Abstract:Hepatocyte monolayers were preserved under a gelatin overlay at 10 C. Morphological changes were observed after 4 days, as structure less areas at the cell periphery. The changes could be prevented by adding sucrose (0.05M) to the gel. Cell viability remained high for 7 days. Cell spreading occurred rapidly when the cells were reactivated. Basal enzyme levels were maintained during the preservation period. The hormonal induction of tyrosine aminotransferase was stable for 4 days but declined subsequently. Suc… Show more
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