The Relationship Between Human Semen Characteristics and Sperm Apoptosis: A Pilot Study

Chen, Z.

doi:10.2164/jandrol.05073

Cited by 53 publications

(48 citation statements)

References 37 publications

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“…Zini et al [39] demonstrated negative correlations between sperm DNA integrity and sperm quality in partners of couples attending IVF clinics. Additionally, in a small cohort of patients consulting for infertility investigation, prospective analyzes showed that both sperm motility and sperm morphology were inversely correlated to the rate of nuclear DNA fragmentation [10][11][12]36]. These results corroborated with our findings and suggested that sperm motility and morphology defects may be in part due to a reduction of sperm DNA integrity which can be promutagenic and can adversely affect fetal outcome after IVF and has lifelong implications on health of child [34].…”

Section: Discussionsupporting

confidence: 88%

Effects of reduced seminal enzymatic antioxidants on sperm DNA fragmentation and semen quality of Tunisian infertile men

Atig

Kerkeni

Saâd

et al. 2013

J Assist Reprod Genet

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Purpose To evaluate levels of sperm DNA fragmentation and enzymatic antioxidant status in seminal plasma of Tunisian fertile and infertile men in order to assess the effects of seminal oxidative stress on sperm DNA integrity and semen quality. Methods Semen samples from 100 infertile patients (40 oligoasthenoteratozoospermics, 31 teratozoospermics and 29 asthenozoospermics) and 50 fertile men (controls) were analyzed for DNA fragmentation by TUNEL assay and biochemical parameters. Seminal antioxidant activities (Superoxide dismutase, Glutathione peroxidase and Catalase) and malondialdehyde concentrations were measured spectrophotometrically.Results Sperm DNA fragmentation and malondialdehyde levels in infertile groups were more elevated than controls. Nevertheless, the activities of the antioxidant enzymes were significantly lower in abnormal groups compared to normozoospermics. Sperm DNA fragmentation was closely and positively correlated to malondialdehyde levels (r=0.37, P=0.008); meanwhile, reduced seminal antioxidant profile was negatively associated to sperm DNA fragmentation. Interestingly, we noted also that sperm DNA fragmentation was negatively correlated to sperm motility (r=−0.54, P<0.001) and positively associated to the abnormal sperm morphology (r=0.57, P=0.002). Conclusions This report revealed that increased sperm DNA fragmentation can be due to the impaired seminal enzymatic antioxidant profile and increased Lipid peroxidation. Our results sustain that the evaluation of sperm DNA fragmentation and seminal oxidative biomarkers in infertile men is recommended as a consistent prognostic tool for male infertility assessment.

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Section: Discussionsupporting

confidence: 88%

Effects of reduced seminal enzymatic antioxidants on sperm DNA fragmentation and semen quality of Tunisian infertile men

Atig

Kerkeni

Saâd

et al. 2013

J Assist Reprod Genet

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“…A significant negative relationship between the proportions of apoptotic sperm and the proportions of sperm with normal morphology in semen applying the Tygerberg's strict criteria (Kruger et al, 1988) has been reported (Chen et al, 2006). In another study normal sperm morphology assessed in the swim-up preparations correlated negatively with chromatin fragmentation only in teratozoospermic semen samples but not in normospermic ones as determined by the WHO 1992 criteria (Muratori et al, 2003).…”

Section: Function and Relevance Of Caspase Activation In Spermatozoa mentioning

confidence: 93%

“…Several studies investigated the relationship between sperm morphology and apoptosis (Aziz et al, 2007;Chen et al, 2006;Gandini et al, 2000;Ricci et al, 2002;Sakkas et al, 2002;Shen et al, 2002;Siddighi et al, 2004). The design of these studies might not have facilitated the accurate distinction between moribund or necrotic sperm and motile sperm expressing apoptotic markers.…”

Section: Function and Relevance Of Caspase Activation In Spermatozoa mentioning

confidence: 99%

“…Other studies correlated sperm morphology in neat semen with apoptosis in selected motile sperm subpopulation obtained after swim-up or double gradient centrifugation techniques (Almeida et al, 2005;Benchaib et al, 2003;Muratori et al, 2003;Weng et al, 2002). All these studies applied different criteria for the assessment of sperm morphology including WHO 1992 standards (Muratori et al, 2003), WHO 1999 standards (Almeida et al, 2005;Benchaib et al, 2003;Weng et al, 2002) and the Tygerberg's strict criteria (Chen et al, 2006;Sakkas et al, 2002;Siddighi et al, 2004;Weng et al, 2002).…”

Section: Function and Relevance Of Caspase Activation In Spermatozoa mentioning

confidence: 99%

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Impact of caspase activation in human spermatozoa

Grunewald

Sharma

Paasch

et al. 2009

Microscopy Res & Technique

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Caspases are central components in the apoptosis signaling cascade. The family of cysteine proteases transduces and enhances the apoptosis signal, and activation of effector caspases results in controlled cellular degradation. Although initially the presence of caspases in spermatozoa was controversially discussed in recent years, many studies demonstrated their activation in male germ cells. Activated apoptosis signaling results in decreased fertilizing capacity of the sperm. This review presents the current knowledge on the role of caspases in human sperm. Techniques of caspase monitoring are highlighted. With regard to the high impact of caspases on the sperm fertilizing potential, physiological and pathological settings of caspase activation and inactivation are discussed. Finally, the effects of depletion of caspase-positive sperm are shown with various standard and molecular sperm preparation methods. Microsc. Res. Tech. 00:000-000, 2009. V

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“…In infertile patients, an increased incidence of chromosome anomalies [Carrell 2008;Sarrate et al 2010], and parallel elevated percentages of apoptosis in spermatozoa have been described [Tesarik et al 1998;Carrell et al 2003;Chen et al 2006]. The chromosome anomalies are related to synapsis abnormalities, and recombination and segregation in germ cells [Topping et al 2006].…”

Section: Introductionmentioning

confidence: 99%

A sequential methodology that allows apoptotic cell sorting and FISH analysis in human testicular cells

Garcia-Quevedo¹,

Sarrate²,

Vidal³

et al. 2012

Systems Biology in Reproductive Medicine

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A sequential methodology that allows apoptotic cell sorting and FISH analysis in human testicular cells, Systems Biology in Reproductive Medicine, 58:6, 354-361, DOI: 10.3109/19396368.2012.717163 To link to this article: https://doi.org/10. 3109/19396368.2012.717163 The objective of this study was to develop a methodology that permits the detection and separation of apoptotic cells in human testicular tissue and their subsequent cytogenetic analysis by fluorescence in situ hybridization (FISH). The sequential methodology consisted of five steps: 1) enzymatic disaggregation of testicular tissue, 2) specific staining of apoptotic cells, 3) cell sorting by flow cytometry, 4) cell fixation, and 5) FISH. Enzymatic disaggregation yielded cell counts that ranged from 1.7x10 5 to 5x10 6 cells, and viability values greater than 72%. The apoptotic (mean ± SD: 22% ± 5.3%) and viable (45.5% ± 7.3%) populations were identified and selected by flow cytometry and demonstrated purity values ranging between 62% and 100%. The paraformaldehyde fixation of the selected fractions resulted in cell loss values of less than 10%. The application of three treatments before FISH (membrane permeabilization, elimination of cytoplasmic components, and re-fixation of the sample) resulted in hybridization frequencies of greater than 98%. In both selected fractions, cells of all spermatogenic stages and Sertoli cells were identified. The methodology developed has enabled the preparation of a cellular suspension with optimal viability and counting, the efficient selection of the apoptotic population, and its analysis by cytogenetic techniques. The application of this methodology in testicular cells should help establish whether there is a direct relationship between chromosome anomalies and apoptosis.

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The Relationship Between Human Semen Characteristics and Sperm Apoptosis: A Pilot Study

Cited by 53 publications

References 37 publications

Effects of reduced seminal enzymatic antioxidants on sperm DNA fragmentation and semen quality of Tunisian infertile men

Effects of reduced seminal enzymatic antioxidants on sperm DNA fragmentation and semen quality of Tunisian infertile men

Impact of caspase activation in human spermatozoa

A sequential methodology that allows apoptotic cell sorting and FISH analysis in human testicular cells

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