The Relation of Allergy Reagins to Electrophoretic Components of Serum

The Antibody Mechanism of Ragweed Allergy. Electrophoretic and Chemical Studies

“…Convection electrophoresis. This technique has also been ap plied to ascertain the association of sk-antibody with electropho retic serum components (11,12,13,14,15). We refer to data of Cann and Loveless (14), from which they conclude that skantibody is predominantly associated with beta globulin, on the grounds of a seeming parallelism between dilutability of fractions and their beta globulin content.…”

“…Some results of Campbell's (11,13) which indicate association of sk-antibody with alpha2 globulin arc based on Convection Elec trophoresis experiments, where the pH values of the medium were not lowered stepwise as was done by Cann and Loveless. Although Campbell does not report on his reactivity recoveries, he un questionably obtained from a ragweed sensitive serum reactive * The cathodic and anodic endvcsscls no.…”

The Localization of Skin-Sensitizing Antibody in the Sera of Ragweed-Sensitive Individuals by Electrophoresis

“…In a few instances, however, in the papers referred to in the introduction, reaginic activity was found in fractions which contained no ^/-globulin (5,8). There are two possible explanations.…”

Studies on the Atopic Allergen in Hen’s Egg

“…R a p p a p o r t [21] for example studied reagin-allergcn combinations in skin sites from eggsensitive patients who were challenged with ovalbumin. C a m p b e l l [10] also used ovalbumin preparations for the identification of the reagin fraction in the sera of patients with positive direct and P r a u s n it z -K u s t n e r reactions to egg white. Moreover ovalbumin is sometimes routinely used for skin test purposes [23].…”