The Relationship between Lifespan of Marine Bivalves and Their Fatty Acids of Mitochondria Lipids

Abstract

Marine bivalves belonging to the Mytilidae and Pectinidae Families were used in this research. The specific objectives of this study were: to determine the Fatty Acids (FAs) of mitochondrial gill membranes in bivalves with different lifespans, belonging to the same family, and to calculate their peroxidation index; to compare the levels of ROS generation, malondialdehyde (MDA), and protein carbonyls in the mitochondria of gills, in vitro, during the initiation of free-radical oxation; to investigate whether the FAs of mitochondria gill membranes affect the degree of their oxidative damage and the maximum lifespan of species (MLS). The qualitative membrane lipid composition was uniform in the studied marine bivalves, regardless of their MLS. In terms of the quantitative content of individual FAs, the mitochondrial lipids differed significantly. It is shown that lipid matrix membranes of the mitochondria of long-lived species are less sensitive to in vitro-initiated peroxidation compared with the medium and short-lived species. The differences in MLS are related to the peculiarities of FAs of mitochondrial membrane lipids.

Effect of Polystyrene Microplastics on the Antioxidant System and Immune Response in GIFT (Oreochromis niloticus)

“…The WST-1 method was used to measure SOD concentrations through nitro blue tetrazolium inhibition at 450 nm. ROS levels were determined by the oxidation of DHR 123 (dihydrorhodamine 123) to fluorescent rhodamine 123 [23], and without MDA determination according to previous studies [24]. The enzyme activities of IL-1β and TNF-α were determined by diagnostic reagent kits (Comin Biotechnology Co., Ltd., Suzhou, China), and the determination and calculation methods were completed in accordance with previous studies [25,26].…”