The relationship between renal warm ischemia time and glomerular loss. An experimental study in a pig model

Damasceno-Ferreira, José Aurelino; Bechara, Gustavo Ruschi; Costa, Waldemar S.; Pereira‐Sampaio, Marco A.; Sampaio, Francisco J.B.; Souza, Diogo Benchimol de

doi:10.1590/s0102-865020170050000002

Cited by 14 publications

(15 citation statements)

References 28 publications

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“…The right kidneys of all animals (organs not submitted to any procedure) were collected and fixed in 4% buffered formaldehyde. Kidneys were weighed and their volumes were estimated by the Scherle’s method [10]. The cortical-medullar ratio was estimated using Cavalieri’s principle [11, 12], and the absolute cortical volume (CV) was calculated by multiplying the cortical-medullary ratio by the renal volume.…”

Section: Methodsmentioning

confidence: 99%

Does a Ureteral Obstruction Affect the Contralateral Kidney Morphology? A Stereological Analysis in a Rodent Model

et al. 2018

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Objective: To investigate, in a rodent model, the influence of an obstructed kidney/ureter on the contralateral organ morphology. Methods: Thirty-six rats were randomly assigned into the following groups: untreated ureteral obstruction group (UO-U, n = 10), submitted to a ligature of the ureter at day 0; UO group (at day 0) followed by nephrectomy after 24 h (UO-N, n = 8); nephrectomy group (N, n = 9), submitted to nephrectomy at day 0; and sham group (S, n = 9), submitted to simulated surgery at day 0. All these procedures were performed on the left kidney/ureter. All animals were euthanized 30 days after surgery, and the right kidneys were collected for stereological analysis. Data were compared using a one-way analysis of variance with Bonferroni’s post test, considering p < 0.05 as significant. Results: The kidney volume, weight, and cortical volume were augmented in groups UO-N and N, compared to group S. Kidneys from the UO-U group showed only a moderate augmentation in kidney weight, with a reduction in the cortical-medullar ratio, compared to the kidneys in group S. No differences in the glomerular volumetric density, volume-weighted glomerular volume, and number of glomeruli were observed among the right kidneys of the studied groups. Conclusion: An obstructed kidney/ureter is more prejudicial to the contralateral kidney morphology than an absent kidney.

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Section: Methodsmentioning

confidence: 99%

Does a Ureteral Obstruction Affect the Contralateral Kidney Morphology? A Stereological Analysis in a Rodent Model

et al. 2018

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“…Kidneys were weighed and their volumes were estimated by the Scherle's method [13]. Left kidneys were transversely sliced for sequential sections of 2 mm thickness, which were used for determining the cortical-medullar ratio using Cavalieri's principle [14,15].…”

Section: Biometrical and Morphometrical Analysesmentioning

confidence: 99%

Immediate and Late Effects of Stress on Kidneys of Prepubertal and Adult Rats

Marchon

Ribeiro

Costa

et al. 2018

Kidney Blood Press Res

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Background/Aims: Stress had been related to physiological and morphological alterations in different organs, however kidney morphology and function after stress conditions are not well-known. To evaluate the effects of chronic stress on renal morphology in prepubertal and adult rats, and to investigate if the effects of induced stress remain stable or worsen following removal of the stress stimuli. Methods: Forty prepubertal rats (4 weeks old) and thirty six adult rats (10 weeks old) were used. Each age group was divided a into control group and a stressed group. Stressed animals were restrained in rigid tubes 2 hours per day for 6 weeks. Animals were euthanized 24 hours after the last stress stimulus for immediate evaluation (50% of the groups), and 6 weeks after the last stress stimulus for late evaluation (50% of the groups). Kidney weight and volume, cortical-medullar ratio, glomerular volumetric density, volume-weighted glomerular volume, and number of glomeruli per kidney were evaluated using the Image J software. Results: Prepubertal stressed animals showed reduced kidney weight and volume, and increased cortical-medullar ratio in comparison to its control group when evaluated immediately. Furthermore, stressed prepubertal and adult animals evaluated immediately had reduced glomerular volumetric density. Most importantly, all stressed groups exhibited reduced number of glomeruli per kidney. Other analyzed parameters did not difeer significantly the groups. Conclusion: Chronic stress induced before and after puberty led to irreversible glomerular loss, however, renal impairment was interrupted by removal of the stress stimuli.

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“…On the other hand, in nephrectomies performed at 1.0cm and 1.5cm cranially to the hilum, there was no lesion in the cranial division of the renal artery and the arterial supply to the midzone of the kidney was preserved as in humans (18). Thus, although the pig kidney does not have a retropielic artery, it remains the best animal model for partial upper pole nephrectomy when vascular injury is an important point for the study, and it is still the most used model in partial nephrectomies (22)(23)(24).…”

Section: Discussionmentioning

confidence: 99%

Anatomical background of ovine kidney for use as animal model: analysis of arterial segmentation, proportional volume of each segment and arterial injury after cranial pole partial nephrectomy

et al. 2020

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Objective: To study the arterial segments of ovine kidney, present a proportional volume analysis of each kidney arterial segment, and analyze arterial injuries caused by simulated partial nephrectomy of cranial pole. Materials and Methods: Forty-eight ovine kidneys injected with polyester resin into the renal arteries and collecting system were used in this study. Eighteen kidneys were used to study the arterial segments and the proportional volume of each renal segment. Other 30 kidneys were submitted to superior pole resection at a distance of 1.0cm, 0.5cm, or exactly at the cranial hilar edge, just before the resin hardening. These endocasts were used to evaluate the arterial injuries caused by these different resection planes. Results: Ovine renal artery divided into two (ventral and dorsal) or three segmental arteries. Dorsal segment presented higher proportional volume than ventral segment. For kidneys with three segments, the third segment was on the caudal region (caudoventral or caudo-dorsal segment) and presented the lowest proportional volume. None of the resected kidneys (at 1.0, 0.5 or at the cranial hilar edge) presented injury of arterial branches that irrigate non-resected region. Conclusion: The segmental distribution of renal artery, the proportional volume of each segment and arterial injuries after cranial pole resection in ovine kidneys are different from what is observed in human kidneys. Meanwhile, ovine kidneys show a primary segmental division on anterior and posterior, as in humans, but different from swine. These anatomical characteristics should be considered when using ovine as animal models for renal experimental and/or training procedures.

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The relationship between renal warm ischemia time and glomerular loss. An experimental study in a pig model

Cited by 14 publications

References 28 publications

Does a Ureteral Obstruction Affect the Contralateral Kidney Morphology? A Stereological Analysis in a Rodent Model

Does a Ureteral Obstruction Affect the Contralateral Kidney Morphology? A Stereological Analysis in a Rodent Model

Immediate and Late Effects of Stress on Kidneys of Prepubertal and Adult Rats

Anatomical background of ovine kidney for use as animal model: analysis of arterial segmentation, proportional volume of each segment and arterial injury after cranial pole partial nephrectomy

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