Molt regulation in green and red color morphs of the crab,Carcinus maenas: gene expression of molt-inhibiting hormone signaling components

Abstract

There was an error published in J. Exp. Biol. 217, In Fig. 3, the letters and numbers indicating which means were significantly different are missing from panel A. The correct figure is printed below.We apologise to the authors and readers for this omission. ) characteristic of late premolt animals and animals did not molt by 90 days post-ESA. There was no effect of ESA or MLA on the expression of Cm-elongation factor 2 (EF2), Cm-NOS, the beta subunit of GC-I (Cm-GC-Iβ), a membrane receptor GC (Cm-GC-II) and a soluble NO-insensitive GC (Cm-GC-III) in green morphs. Red morphs were affected by prolonged ESA and MLA treatments, as indicated by large decreases in Cm-EF2, Cm-GC-II and Cm-GC-III mRNA levels. ESA accelerated the transition of green morphs to the red phenotype in intermolt animals. ESA delayed molting in premolt green morphs, whereas intact and MLA animals molted by 30 days post treatment. There were significant effects on YO gene expression in intact animals: Cm-GC-Iβ mRNA increased during premolt and Cm-GC-III mRNA decreased during premolt and increased during postmolt. Cm-MIH transcripts were detected in eyestalk ganglia, the brain and the thoracic ganglion from green intermolt animals, suggesing that MIH in the brain and thoracic ganglion prevents molt induction in green ESA animals.

The nuclear receptor E75 from the swimming crab, Portunus trituberculatus: cDNA cloning, transcriptional analysis, and putative roles on expression of ecdysteroid-related genes

“…The hemolymph 20E titer of P. trituberculatus increased from 1.78 ± 0.2 ng/mL to 4.20 ± 0.59 ng/mL at 2 d post ESA, and to 14.92 ± 8.50 ng/mL at 8 d post ESA (unpublished data in our group). Similar effects were also reported in several other crustaceans (Abuhagr et al, 2014;Chang et al, 1976;McDonald et al, 2011). In this study, the induced expression of PtE75, as well as that of PtEcR and PtRXR (Fig.…”

Effects of sex and color phase on ion regulation in the invasive European green crab, Carcinus maenas

“…To account for size variation effects, we used animals of similar size in experiments (mean carapace width ± SEM; green female 5.21 ± 0.08 cm, red female 5.23 ± 0.09 cm, green male 6.24 ± 0.19 cm, red male 6.29 ± 0.16 cm). We specifically selected animals of an intermediate size class as large animals (greater than 7 cm carapace width) often show signs of anecdysis (Webster 1986;Abuhagr et al 2014b). Only non-gravid females were used, since no gravid green phase were collected nor observed.…”

Effects of temperature on juvenile Dungeness crab,Metacarcinus magister(Dana): survival, moulting, and mTOR signalling and neuropeptide gene expression in eyestalk ganglia, moulting gland (Y-organ), and heart

“…Haemolymph samples were collected using needles and syringes (5 µl haemolymph immediately added to 50 µl dd H 2 O) and stored at −20°C at both time points. Haemolymph ecdysteroid concentration was quantified with an enzyme-linked immunosorbent assay (ELISA) modified from Kingan (1989) and Tamone et al (2007) as described in Abuhagr et al (2014a). At t 0 and t 14 , the basal endite of one of the third maxillipeds was excised and stored in crab saline and photographed under a microscope within 72 h to determine moult stage.…”