Interface between membrane biology and clinical medicine

Zakim, David

doi:10.1016/0002-9343(86)90820-x

Cited by 20 publications

(3 citation statements)

References 54 publications

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“…Na /K ATPase is a marker of membrane function as it is an integral membrane protein, depending on the chemical-physical properties of the microenvironment for its activity 18) . Moreover, in association with membrane fluidity, it represents an index of membrane functionality, being involved in the modulation of phospholipid and protein interactions 40) . This study showed a decrease in platelet Na /K ATPase activity in cigarette smokers compared to controls.…”

Section: Discussionmentioning

confidence: 99%

Smoking-Induced Alterations in Platelet Membrane Fluidity and Na+/K+-ATPase Activity in Chronic Cigarette Smokers

Padmavathi

Reddy

Maturu³

et al. 2010

JAT

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Aim: Cigarette smoking is a recognized risk factor for cardiovascular diseases and has been implicated in the pathogenesis of atherosclerosis. Platelet adhesiveness and aggregation increases as a result of smoking. Cigarette smoking modifies haemostatic parameters via thrombosis with a consequently higher rate of cardiovascular events, but smoking-induced alterations of platelet membrane fluidity and other changes have not been studied. Methods: Thirty experimental and control subjects (mean age 35 8) were selected for the study. Experimental subjects had smoked 10 2 cigarettes per day for 7 − 10 years. The plasma lipid profile, platelet carbonyls, sulfhydryl groups, Na /K -ATPase activity, fluidity using a fluorescent probe 1,6-diphenyl-1,3,5-hexatriene (DPH), total cholesterol and phospholipids as well individual phospholipids were determined. Results: Increases in the platelet membrane cholesterol phospholipid (C/P) ratio, phosphotidylethanolamine, phosphotidylserine with decreased phosphotidylcholine, Na /K -ATPase activity, fluidity and no significant change in phosphotidylinositol and sphingomylein, as well as increases in plasma total cholesterol, LDL-cholesterol, protein carbonyls with decreased HDL-cholesterol and sulfhydryl groups were observed in cigarette smokers. Platelet membrane total phospholipids were positively correlated with plasma LDL-cholesterol (r 0.568) and VLDL-cholesterol (r 0.614) in cigarette smokers. Conclusions: Increased plasma LDL-cholesterol, VLDL-cholesterol and total cholesterol might have resulted in the increased C/P ratio and decreased platelet membrane fluidity of cigarette smokers. J Atheroscler Thromb, 2010; 17:619-627.

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Section: Discussionmentioning

confidence: 99%

Smoking-Induced Alterations in Platelet Membrane Fluidity and Na+/K+-ATPase Activity in Chronic Cigarette Smokers

Padmavathi

Reddy

Maturu³

et al. 2010

JAT

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“…Membrane fluidity, a submacroscopic property that is closely related to membrane molecular organization, is affected by various compositional factors such as the content in proteins, cholesterol and phospholipids, and their fatty acid composition (35). Thus, in association with membrane fluidity, Na + /K + ATPase represents an index of membrane functionality being involved in the modulation of phospholipid and protein interactions (36). In addition, data regarding TMA‐DPH anisotropy suggest that platelet membrane fluidity in obese patients was significantly lower than that in control subjects.…”

Section: Discussionmentioning

confidence: 99%

Interactions Between Lipoproteins and Platelet Membranes in Obesity

Raffaelli

Nanetti

D'Angelo

et al. 2009

Obesity

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The aim was to investigate low‐density lipoprotein (LDL) composition and Na+/K+ adenosine triphosphatase (ATPase) and Ca2+ ATPase activities and membrane fluidity measured by 1‐(4‐trimethylaminophenyl)‐6‐phenyl‐1,3,5‐hexatriene (TMA‐DPH) in platelets from obese patients and controls in order to identify, if any, platelet membrane's chemical–physical and/or functional modifications associated with compositional modification of circulating lipoproteins. Moreover, we studied the in vitro effect on both platelet transmembrane cationic transport and fluidity, by incubating LDL from 30 obese subjects with platelets from 30 control subjects. The analysis of the chemical composition of LDL from obese patients showed a significant increase in the percent content of total cholesterol (TC) and triglycerides (TGs) and in the mean levels of lipid hydroperoxides compared to controls' LDL. Platelet Na+/K+ ATPase and Ca2+ ATPase activities showed, respectively, a significant decrease and increase in patients compared to controls; minor significant, respectively, decreases and increases are shown also in control platelets incubated with LDL from obese patients. Anisotropy tested with TMA‐DPH probe was significantly increased both in platelets from obese patients and in control platelets incubated with LDL from obese patients compared to control platelets. This study highlights that obesity induces remarkable modifications both in lipoproteins and platelets. Both platelet hyperfunction and quantitative/qualitative alterations in plasma lipoproteins, as well as an altered interaction between circulating lipoproteins and platelets, might play a relevant role in the increased prevalence of the early atherosclerotic lesions development in obese subjects. The present data point out that obesity might represent a major potentially modifiable risk factor for the onset of numerous complications, in particular cardiovascular ones.

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“…For this purpose, we selected Na 1 / K 1 -ATPase activity and membrane fluidity as potential markers of cytotoxicity. Na 1 /K 1 -ATPase activity was selected because it plays a major role in the cellular osmotic regulation (Mazzanti et al, 1997), while membrane fluidity was selected because it is a key determinant of membrane function by modulating phospholipid-protein interactions (Zakim, 1986).…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic effects induced by hexachlorobenzene in Squilla mantis (L.) (Crustacea, Stomatopoda)

Dell’Anno

Raffaelli

Danovaro

et al. 2008

Environmental Toxicology

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Contamination of marine environments by hexachlorobenzene (HCB) represents a serious concern for potential consequences on ecosystem and human health. Despite this, information on cytotoxic effects on marine organisms is still largely lacking. In this study, we investigated cytotoxic effects induced by HCB on gonads and muscular tissue of Squilla mantis by analysing Na 1 /K 1 -ATPase activity and plasma membrane fluidity. This crustacean species was selected as a model for its habitat, trophic level, feeding behavior, and commercial exploitation for human consumption. Time course experiments revealed that low concentrations of HCB (i.e. 50 nM) determine an exponentially decrease of Na 1 /K 1 -ATPase activity and a significant modification of cellular membrane fluidity. Significant negative relationships between Na 1 /K 1 -ATPase activity and membrane fluidity were observed, suggesting that changes in the structure and packing of cellular membranes induced by HCB may be the primary factor affecting the activity of essential bilayer-associated enzymes. Overall these findings suggest that even small concentrations of HCB may determine important changes on cell metabolism with potential cascade effects on recruitment of this commercial species.

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Interface between membrane biology and clinical medicine

Cited by 20 publications

References 54 publications

Smoking-Induced Alterations in Platelet Membrane Fluidity and Na+/K+-ATPase Activity in Chronic Cigarette Smokers

Smoking-Induced Alterations in Platelet Membrane Fluidity and Na+/K+-ATPase Activity in Chronic Cigarette Smokers

Interactions Between Lipoproteins and Platelet Membranes in Obesity

Cytotoxic effects induced by hexachlorobenzene in Squilla mantis (L.) (Crustacea, Stomatopoda)

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