The Relationship between Circulating Ecdysteroids and Chela Allometry in Male Tanner Crabs: Evidence for a Terminal Molt in the Genus Chionoecetes

Tamone, Sherry L.; Taggart, S. James; Andrews, Alexander G.; Mondragon, Jennifer; Nielsen, Julie K.

doi:10.1651/s-2802.1

Cited by 23 publications

(15 citation statements)

References 27 publications

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“…YOs were harvested from intact (Day 0) animals and from ESA animals at various intervals post-ESA. Hemolymph samples (100 μl) were taken at the time of harvest and combined with 300 μl methanol for enzyme-linked immunosorbent assay (ELISA) analysis modified from Kingan (Kingan, 1989) and Tamone et al (Tamone et al, 2007) (see Appendix).…”

Section: Animals and Experimental Treatmentsmentioning

confidence: 99%

Molt regulation in green and red color morphs of the crab,Carcinus maenas: gene expression of molt-inhibiting hormone signaling components

Abuhagr

Blindert

Nimitkul

et al. 2013

Journal of Experimental Biology

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There was an error published in J. Exp. Biol. 217, In Fig. 3, the letters and numbers indicating which means were significantly different are missing from panel A. The correct figure is printed below.We apologise to the authors and readers for this omission. ) characteristic of late premolt animals and animals did not molt by 90 days post-ESA. There was no effect of ESA or MLA on the expression of Cm-elongation factor 2 (EF2), Cm-NOS, the beta subunit of GC-I (Cm-GC-Iβ), a membrane receptor GC (Cm-GC-II) and a soluble NO-insensitive GC (Cm-GC-III) in green morphs. Red morphs were affected by prolonged ESA and MLA treatments, as indicated by large decreases in Cm-EF2, Cm-GC-II and Cm-GC-III mRNA levels. ESA accelerated the transition of green morphs to the red phenotype in intermolt animals. ESA delayed molting in premolt green morphs, whereas intact and MLA animals molted by 30 days post treatment. There were significant effects on YO gene expression in intact animals: Cm-GC-Iβ mRNA increased during premolt and Cm-GC-III mRNA decreased during premolt and increased during postmolt. Cm-MIH transcripts were detected in eyestalk ganglia, the brain and the thoracic ganglion from green intermolt animals, suggesing that MIH in the brain and thoracic ganglion prevents molt induction in green ESA animals.

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Section: Animals and Experimental Treatmentsmentioning

confidence: 99%

Molt regulation in green and red color morphs of the crab,Carcinus maenas: gene expression of molt-inhibiting hormone signaling components

Abuhagr

Blindert

Nimitkul

et al. 2013

Journal of Experimental Biology

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“…Hemolymph Ecd concentrations being cyclic repeatedly rise and fall during the molt cycle of several crustacean species including the blue crab, C. sapidus [4], the American lobster, Homarus americanus [11], and the tanner crab, Chionoecetes bairdi [12]. Although the hemolymph Ecd concentrations differ by species, the described pattern is that animals at the IM stage have low levels of Ecd ranging from 0 ng/mL-100 ng/mL, while those at PM have much higher levels with 200 ng/mL to 2500 ng/mL [11].…”

Section: Introductionmentioning

confidence: 99%

Understanding molt control switches: Transcriptomic and expression analysis of the genes involved in ecdysteroidogenesis and cholesterol uptake pathways in the Y-organ of the blue crab, Callinectes sapidus

et al. 2021

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The crustacean molting process is regulated by an interplay of hormones produced by the eyestalk ganglia and Y-organs (YO). Molt-inhibiting hormone and crustacean hyperglycemic hormone released by the sinus gland of the eyestalk ganglia (EG) inhibit the synthesis and secretion of ecdysteroid by the YO, hence regulating hemolymph levels during the molt cycle. The purpose of this study is to investigate the ecdysteroidogenesis pathway, specifically genes linked to changes in ecdysteroid levels occurring at early premolt (ePM). To this end, a reference transcriptome based on YO, EG, and hepatopancreas was de novo assembled. Two genes (cholesterol 7-desaturase Neverland and cytochrome p450 307a1-like Spook) involved in ecdysteroidogenesis were identified from the YO transcriptome using sequence comparisons and transcript abundance. Two other candidates, Hormone receptor 4 and probable cytochrome p450 49a1 potentially involved in ecdysteroidogenesis were also identified. Since cholesterol is the ecdysteroid precursor, a putative cholesterol carrier (Apolipoprotein D-like) was also examined to understand if cholesterol uptake coincided with the increase in the ecdysteroid levels at the ePM stage. The expression level changes of the five candidate genes in the YO were compared between intermolt (IM) and induced ePM (iePM) stages using transcriptomic analysis. Expression analysis using qPCR were carried out at IM, iePM, and normal ePM. The increase in Spook and Neverland expression in the YO at the ePM was accompanied by a concomitant rise in ecdysteroid levels. The data obtained from iePM stage were congruent with those obtained from the normal ePM stage of intact control animals. The present findings support the role of Halloween genes in the ecdysteroidogenesis and molt cycle in the blue crab, Callinectes sapidus.

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“…Tanner crabs molt about 6 times in their first year with molting rates decreasing with crab size [37]. Both male and female Tanner crabs undergo terminal molts [38], [39] which for males may take place after18 molts and 12 years [35]. The maximum age of Tanner crabs is unknown but estimates range from 14–20 y [40].…”

Section: Introductionmentioning

confidence: 99%

Effects of Ocean Acidification on Juvenile Red King Crab (Paralithodes camtschaticus) and Tanner Crab (Chionoecetes bairdi) Growth, Condition, Calcification, and Survival

et al. 2013

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Ocean acidification, a decrease in the pH in marine waters associated with rising atmospheric CO2 levels, is a serious threat to marine ecosystems. In this paper, we determine the effects of long-term exposure to near-future levels of ocean acidification on the growth, condition, calcification, and survival of juvenile red king crabs, Paralithodes camtschaticus, and Tanner crabs, Chionoecetes bairdi. Juveniles were reared in individual containers for nearly 200 days in flowing control (pH 8.0), pH 7.8, and pH 7.5 seawater at ambient temperatures (range 4.4–11.9 °C). In both species, survival decreased with pH, with 100% mortality of red king crabs occurring after 95 days in pH 7.5 water. Though the morphology of neither species was affected by acidification, both species grew slower in acidified water. At the end of the experiment, calcium concentration was measured in each crab and the dry mass and condition index of each crab were determined. Ocean acidification did not affect the calcium content of red king crab but did decrease the condition index, while it had the opposite effect on Tanner crabs, decreasing calcium content but leaving the condition index unchanged. This suggests that red king crab may be able to maintain calcification rates, but at a high energetic cost. The decrease in survival and growth of each species is likely to have a serious negative effect on their populations in the absence of evolutionary adaptation or acclimatization over the coming decades.

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The Relationship between Circulating Ecdysteroids and Chela Allometry in Male Tanner Crabs: Evidence for a Terminal Molt in the Genus Chionoecetes

Cited by 23 publications

References 27 publications

Molt regulation in green and red color morphs of the crab,Carcinus maenas: gene expression of molt-inhibiting hormone signaling components

Molt regulation in green and red color morphs of the crab,Carcinus maenas: gene expression of molt-inhibiting hormone signaling components

Understanding molt control switches: Transcriptomic and expression analysis of the genes involved in ecdysteroidogenesis and cholesterol uptake pathways in the Y-organ of the blue crab, Callinectes sapidus

Effects of Ocean Acidification on Juvenile Red King Crab (Paralithodes camtschaticus) and Tanner Crab (Chionoecetes bairdi) Growth, Condition, Calcification, and Survival

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