The Relationship between Imbibitional Damage and Initial Water Content of Soybeans

Ishida, Noriko; Kano, Hiromi; Kobayashi, Toshio; Hamaguchi, Hideo; Yoshida, Takashi

doi:10.1080/00021369.1988.10869127

Cited by 4 publications

(6 citation statements)

References 2 publications

(2 reference statements)

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“…Quando a semente ou eixo embrionário é colocado em água pura a embebição processa-se muito rapidamente, podendo ocorrer danos ou injúrias às sementes (Powell & Matthews, 1979). Os efeitos prejudiciais da rápida embebição podem ser decorrentes de fatores como: redução na integridade das membranas celulares, aumento na atividade de microrganismos, pelo vazamento de solutos, ou, ainda, baixa disponibilidade de oxigênio, levando ao processo de respiração anaeróbica (Ishida et al, 1988;Armstrong & McDonald, 1992).…”

Section: Resultsunclassified

Avaliação da qualidade fisiológica de sementes de soja, após o processo de hidratação-desidratação e envelhecimento acelerado

Braccini

Reis

Sediyama

et al. 1999

Pesq. agropec. bras.

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e MARIA DO CARMO LANA BRACCINI 4 RESUMO -Foi conduzido um experimento com o objetivo de avaliar a qualidade fisiológica de sementes de soja (Glycine max (L.) Merrill) , envolvendo as variedades UFV-10, IAC-8 e Doko RC, submetidas ao processo de hidratação-desidratação, seguido por uma condição de estresse induzida pelo teste de envelhecimento acelerado. As sementes foram pré-condicionadas em PEG 6000, com potencial osmótico de -0,8 MPa, a uma temperatura de 20 o C, por quatro dias. Em seguida, as sementes foram novamente desidratadas, até atingir o conteúdo de umidade inicial, sendo então submetidas ao teste de envelhecimento acelerado pelos seguintes períodos: 0, 24, 48 e 72 horas. As sementes pré-condicionadas foram comparadas com duas testemunhas: sementes não-tratadas e sementes embebidas em água. Após cada período de envelhecimento, as sementes foram avaliadas pelo teste padrão de germinação, comprimento de plântula e biomassa seca das plântulas. Em casa de vegetação, determinaram-se o índice de velocidade de emergência e a emergência final em substrato de areia. O tratamento de hidratação-desidratação em solução de PEG 6000 melhorou o desempenho das sementes em termos de germinação e vigor. O aumento nos períodos de envelhecimento acelerado influenciou negativamente o desempenho das sementes, em todos os tratamentos avaliados. Contudo, o tratamento com PEG 6000 foi satisfatório em manter a qualidade das sementes. A embebição das sementes de soja em água desmineralizada prejudicou a qualidade fisiológica. As variedades de soja mostraram resposta diferenciada aos tratamentos de hidratação-desidratação e aos períodos de envelhecimento acelerado.Termos para indexação: Glycine max, germinação, vigor, estresse hídrico, condicionamento osmótico. SOYBEAN PHYSIOLOGICAL SEED QUALITY EVALUATION AFTER HYDRATION-DEHYDRATION PROCESS AND ACCELERATED AGINGABSTRACT -A work was carried out with the purpose of evaluating the physiological quality of osmoconditioned soybean (Glycine max (L.) Merrill) seeds of UFV-10, IAC-8 and Doko RC varieties, in terms of response to stress induced by the accelerated aging test. Seeds were osmoconditioned in PEG 6000, with an osmotic potential of -0.8 MPa and 20 o C of temperature, for four days. After that, seeds were dried back to the initial moisture content and submitted to the accelerated aging test for the following periods: 0, 24, 48 and 72 hours. Two controls were used: untreated seeds and seeds soaked in water. After several determined accelerated aging periods, seeds were evaluated by the standard germination test, seedling length and dry biomass of seedlings. In greenhouse the speed of emergence-index and the final emergence in sand seedbed were determined. Soybean seeds hydration-dehydration treatment in PEG 6000 solution improved seed performance in relation to germination and vigor. The extent of the accelerated aging period affected seed performance in all parameters evaluated. However, PEG 6000 treatment was satisfactory in maintaining the seed quality during the accelerated...

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Section: Resultsunclassified

Avaliação da qualidade fisiológica de sementes de soja, após o processo de hidratação-desidratação e envelhecimento acelerado

Braccini

Reis

Sediyama

et al. 1999

Pesq. agropec. bras.

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“…The damage to tissues is severe in seeds with initial water concentrations under 10 %. However, when the seed moisture content before imbibition is 12–17 % there is an alleviation of damage, associated with a decline in the leakage of solutes, the appearance of respiratory activity, and clear phosphate signals in 31 P-NMR spectra (Pollock, 1969; Pollock et al , 1969; Hobbs and Obendorf, 1972; Ashworth and Obendorf, 1980; Vertucci and Leopold, 1984; Ishida et al , 1988 a , b ). This indicates that membrane functions are restored, even though the activities of respiration and metabolism are restricted.…”

Section: Discussionmentioning

confidence: 99%

“…This damage takes place in the early stages of imbibition (Parrish and Leopold, 1977) not only on the cotyledons but also on the embryonic axis (Ashworth and Obendorf, 1980; McDonald et al , 1988 b ). Seeds do not, however, suffer from soaking effects when the water concentration in seeds is 12–17 % (Pollock et al , 1969; Hobbs and Obendorf, 1972; Ashworth and Obendorf, 1980; Ishida et al , 1988 a ). Alleviation of the effects of soaking injury as a result of the increase in the moisture content of seeds before imbibition is related to the reduced binding energy of water molecules and the appearance of respiratory activity (Vertucci and Leopold, 1984; Ishida et al , 1988 b ).…”

Section: Introductionmentioning

confidence: 99%

Role of Seed Coat in Imbibing Soybean Seeds Observed by Micro-magnetic Resonance Imaging

et al. 2008

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Background and AimsImbibition of Japanese soybean (Glycine max) cultivars was studied using micro-magnetic resonance imaging (MRI) in order to elucidate the mechanism of soaking injury and the protective role of the seed coat.MethodsTime-lapse images during water uptake were acquired by the single-point imaging (SPI) method at 15-min intervals, for 20 h in the dry seed with seed coat, and for 2 h in seeds with the seed coat removed. The technique visualized water migration within the testa and demonstrated the distortion associated with cotyledon swelling during the very early stages of water uptake.Key ResultsWater soon appeared in the testa and went around the dorsal surface of the seed from near the raphe, then migrated to the hilum region. An obvious protrusion was noted when water reached the hypocotyl and the radicle, followed by swelling of the cotyledons. A convex area was observed around the raphe with the enlargement of the seed. Water was always incorporated into the cotyledons from the abaxial surfaces, leading to swelling and generating a large air space between the adaxial surfaces. Water uptake greatly slowed, and the internal structures, veins and oil-accumulating tissues in the cotyledons developed after the seed stopped expanding. When the testa was removed from the dry seeds before imbibition, the cotyledons were severely damaged within 1·5 h of water uptake.ConclusionsThe activation of the water channel seemed unnecessary for water entry into soybean seeds, and the testa rapidly swelled with steeping in water. However, the testa did not regulate the water incorporation in itself, but rather the rate at which water encountered the hypocotyl, the radicle, and the cotyledons through the inner layer of the seed coat, and thus prevented the destruction of the seed tissues at the beginning of imbibition.

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“…10 and 20%, the amount of leachate from seeds by soaking in water decreased with increasing initial water contents and became almost negligible above 20%. 1) These facts mean that the physical states of water in seeds at the initiation of imbibition in the stage of germination have a close relationship to the imbibitional effect and the release of stored materials. In this paper we have investigated the characteristics of water in soybean seeds in relation to the mechanism ofimbibitional damage, which was deduced by another experiment concerning mechanical destruction of seed structure by rapid water absorption.…”

mentioning

confidence: 99%

“…In this paper we have investigated the characteristics of water in soybean seeds in relation to the mechanism ofimbibitional damage, which was deduced by another experiment concerning mechanical destruction of seed structure by rapid water absorption. 1 ) …”

mentioning

confidence: 99%

Analysis of Physical States of Water in Soybean Seeds by NMR

Ishida

Kano²,

Kobayashi

et al. 1988

Agricultural and Biological Chemistry

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The Relationship between Imbibitional Damage and Initial Water Content of Soybeans

Cited by 4 publications

References 2 publications

Avaliação da qualidade fisiológica de sementes de soja, após o processo de hidratação-desidratação e envelhecimento acelerado

Avaliação da qualidade fisiológica de sementes de soja, após o processo de hidratação-desidratação e envelhecimento acelerado

Role of Seed Coat in Imbibing Soybean Seeds Observed by Micro-magnetic Resonance Imaging

Analysis of Physical States of Water in Soybean Seeds by NMR

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