The safe operation of military aircraft is crucial for national defense to prevent unnecessary loss of combat power. With the advancement of technology, the proportion of accidents caused by mechanical defects is decreasing compared to the beginning of aviation history, but the proportion of accidents caused by human factors is increasing. Therefore, it is necessary to identify factors that enhance flight safety behavior from various perspectives and explore related mechanisms. In this study, we examined the relationship between Air Force aircrew's calling and their flight safety behavior. With a sample of 357 Air Force aircrew, we found a significant positive relationship between calling and flight safety behavior, with affective commitment and moral duty mediating this relationship in parallel. We discussed theoretical and practical implications of the study based on these results.