase.

Response of Sod-2 enzyme activity to selection for high voluntary wheel running

“…For example, superoxide dismutase (Sod) and catalase are enzymes which eliminate O − 2 and H 2 O 2 respectively, and consequently reduce the amount of oxidative damage that occurs (Weindruch et al, 1993). Some evidence supports the idea that longterm exercise training may also increase the level of antioxidant enzyme activity, as well as generally improving Heredity the health of an individual while aging (Jenkins et al, 1984;Ji et al, 1986Ji et al, , 1990Ji et al, , 1991. Indeed, much evidence now supports oxidative damage as a probable proximate mechanism of senescence in mammals and insects (Sohal and Orr, 1992;Agarwal and Sohal, 1993;Barja et al, 1994;Orr and Sohal, 1994;Sohal et al, 1995;Sohal and Weindruch, 1996;Beckman and Ames, 1998).…”

Skeletal muscle dysfunction and oxidative stress in patients with chronic obstructive lung disease

“…Consequently, pulmonary rehabilitation is recommended as an integral part of the management of COPD patients 74) . In general, physical exercise training can increase the antioxidant and oxidative capacity in skeletal muscle of healthy subjects 21,41,75,76) . Exercise training has also been reported to improve skeletal muscle oxidative capacity in patients with moderate to severe COPD 77) .…”

Physical exercise as pro-oxidant

“…Com relação a SOD, observa-se um a m aior consistência dos resultados obtidos, porém, ainda com alguma contradição (Jenkins, 1988;Quiroga, 1992). Jenkins et alii (1984), K anter et alii (1985), Q uintanilha (1984), relataram que a SOD m uscular total apresenta atividade aum entada pelo treinam ento de resistência. Entretanto, Laughlin et alii (1990) não encontraram aum ento da sua atividade no músculo esquelético com este tipo de treinam ento físico.…”

“…Contudo, relataram incremento em 37% na atividade da M n-SOD (SOD m itocondrial) nas fibras verm elhas de contração rápida (tipo lia) e nas fibras verm elhas de contração lenta (tipo I) e, de apenas 14% nas fibras musculares brancas (tipo Ilb) nesta condição. Jenkins et alii (1984), além de avaliar a atividade da SOD total e catalase no músculo vasto lateral de humanos, observaram que havia nítida correlação entre as atividades destas enzimas com o consumo de oxigênio. Contudo, resultados controversos entre a relação do consumo de oxigênio e atividade das enzimas antioxidantes foram obtidos por O hno et alii (1988).…”

“…Higuchi et alii (1985) também não encontraram aumento da sua atividade após três meses de treinam ento de resistência. Por outro lado, Jenkins et alii (1984Jenkins et alii ( ), K anter et alii (1985Jenkins et alii ( ), Q uintanilha (1984 obtiveram elevação da atividade da catalase no músculo esquelético com o treinam ento físico de resistência. Laughlin et alii (1990).…”