The regulation of hepatic tyrosine aminotransferase

Evans, Peter

doi:10.1016/0304-4165(81)90257-9

Cited by 32 publications

(11 citation statements)

References 34 publications

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“…Various reports indicate that GR has been involved in regulation of gene expression for several key metabolic enzymes including TAT (Evans 1981) and TO (DeLap and Feigelson 1978). Thus, TAT and TO genes provide good model system to investigate related changes in GR mediated gene expression in vivo in whole organism.…”

Section: Introductionmentioning

confidence: 98%

Basal and glucocorticoid induced changes of hepatic glucocorticoid receptor during aging: relation to activities of tyrosine aminotransferase and tryptophan oxygenase

Ribarac-Stepic¹,

Vulovic²,

Korićanac³

et al. 2005

Biogerontology

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The characteristics of glucocorticoid receptors, their sensitivity to glucocorticoid as well as the basal and glucocorticoid induced thyrosine aminotranferase (TAT) and tryptophan oxygenase (TO) activities were studied in rat liver during aging. The concentration (N) and dissociation constant (K(d)) of glucocorticoid receptor (GR) significantly change during the aging both in untreated and dexamethasone treated animals. The level of receptors was lower in dexamethasone treated rats of all analyzed aged groups compared to untreated animals. In comparison to untreated groups, there was no correlation between the changes of N and K(d) during the lifespan. According to immunochemical analysis, the decline of receptor protein content occurs during lifespan. Dexamethasone treatment reduced the level of receptor protein compare to respective age group of untreated rats. The glucocorticoid-receptor (G-R) complexes from both untreated and treated animals underwent thermal activation, although the extent of activation was more pronounced in the case of untreated groups compared to treated animals. The magnitude of heat activation of receptor complexes was more pronounced in the liver of the youngest untreated rats compared to elderly ones, while the receptor activation between treated groups of studied ages has shown less significant differences. Besides, basal as well as induced TAT and TO activities after dexamethasone injection also showed age-related alterations. The observed alterations in GR might play a role in the changes of the cell responses to glucocorticoid during the age. This presumption is supported by detected changes in basal and dexamethasone induced TAT and TO activities during aging.

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Section: Introductionmentioning

confidence: 98%

Basal and glucocorticoid induced changes of hepatic glucocorticoid receptor during aging: relation to activities of tyrosine aminotransferase and tryptophan oxygenase

Ribarac-Stepic¹,

Vulovic²,

Korićanac³

et al. 2005

Biogerontology

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“…The time course for enzyme induction remained the same at all preservation times. It involves hormone receptor binding, occupied receptor translocation, transcription and translation (Evans, 1981). A rapid, cell type specific process has been maintained in this preservation system.…”

Section: Resultsmentioning

confidence: 99%

“…Rat hepatocytes were prepared as described previously (Evans, 1981). The cells were plated on collagen coated dishes (60 mm) or Primaria plates (Falcon).…”

Section: Preparation and Preservation Of Hepatocytesmentioning

confidence: 99%

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Preservation and Transportation of Hepatocyte Monolayers in A No Spill Format at Non Freezing Temperatures

Evans¹

2014

IJB

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Hepatocyte monolayers were preserved under a gelatin overlay at 10 C. Morphological changes were observed after 4 days, as structure less areas at the cell periphery. The changes could be prevented by adding sucrose (0.05M) to the gel. Cell viability remained high for 7 days. Cell spreading occurred rapidly when the cells were reactivated. Basal enzyme levels were maintained during the preservation period. The hormonal induction of tyrosine aminotransferase was stable for 4 days but declined subsequently. Sucrose addition extended the period of preservation where induction was evident. The method provides a robust system that allows preservation in the absence of potentially deleterious chemicals. Time is allowed to send cells anywhere in the World in a no spill format whist arriving at their destination as if freshly produced.

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“…Gels were scanned at 530 nm. En zyme assays were as described by Evans [5], Heat Treatment. The pellet was redissolved in 20 mmol/1 potassium phosphate pH 6.6 containing 1 mmol/1 mercaptoethanol, 1 mmol/1 EDTA, 2 mmol/1 2-oxoglutarate and 0.2 mmol/1 pyridoxal-5'-phosphate and heated at 60 °C for 5 min.…”

Section: Sephacryl S-200mentioning

confidence: 99%

Purification and Electrophoretic Analysis of Rabbit Cytosolic Tyrosine Aminotransferase

Evans¹

1982

Enzyme

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Tyrosine aminotransferase from rabbit liver was purified 3,000-fold to electrophoretic homogeneity. Subforms of tyrosine aminotransferase were not observed by enzymic analysis. Aspartate aminotransferase did not utilise tyrosine. Five bands of apparent tyrosine aminotransferase were detected by histochemical staining of polyacrylamide gels but detailed study showed that only one represented true tyrosine aminotransferase activity.

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The regulation of hepatic tyrosine aminotransferase

Cited by 32 publications

References 34 publications

Basal and glucocorticoid induced changes of hepatic glucocorticoid receptor during aging: relation to activities of tyrosine aminotransferase and tryptophan oxygenase

Basal and glucocorticoid induced changes of hepatic glucocorticoid receptor during aging: relation to activities of tyrosine aminotransferase and tryptophan oxygenase

Preservation and Transportation of Hepatocyte Monolayers in A No Spill Format at Non Freezing Temperatures

Purification and Electrophoretic Analysis of Rabbit Cytosolic Tyrosine Aminotransferase

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