The relationship between phagocytosis and intracellular killing of Staphylococcus aureus by bovine neutrophils

Williams, M.R.; Craven, N.; Field, Terence R.; Bunch, Kathryn

doi:10.1016/0007-1935(85)90085-5

Cited by 26 publications

(20 citation statements)

References 12 publications

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“…This is in contrast with other studies [25], where PMN, with a high rate of phagocytosis, more efficiently killed the internalised S. aureus.…”

Section: % Overall Killing % Overall Killingcontrasting

confidence: 99%

“…Moreover, a significant positive correlation (r = 0.50; P < 0.001) was found between overall killing and PMA-stimulated CL [13]. This is in agreement with the results obtained by Williams et al [25], who demonstrated that intracellular survival but not phagocytosis of S. aureus was correlated with reduced CL by bovine PMN. It was demonstrated by Vandeputte-Van Messom et al [24], that blood PMN from cows with a low reactive oxygen species generating capacity has low circulating glucose levels.…”

Section: % Overall Killing % Overall Killingsupporting

confidence: 92%

“…Williams et al [25] found that with an increasing bacteria:neutrophil ratio, the number of phagocytosed bacteria increased proportionately but the intracellular killing was impaired. The observed variation in the ratio (between 4:1 and 5:1) used in this study was not large enough to explain the observed impairment of overall killing during early lactation.…”

Section: % Overall Killing % Overall Killingmentioning

confidence: 99%

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Effect of milk sampling techniques on milk composition, bacterial contamination, viability and functions of resident cells in milk

et al. 2001

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-Three different milk sampling techniques were evaluated during milk sampling: a direct aseptic collection from the udder through a sterile cannula was used as the reference technique, compared with either a manual or a mechanical sampling method. In this study 30 high-yielding HolsteinFriesian dairy cows at different stages of lactation and free of udder infection were used. For each milk sample, the influence of milk sampling techniques was determined for the following parameters: somatic cell count, milk composition, bacterial contamination, viability, in vitro phagocytosis and overall killing of Staphylococcus aureus Newbould 305, and cellular chemiluminescence. Because milk sampling occurred throughout lactation, the differences between early, mid-and late lactation were estimated. It was concluded that bacterial contamination was not significantly different in manual milking samples and the reference technique; bacterial contamination was, however, significantly (P < 0.001) higher in machine milking samples than in the reference technique. Among the different sampling techniques, no significant effects on SCC, milk composition, viability and functions of the cells isolated from milk were observed. It was found that viability, intracellular killing and cellular chemiluminescence of milk PMN were significantly lower (P < 0.05) in early lactation compared to mid-lactation. Phagocytosis was significantly (P < 0.05) higher in early lactation compared to mid-and late lactation, and no significant differences were observed between mid-and late lactation. From this study, it can be concluded that despite a higher bacterial contamination obtained with the mechanical sampling method, the 3 milk sampling techniques described in this study can be used for the evaluation of milk cell functions. dairy cow / milk sampling technique / stage of lactation / milk composition / milk neutrophil functions Vet. Res. 32 (2001) 565-579 565

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“…This is in contrast with other studies [25], where PMN, with a high rate of phagocytosis, more efficiently killed the internalised S. aureus.…”

Section: % Overall Killing % Overall Killingcontrasting

confidence: 99%

Section: % Overall Killing % Overall Killingsupporting

confidence: 92%

Section: % Overall Killing % Overall Killingmentioning

confidence: 99%

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Effect of milk sampling techniques on milk composition, bacterial contamination, viability and functions of resident cells in milk

et al. 2001

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“…6571) was used in bactericidal assays. Bacteria were prepared and bactericidal assays performed as described by Williams et al (1985), with human in place of bovine neutrophils. Lysostaphin was used to remove extracellular staphylococci in the determination of intracellular survival (Tan et al, 1971).…”

Section: Bactericidal Assaysmentioning

confidence: 99%

“…Total and intracellular survival and phagocytosis were calculated as described by Williams et al (1985). Total survival is expressed as the percentage of viable bacteria present at the end of the incubation, phagocytosis as the percentage of bacteria ingested by neutrophils (allowing for those adherent to neutrophils but not internalized), and intracellular survival as the percentage of those bacteria actually phagocytosed which survive intracellularly.…”

Section: Bacterial Countingmentioning

confidence: 99%

The effect of the NADPH oxidase inhibitor diphenyleneiodonium on aerobic and anaerobic microbicidal activities of human neutrophils

Ellis

Mayer

Jones

1988

Biochemical Journal

158

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NADPH-dependent superoxide production by intact human neutrophils is inhibited by DPI (diphenyleneiodonium), when stimulated by either FMLP (N-formylmethionyl-leucyl-phenylalanine) or PMA (phorbol 12-myristate 13-acetate). Addition of 10 microM-DPI abolished the reduction of both the FAD and the cytochrome b components of the NADPH oxidase. DPI inhibition of the oxidase was associated with defective aerobic killing of staphylococci by human neutrophils. Anaerobic killing, phagocytosis, chemotaxis and motility were relatively unaffected by 10 microM-DPI. Degranulation of the azurophil and specific granules, induced by the soluble stimuli FMLP or PMA, and by particulate stimuli was decreased by the presence of DPI. The above effects of DPI on human neutrophils are similar to those found in chronic granulomatous disease.

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A comparative study of bovine blood and milk neutrophil functions with luminol‐dependent chemiluminescence

et al. 2001

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In this study, a technique was developed for the chemiluminescence (CL) measurement of bovine milk polymorphonuclear leukocytes (PMN). In the first study, the effects of cell number and the concentration of phorbol-12-myristate-13-acetate (PMA), luminol, latex bead particles, dimethyl sulphoxide (DMSO) and gelatin on the luminol-dependent cellular CL (LDCL) response were assessed with healthy cows in different stages of lactation. In the second study, the LDCL and in vitro bactericidal activity of blood and milk PMN towards Staphylococcus aureus was investigated. In general, the CL activity of blood PMN was consistently higher than that of milk PMN. We found that (a) the optimal cell density in blood and milk cells for maximal LDCL response ranged from 1.5 x 10(6) to 5 x 10(6) cells/mL; (b) the optimal concentrations of PMA, latex beads and luminol for maximal LDCL response were 100-200 ng/ml, 500 particles/PMN and 0.1 mmol/L, respectively. Concentrations of DMSO of 0.5-1% (v/v) did not significantly affect the maximal CL response of PMN. Gelatin concentrations of 0.1 -0.5 mg/ml had no effect on the LDCL of PMN. In addition, the LDCL of PMN was significantly correlated with bactericidal activity towards S. aureus (r = 0.78, p < 0.001 for blood PMN and r = 0.66, p < 0.01 for milk PMN). Under the optimal experimental conditions for measurement of CL produced by bovine blood and milk PMN defined in this study, LDCL assay is an accurate and reproducible technique for the rapid quantification of PMN bactericidal activity in physiological and pathological conditions of high-yielding dairy cows.

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The relationship between phagocytosis and intracellular killing of Staphylococcus aureus by bovine neutrophils

Cited by 26 publications

References 12 publications

Effect of milk sampling techniques on milk composition, bacterial contamination, viability and functions of resident cells in milk

Effect of milk sampling techniques on milk composition, bacterial contamination, viability and functions of resident cells in milk

The effect of the NADPH oxidase inhibitor diphenyleneiodonium on aerobic and anaerobic microbicidal activities of human neutrophils

A comparative study of bovine blood and milk neutrophil functions with luminol‐dependent chemiluminescence

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