The relationship between carbon and nitrogen metabolism in cucumber leaves acclimated to salt stress

Naliwajski, Marcin; Skłodowska, Maria

doi:10.7717/peerj.6043

Cited by 32 publications

(29 citation statements)

References 90 publications

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“…Under salinity, the decrease in biomass observed in RIL-66 was correlated with an inhibition of all the photosynthetic and chlorophyll fluorescence parameters, in agreement with previous publications (Yu et al, 2010;Ashraf et al, 2018;Naliwajski and Skłodowska, 2018). However, the inhibition induced by the application of salinity+heat combined in this RIL-66 was observed only in the CO 2 assimilation index and in the maximum quantum yield efficiency rate (Fv´/Fm´), with the rest of the photosynthetic parameters measured being very similar to those obtained under control conditions.…”

Section: Growth and Photosynthesis Were Differently Affected In The Ssupporting

confidence: 92%

Using Tomato Recombinant Lines to Improve Plant Tolerance to Stress Combination Through a More Efficient Nitrogen Metabolism

et al. 2020

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The development of plant varieties with a better nitrogen use efficiency (NUE) is a means for modern agriculture to decrease environmental pollution due to an excess of nitrate and to maintain a sufficient net income. However, the optimum environmental conditions for agriculture will tend to be more adverse in the coming years, with increases in temperatures, water scarcity, and salinity being the most important productivity constrains for plants. NUE is inherently a complex trait, as each step, including N uptake, translocation, assimilation, and remobilization, is governed by multiple interacting genetic and environmental factors. In this study, two recombinant inbred lines (RIL-66 and RIL-76) from a cross between Solanum lycopersicum and Solanum pimpinellifoilum with different degree of tolerance to the combination of salinity and heat were subjected to a physiological, ionomic, amino acid profile, and gene expression study to better understand how nitrogen metabolism is affected in tolerant plants as compared to sensitive ones. The ionomics results showed a different profile between the two RILs, with K + and Mg 2+ being significantly lower in RIL-66 (low tolerant) as compared to RIL-76 (high tolerant) under salinity and heat combination. No differences were shown between the two RILs in N total content; however, N-NO 3 − was significantly higher in RIL-66, whereas N-N org was lower as compared to the other genotype, which could be correlated with its tolerance to the combination of salinity and heat. Total proteins and total amino acid concentration were significantly higher in RIL-76 as compared to the sensitive recombinant line under these conditions. Glutamate, but more importantly glutamine, was also highly synthesized and accumulated in RIL-76 under the combination of salinity and heat, which was in agreement with the upregulation of the nitrogen metabolism related transcripts studied (SlNR, SlNiR, SlGDH, SlGLT1, SlNRT1.2, SlAMT1, and SlAMT2). This study emphasized the importance of studying abiotic stress in combination and how recombinant material with different degrees of tolerance can be

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Section: Growth and Photosynthesis Were Differently Affected In The Ssupporting

confidence: 92%

Using Tomato Recombinant Lines to Improve Plant Tolerance to Stress Combination Through a More Efficient Nitrogen Metabolism

et al. 2020

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“…The accumulation of NH 4 + ions reflects reduced nitrogen assimilation and decreased plant growth (Wang et al, 2007). The salt-induced increase in NADH-GDH activity was previously reported from different crop plants (Kwinta & Cal, 2004; Wang et al, 2007; Gao et al, 2013; Naliwajski & Skłodowska, 2018).…”

Section: Discussionsupporting

confidence: 69%

Effects of salinity on photosynthetic traits, ion homeostasis and nitrogen metabolism in wild and cultivated soybean

Ullah

Noor

et al. 2019

PeerJ

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BackgroundCarbon and nitrogen metabolism need to be highly regulated to achieve cell acclimation to changing environmental conditions. The understanding of physio-biochemical responses of crops to salinity stress could help to stabilize their performance and yield. In this study we have analyzed the roles of photosynthesis, ion physiology and nitrate assimilation toward saline/alkaline stress acclimation in wild and cultivated soybean seedlings.MethodsGrowth and photosynthetic parameters, ion concentrations and the activity of enzymes involved in nitrogen assimilation were determined in seedlings of one wild and one cultivated soybean accession subjected to saline or alkaline stresses.ResultsBoth saline and alkaline stresses had a negative impact on the growth and metabolism of both wild and cultivated soybean.The growth, photosynthesis, and gas exchange parameters showed a significant decrease in response to increasing salt concentration. Additionally, a significant increase in root Na+ and Cl– concentration was observed. However, photosynthetic performance and ion regulation were higher in wild than in cultivated soybean under saline and alkaline stresses. Nitrate reductase (NR) and the glutamine synthetase/glutamate synthase (GS/GOGAT) cycle showed a significant decrease in leaves of both genotypes. The reduction in the GS/GOGAT cycle was accompanied by high aminating glutamate dehydrogenase (NADH-glutamate dehydrogenase) activity, indicating the assimilation of high levels of NH4+. A significant increase in the activities of aminating and deaminating enzymes, including glutamate dehydrogenase (GDH), alanine aminotransferase (AlaAT) and aspartate aminotransferase (AspAT), was observed, probably due to the high glutamate demand and maintenance of the Krebs cycle to correct the C: N status.ConclusionsCultivated soybean was much more stress sensitive than was the wild soybean. The decrease in growth, photosynthesis, ion regulation and nitrogen assimilation enzymes was greater in cultivated soybean than in wild soybean. The impact of alkaline stress was more pronounced than that of saline stress. Wild soybean regulated the physiological mechanisms of photosynthesis and nitrate assimilation more effectively than did cultivated soybean. The present findings provide a theoretical basis with which to screen and utilize wild and cultivated soybean germplasm for breeding new stress-tolerant soybean.

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“…The positive correlation, observed between carbohydrates, polyols, amino acids and organic acids might serve as third line ‘quick energy defense’ suggesting an anaplerotic role for the TCA cycle and provide immediate carbon energy source under salt stress (Zhang et al, 2017). The association between carbohydrates and amino acids showed maintain C:N balance, which may ameliorate salt-induced stress in Pongamia (Nasir et al, 2010;Naliwajski Sklodowska, 2018).…”

Section: Discussionmentioning

confidence: 99%

Systematic hormone-metabolite network provides insights of high salinity tolerance inPongamia pinnata(L.) pierre

Marriboina

Sharma

Sengupta

et al. 2020

Preprint

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Salinity stress results significant losses in plant productivity, and loss of cultivable lands. Although Pongamia pinnata is reported to be a salt tolerant semiarid tree crop, the adaptive mechanisms to saline environment are elusive. The present investigation describes alterations in hormonal and metabolic responses in correlation with physiological and molecular variations in leaves and roots of Pongamia at sea salinity level (3% NaCl) for 8 days. At physiological level, salinity induced adjustments in plant morphology, leaf gas exchange and ion accumulation patterns were observed. Our study also revealed that phytohormones including JAs and ABA play crucial role in promoting the salt adaptive strategies such as apoplasmic Na+ sequestration and cell wall lignification in leaves and roots of Pongamia. Correlation studies demonstrated that hormones including ABA, JAs and SA showed a positive interaction with selective compatible metabolites (sugars, polyols and organic acids) to aid in maintaining osmotic balance and conferring salt tolerance to Pongamia. At the molecular level, our data showed that differential expression of transporter genes as well as antioxidant genes regulate the ionic and ROS homeostasis in Pongamia. Collectively, these results shed new insights on an integrated physiological, structural, molecular and metabolic adaptations conferring salinity tolerance to Pongamia.High lightOur data, for the first time, provide new insights for an integrated molecular and metabolic adaptation conferring salinity tolerance in Pongamia. The present investigation describes alterations in hormonal and metabolic responses in correlation with physiological and molecular variations in Pongamia at sea salinity level (3% NaCl) for 8 days.

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The relationship between carbon and nitrogen metabolism in cucumber leaves acclimated to salt stress

Cited by 32 publications

References 90 publications

Using Tomato Recombinant Lines to Improve Plant Tolerance to Stress Combination Through a More Efficient Nitrogen Metabolism

Using Tomato Recombinant Lines to Improve Plant Tolerance to Stress Combination Through a More Efficient Nitrogen Metabolism

Effects of salinity on photosynthetic traits, ion homeostasis and nitrogen metabolism in wild and cultivated soybean

Systematic hormone-metabolite network provides insights of high salinity tolerance inPongamia pinnata(L.) pierre

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