The relationship between plasma membrane lipid composition and physical-chemical properties. III. Detailed physical and biochemical analysis of fatty acid-substituted EL4 plasma membranes

Poon, Rebecca; Clark, William R.

doi:10.1016/0005-2736(82)90255-3

Cited by 15 publications

(2 citation statements)

References 30 publications

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“…The relationship between the fatty acid unsaturation and the fluidity of biological membranes is still a subject of controversy. In some studies the physical parameter under investigation remained unaffected by changes in the degree of unsaturation of fatty acids (Jahnig, 1979;Poon & Clark, 1982), and in others (York et al, 1982;Castuma & Brenner, 1983) variations in the physical properties that derived solely from alterations in the hydrocarbon chains have been reported.…”

Section: Relationship Between Lipid Composition and Physical Propertiesmentioning

confidence: 98%

“…Recent reviews concerning the influence of double bonds on membrane physical properties and function (Brenner, 1984;Stubbs & Smith, 1984) have emphasized the lack of a simple correlation between fatty acid unsaturation and fluidity of biomembranes. In some cases, changes in the fatty acid composition failed to reveal significant differences in the electron-spin-resonance order parameter (Poon & Clark, 1982) or DPH fluorescence anisotropy (Jahnig, 1979), whereas in others, the fluidity variations could be artefacts (Stubbs et al, 1980;Owen et al, 1982). Nevertheless, our study (Castuma & Brenner, 1983) has been cited (Stubbs & Smith, 1984) as one of the few in which the increase in the fluorescence anisotropy derived solely from alterations in the fatty acyl group composition.…”

Section: Introductionmentioning

confidence: 99%

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The influence of fatty acid unsaturation and physical properties of microsomal membrane phospholipids on UDP-glucuronyltransferase activity

Castuma

Brenner

1989

Biochemical Journal

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The relationship between lipid composition, the physical properties of microsomal phospholipids and the kinetics of liver UDP-glucuronyltransferase was studied in microsomes from guinea pigs supplied with a normal or a fat-free diet for 28 days. Fatty acid deficiency did not modify either the cholesterol/phospholipid molar ratio or the polar head group composition, but exclusively redistributed the unsaturated fatty acid pattern, by partially exchanging oleic for linoleic acid. This phenomenon accounts for the decrease of both rotational and translational mobilities of the fluorescent probes 1,6-diphenyl-1,3,5-hexatriene (DPH) and pyrene respectively. When the thermotropic behaviour of the different systems was assessed, no transition temperature (gel-liquid-crystalline) between 10 and 40 degrees C was seen as a consequence of the lower degree of unsaturation, either in the microsomal membranes or in the total lipid or total phospholipid extracts from the treated animals. In spite of this, the polarization ratio of trans-parinaric acid and the fluorescence intensity of merocyanine 540 revealed that a significant lateral phase separation occurred at 20-22 degrees C in the extracted phospholipids, which was smoother in the total lipid fractions and in the native microsomal membranes. Fatty acid deficiency caused an upward shift of the midpoint temperature of the lateral phase separation. Furthermore, the phosphatidylcholine extracted from the 'normal' microsomes showed a lateral phase separation centred at a lower temperature than that extracted from 'fat-deficient' microsomes. In contrast, the Arrhenius plot of UDP-glucuronyltransferase from 'normal' microsomes exhibited a change in slope at a higher temperature than that from treated microsomes. These results would suggest that fatty acid deficiency in guinea-pig liver microsomes, while rigidizing the bulk lipids, would segregate the most unsaturated phosphatidylcholine molecules towards the UDP-glucuronyltransferase microenvironment, in accordance with our previous results with cholesterol incorporation [Castuma & Brenner (1986) Biochemistry 25, 4733-4738].

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Section: Relationship Between Lipid Composition and Physical Propertiesmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

The influence of fatty acid unsaturation and physical properties of microsomal membrane phospholipids on UDP-glucuronyltransferase activity

Castuma

Brenner

1989

Biochemical Journal

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Effects of fatty acid deficiency on the lipid composition and physical properties of guinea pig rough endoplasmic reticulum

Soulages

Brenner

1987

Mol Cell Biochem

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Twenty-six days of fat deficiency brought about a decrease of linoleic and an increase of oleic acid in rough endoplasmic reticulum (RER) of guinea pig liver. Arachidonic acid was only slightly decreased in some phospholipids whereas eicose-5,8,11-trienoic acid was not enhanced except in phosphatidyl-inositol. All these changes were relevant specifically in phosphatidylinositol molecules and less important in phosphatidylcholine and phosphatidylethanolamine. Fat deficiency did not modify the relative proportion of phospholipids and cholesterol. Therefore, fat deficient guinea pig microsomes are a good model to study the effect of unsaturated fatty acids on membrane properties. Fluorescent anisotropy of RER membranes, lipids and phospholipids labeled with diphenylhexatriene, was increased by the fat deficiency. The most important increase was observed in liposomes of a mixture of RER phosphatidylinositol, phosphatidylserine and sphingomyelin. A small change was found in phosphatidylcholine and phosphatidylethanolamine dispersions at 37 degrees C. The modification of the lipid unsaturation evoked fluorescent anisotropy changes. Temperature-dependent fluorescent polarization curves of RER membranes labeled with trans-parinaric acid did not show inflections in the temperature range from 5 to 45 degrees C but, RER lipids and phospholipids presented a phase separation at about 20 degrees C. This inflection point was not modified by the fat deficient diet. In those liposomes prepared with a mixture of RER phosphatidylinositol, phosphatidylserine and sphingomyelin, the inflection point was produced at about 37 degrees C.

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Interface between membrane biology and clinical medicine

Zakim

1986

The American Journal of Medicine

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The relationship between plasma membrane lipid composition and physical-chemical properties. III. Detailed physical and biochemical analysis of fatty acid-substituted EL4 plasma membranes

Cited by 15 publications

References 30 publications

The influence of fatty acid unsaturation and physical properties of microsomal membrane phospholipids on UDP-glucuronyltransferase activity

The influence of fatty acid unsaturation and physical properties of microsomal membrane phospholipids on UDP-glucuronyltransferase activity

Effects of fatty acid deficiency on the lipid composition and physical properties of guinea pig rough endoplasmic reticulum

Interface between membrane biology and clinical medicine

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