The relationship between 174 G/C and -572 G/C of IL-6 gene polymorphisms and susceptibility of celiac disease in the Iranian population

Abstract

IntroductionCeliac disease (CD) is a chronic inflammatory intestinal disorder. Different immunological factors, including inflammatory cytokines, may play an important role in disease susceptibility.AimTo investigate the relationship between -174G/C and -572G/C gene polymorphisms and the serum level of interleukin 6 (IL-6) and susceptibility to CD in the Iranian population.Material and methodsIn this case-control study blood samples were collected of 105 patients with CD and 106 healthy subjects randomly in 2016 and evaluated by polymerase chain reaction-restriction fragments length polymorphism (PCR-RFLP) method. A sequence was also used to confirm the results of both polymorphisms. The IL-6 concentration was measured using ELISA.ResultsThe results showed a significant relationship between polymorphism -572G in CD patients when compared with control subjects by genotype (p = 0.001) and alleles (p = 0.022), respectively. There was no significant relationship between polymorphism 174G and frequency of genotype, but an association of this polymorphism with the frequency of alleles (p = 0.034), age (p = 0.001), and body mass index (p = 0.003) was seen. The serum level of interleukin-6 was significantly associated only with rs1800796 (p < 0.001).ConclusionsThe results confirm previous studies in different parts of the world and indicate that IL-6 (572G/C) polymorphism may play a role in susceptibility to CD in the Iranian population.

ASSOCIATIONS OF IL-6 -174G>C AND IL-10 -1082A>G POLYMORPHISMS WITH SUSCEPTIBILITY TO CELIAC DISEASE: EVIDENCE FROM A META-ANALYSIS AND LITERATURE REVIEW

“…Dema et al, have reported that the IL-6 -174G>C polymorphism was significantly associated with increased risk of CD susceptibility in Spanish girls (17) . However, Barartabar et al, not found a significant association between IL-6 -174G>C polymorphism and increased risk of CD in an Iranian population (19) . This inconsistency could result from relatively small sample size, from genetic ethnical heterogeneity or from unknown local environmental factors.…”

“…Afterward, 19 studies that focused on other SNPs rather than IL-6 -174G>C and IL-10 -1082A>G polymorphisms, review articles, case only studies and studies with insufficient data polymorphism were excluded. Finally, a total of nine case-control studies involving five studies with 737 cases and 1,338 control on IL-6 -174G>C polymorphism (15)(16)(17)(18)(19) , and four studies with 923 cases and 864 controls on IL-10 -1082A>G polymorphism (16,(20)(21)(22) were selected. The Characteristics of included studies are shown in TABLE 1.…”

“…Celiac disease (CD), also called gluten intolerance, is a chronic and immune-mediated enteropathy with intestinal and extraintestinal manifestations triggered by the ingestion of gluten-containing grains in genetically predisposed individuals (1)(2)(3) . A review of celiac disease studies showed a biopsy-proven prevalence ranging from 0.15% to 1.9% in unselected populations of North America and Western Europe (4) .…”

Association of Genetic Polymorphisms and Serum Levels of IL-6 and IL-8 with the Prognosis in Children with Neuroblastoma

“…Several studies have shown that the −174 IL-6 (G > C) rs1800795 polymorphism produces an alteration in transcriptional activity that can result in a functional change that affects IL-6 serum levels. Previous studies have identified significant elevations of serum IL-6 levels in patients who develop various diseases, such as rheumatoid arthritis; Crohn’s disease; and some types of cancer, such as lymphomas, chronic lymphocytic leukemias, prostate cancer, breast cancer, gastric cancer, and neuroblastoma [ 50 , 51 , 52 , 53 , 54 ]. In this study, regarding the association between the serum levels of this IL-6 with the clinical characteristics of the patients, it was identified that 40.7% of the patients with low levels were <18 months of age.…”

The Genetic Aspects of Behçet’s Disease: Role of Cytokine Genes Polymorphisms

“…IL-6 pleitropic cytokine is involved in immune and inflammatory responses. Various polymorphisms in IL-6 gene have been associated with chronic inflammatory and autoimmune disorders [84][85][86][87][88]. Higher levels of IL-6 and increased expression of IL-6 mRNA have been reported in subjects with active BD [20,89,90].…”