The relationship between blastocyst morphology, chromosomal abnormality, and embryo gender

Alfarawati, S; Fragouli, Elpida; Colls, P.; Stevens, John; Gutiérrez-Mateo, Cristina; Schoolcraft, William B.; Katz‐Jaffe, Mandy G.; Wells, Dagan

doi:10.1016/j.fertnstert.2010.04.003

Cited by 344 publications

(260 citation statements)

References 34 publications

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“…Aneuploidy in IVF embryos is relatively common (Alfarawati et al, 2011;Angell et al, 1983) and, as shown here, also occurs in PBNT blastocysts. Aneuploidy rates in human embryos increase with maternal age as a result of meiosis I or II errors (Hassold and Hunt, 2001;Nagaoka et al, 2012;Petronczki et al, 2003).…”

Section: Discussionsupporting

confidence: 75%

“…In the control group, 75% (12/16) of the zygotes reached blastocysts while only 42% (8/19) of PBNT zygotes did (Student's t-test, p < 0.05, Figure 1F, 1G and Table S2). Since embryonic aneuploidy resulting from numerical chromosomal abnormalities leading to implantation failure or spontaneous pregnancy loss is common irrespective of blastocyst morphology (Alfarawati et al, 2011), we subjected selected PBNT (n=2) and control (n=3) blastocysts to short tandem repeat (STR) analysis. Examination of microsatellite markers mapping to 22 human autosomal loci and one X-linked locus revealed that two sampled PBNT blastocysts contained normal diploid chromosomes contributed from both oocyte and sperm.…”

Section: Preimplantation Development and Ploidy Of Pbnt Blastocystsmentioning

confidence: 99%

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Functional Human Oocytes Generated by Transfer of Polar Body Genomes

O’Neil

Gutiérrez

et al. 2017

Cell Stem Cell

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Section: Discussionsupporting

confidence: 75%

Section: Preimplantation Development and Ploidy Of Pbnt Blastocystsmentioning

confidence: 99%

Functional Human Oocytes Generated by Transfer of Polar Body Genomes

O’Neil

Gutiérrez

et al. 2017

Cell Stem Cell

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“…New genetic laboratory techniques for preimplantation genetic screening deserve to be tested in the same rigorous way as the older (PCR, FISH) ones. The results so far suggest that a consistent proportion of aneuploid embryos arrest their growth at some stage of development, even after blastocyst formation [75].…”

Section: Preimplantation Screeningmentioning

confidence: 86%

Birth defects and congenital health risks in children conceived through assisted reproduction technology (ART): a meeting report

Albertini

Evers²,

Geraedts³

et al. 2014

J Assist Reprod Genet

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Purpose Assisted Reproduction Treatment (ART) is here to stay. This review addresses the parental background of birth defects, before, during and after conception and focuses both on the underlying subfertility and on the question whether ART as a treatment is an additional contributing factor. Methods Searches were performed in Medline and other databases. Summaries were discussed in a Delphi panel set-up by the European Society of Human Reproduction and Embryology (ESHRE). Results Several birth defects and adult diseases arise during the earliest stages of ovarian development and oocyte differentiation: this is the case of cleft palate disorders in offspring from female rat exposed to Dioxin during fetal life or the polycystic ovary diseases in female offspring (primates) exposed to elevated androgen concentration during fetal life. Human oocytes and embryos often fail to stop the propagation of aneuploid cells but maintain their ability to repair DNA damages including those introduced by the fertilizing sperm. There is a 29 % increased risk of birth defects in the newborns spontaneously conceived by subfertile couples and the risk is further increased (34 %) when conception is achieved by treating infertlity with ART (Danish IVF Registry). Periconceptional conditions are critical for ART babies: their birth weight is in general smaller (Norvegian Registry) but a more prolonged culture time doubled the number of large babies (Finnish Registry). Conclusion The long-term developmental effects of ART on child and subsequent health as an adult remains a subject worthy of futher monitoring and investigation. Capsule Remaining mindful of the growing body of evidence showing that during gamete production, genetic and epigenetic changes influence offspring health, serves to reinforce the need to continue to monitor ART children.

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“…However, at the blastocyst stage abnormalities were overrepresented amongst embryos considered to be of poor morphology. Other research based on trophectoderm biopsy to perform chromosomal analysis has shown higher aneuploidy rates in slower developing blastocysts [27]. These studies demonstrate that there is an association between chromosomal abnormality and embryo development and that there is progressive loss of chromosomally abnormal embryos during preimplantation development [25].…”

Section: Discussionmentioning

confidence: 62%

The clinical outcomes of day 3 4-cell embryos after extended in vitro culture

Zhao

Lian

et al. 2014

J Assist Reprod Genet

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Objective To evaluate the development potential and clinical significance of day 3 4-cell embryos after extended in vitro culture. Methods This study was a retrospective cohort study for patients with infertility treatment between January 2011 and July 2013. Patients undergoing blastocyst culture in controlled ovarian hyperstimulation cycles using surplus embryos were analyzed in the study. A total of 764 women undergoing blastocyst culture with 1,522 surplus 4-cell embryos on day 3 were analyzed. An additional 2,391 patients with embryos undergoing blastocyst culture during the same period with embryos having more blastomeres were chosen as control. Results After extended culture, 253 embryos from 183 cycles in the study group which developed to blastocysts were frozen, and 118 embryos were warmed in subsequent frozen embryo transfer cycles. Implantation rates, clinical pregnancy rates (PRs) and ongoing PRs were 33.3 %, 38.4 % and 31.4 %, respectively, which were similar to those of the control group. The singleton birth weights of newborns using these blastocysts showed no significant difference to that seen in the control group. Conclusion Surplus 4-cell embryos on day 3 displayed lower blastulation rates. However, once a blastocyst is obtained, it has equivalent clinical outcomes. Embryos that are developmentally lagging on day 3 can be observed in extended culture to increase the cumulative chances of a successful pregnancy.

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The relationship between blastocyst morphology, chromosomal abnormality, and embryo gender

Cited by 344 publications

References 34 publications

Functional Human Oocytes Generated by Transfer of Polar Body Genomes

Functional Human Oocytes Generated by Transfer of Polar Body Genomes

Birth defects and congenital health risks in children conceived through assisted reproduction technology (ART): a meeting report

The clinical outcomes of day 3 4-cell embryos after extended in vitro culture

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