The relationship between RAPD markers and quantitative traits of caraway (Carum carvi L.)

Bocianowski, Jan; Seidler-Łożykowska, K.

doi:10.1016/j.indcrop.2011.08.019

Cited by 21 publications

(16 citation statements)

References 16 publications

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“…Breeders have made crosses between selected but restricted number of double low rapeseed genotypes within the breeding gene pool. Today, molecular restriction fragment length polymorphism, RAPD, AFLP, single sequence repeat, and single-nucleotide polymorphism markers have been extensively used for the estimation of genetic distance or genetic similarity and provide more information than is available from pedigree information (Irzykowska and Bocianowski, 2008;Krzymińska et al, 2008;Wolko et al, 2010;Bus et al, 2011;Bocianowski and Seidler-Łożykowska, 2012;Irzykowska et al, 2012Irzykowska et al, , 2013Seidler-Łożykowska et al, 2014;Varghese et al, 2014;Abbasi et al, 2015). The objective of this study was to characterize the levels and patterns of genetic similarity among the 101 B. napus genotypes of different origin using selected AFLP primer combinations.…”

Section: Discussionmentioning

confidence: 99%

Assessment of Genetic Relationships in Breeding Lines and Cultivars of Brassica napus and Their Implications for Breeding Winter Oilseed Rape

Liersch

Bocianowski

Wos³

et al. 2016

Crop Science

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The level of genetic diversity or similarity between parents has been proposed as a predictor of F1 generation performance and heterosis for seed yield. The objective of this study was to determine the feasibility of using amplified fragment length polymorphism (AFLP) fluorescent‐labeled primers to evaluate the genetic relationships between 101 oilseed rape (Brassica napus L.) genotypes to maximize the selection of different parents in a breeding program. The collection of genotypes included 25 cytoplasmic male sterility ogura and 21 restorer lines (Rfo). The set of genotypes was completed with B. napus cultivars of Polish, European, and Asian origin and resynthesized lines. Ten primer‐enzyme combinations (EcoRI and MseI) were used. Genetic similarities among genotypes were estimated in accordance with the Nei and Li coefficient of similarity, and then the unweighted pair group method using arithmetic averages method was applied for the creation of a dendrogram. A total of 344 reproducible, polymorphic markers were obtained. Unweighted pair group method using arithmetic averages clustering based on the Nei and Li coefficient of similarity separated the 101 genotypes into four distinct clusters. Analysis of molecular variance showed that 90% of the detected variation was found within the group, while the variation among groups contributed to only 10%. Our results indicate that the AFLP technique seemed to be a suitable molecular method for assessing genetic relationships between oilseed rape inbred lines with high levels of accuracy. Genetic similarity evaluated on the basis of AFLP molecular markers is a significant addition to classical methods of selection of parental components of winter oilseed rape hybrid varieties (e.g., ‘Poznaniak’).

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Section: Discussionmentioning

confidence: 99%

Assessment of Genetic Relationships in Breeding Lines and Cultivars of Brassica napus and Their Implications for Breeding Winter Oilseed Rape

Liersch

Bocianowski

Wos³

et al. 2016

Crop Science

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“…In yet another study (Kwon et al 2013), genetic diversity, population structure and genome-wide marker-trait association analyses of 298 homozygous lettuce (Lactuca sativa L.) lines identified nine single nucleotide polymorphic (SNP) markers which were significantly (p \ 0.0001) associated with phenotypic trait. Bocianowskia and Katarzyna (2012) studied the relationships between randomly amplified polymorphic DNA (RAPD) markers and 22 quantitative traits of caraway (Carum carvi L.) using regression analysis and found 44 RAPD markers to be associated with at least one trait. Similarly, several markers which have been identified to be associated with valerenic acid content and antioxidant activity were found to show an interactive effect of selected independent variables on the dependent variable (Steel and Torrie 1980;Pradeep et al 2007).…”

Section: Association Of Issr Markers With Biochemical Traitsmentioning

confidence: 99%

Identification of ISSR markers associated with valerenic acid content and antioxidant activity in Valeriana jatamansi Jones in the West Himalaya

2015

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Valeriana jatamansi Jones, commonly known as Indian Valerian or Tagar (Family: Valerinaceae) is used in both traditional and modern systems of medicine. The plants of this species are known for their high content of valerenic acid, the main active constituent of valerian, and high antioxidant activity, and these characteristics have increased the demand for these plants by the pharmaceutical industry. At present, the demand for planting material is largely met from the harvesting of wild plants, which vary in quality and quantity of the active ingredient. Therefore, there is a need to identify individual plants/populations with a higher content of the active ingredients and higher antioxidant activity. We used inter-simple sequence repeats (ISSR) markers in 151 genotypes of 25 V. jatamansi populations to identify markers associated with valerenic acid and antioxidant activity. Of the 130 ISSR primers tested, 20 generated 159 bands, with an average of 7.95 bands per primer. Valerenic acid content was significantly (p \ 0.05) higher in the Katarmal (aerial portion 0.57 ± 0.04 %) and Joshimath populations (root portion 1.80 ± 0.12 %). Antioxidant activity using these different in vitro assays varied among the populations and plant portions, with maximum antioxidant activity found in the aerial plant portion (8.63 ± 0.06 mM) and roots [8.36 ± 0.0 mM ascorbic acid equivalents (AAE)/100 g dry weight (dw)] of the Didihat and Katarmal populations, respectively, using the ABTS [2, 2 0 -azino-bis(3-ethylbenzothiazoline-6-sulphonic)] assay. The DPPH (2, 2-diphenyl-1-picryylhydrazyl free radical scavenging) assay revealed maximum antioxidant activity in the aerial plant portion (15.23 ± 0.09 mM) and roots (17.53 ± 0.04 mM AAE/100 g dw) of the Didihat population. The FRAP (ferric-reducing antioxidant power) assay showed that the roots of plants of the Ukhimath population had significantly higher antioxidant activity (12.71 ± 0.04 mM AAE/100 g dw) than those of other populations of V. jatamansi. Based on the stepwise multiple regression analysis, seven positive and six negative markers showed significant association with valerenic acid content. Antioxidant activity measured by the ABTS, DPPH and FRAP assays showed a positive correlation with 14, 13 and 10 markers, respectively (p \ 0.001). These markers have the potential for application in breeding programmes in order to select lineages of V. jatamansi with higher biochemical attributes, especially when no other genetic information, such as linkage maps and quantitative trait loci is available.Electronic supplementary material The online version of this article (

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“…Most of the MTAs were different under the two irrigation regimes, indicating the environmental effects in these associations [51]. These results showed that different genes may contribute to the same trait in different environments [52] or there might be a change in the expression level of the same gene between the two environments [48].…”

Section: Discussionmentioning

confidence: 77%

Marker-trait association analysis for drought tolerance in smooth bromegrass

Saeidnia

Majidi

Mirlohi

2020

Preprint

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Background: Little information is available on the application of marker-trait association (MTA) analysis for traits related to drought tolerance in smooth bromegrass. The objectives of this study were to identify marker loci associated with important agronomic traits and drought tolerance indices as well as fining stable associations in a diverse panel of polycross derived genotypes of smooth bromegrass. Phenotypic evaluations were performed at two water environments (normal and drought stress) during two years; and association analysis was done with 626 SRAP markers. Results: The results of population structure analysis identified three main subpopulations possessing significant genetic differences. Under normal irrigation, 68 and 57 marker-trait associations were identified using general linear model (GLM) and mixed linear mode1 (MLM), respectively. While under deficit irrigation, 61 and 54 markers were associated with the genes controlling the studied traits, based on these two models, respectively. Some of the markers were associated with more than one trait. It was revealed that markers Me1/Em5-11, Me1/Em3-15, and Me5/Em4-7 were consistently linked with drought-tolerance indices. Conclusion: Following marker validation, the MTAs reported in this panel could be useful tools to initiate marker-assisted selection (MAS) and targeted trait introgression of smooth bromegrass under normal and deficit irrigation regimes, and possibly fine mapping and cloning of the underlying genes and QTLs.

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The relationship between RAPD markers and quantitative traits of caraway (Carum carvi L.)

Cited by 21 publications

References 16 publications

Assessment of Genetic Relationships in Breeding Lines and Cultivars of Brassica napus and Their Implications for Breeding Winter Oilseed Rape

Assessment of Genetic Relationships in Breeding Lines and Cultivars of Brassica napus and Their Implications for Breeding Winter Oilseed Rape

Identification of ISSR markers associated with valerenic acid content and antioxidant activity in Valeriana jatamansi Jones in the West Himalaya

Marker-trait association analysis for drought tolerance in smooth bromegrass

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