The regulation of cell growth I. Identification and partial characterization of a DNA synthesis stimulating factor from the serum of partially hepatectomized rats

Morley, Colin G.D.; Kingdon, Henry S.

doi:10.1016/0005-2787(73)90156-1

Cited by 71 publications

(19 citation statements)

References 21 publications

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“…One has a molecular weight of over 120 KD is and heat-labile, while the other is less than 3 KD and is heat-stable. Morley and Kingdon also reported the presence of a hepatocyte growth factor in the serum of rats after partial hepatectomy (20). This factor has a molecular weight of 26 KD and is heat-stable.…”

Section: IImentioning

confidence: 91%

Control of growth and expression of differentiated functions of mature hepatocytes in primary culture.

Nakamura

Ichihara

1985

Cell Struct. Funct.

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Section: IImentioning

confidence: 91%

Control of growth and expression of differentiated functions of mature hepatocytes in primary culture.

Nakamura

Ichihara

1985

Cell Struct. Funct.

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“…Various fractions of liver have been employed in many experiments without consistent results and with varying degrees of success (Bucher, 1963;Kelly & Jones, 1953;Leduc, 1964;Morley & Kingdon, 1973;Paschkis, 1958). Earlier work by one of the authors (D. L.) suggested that liver itself might be the site of production of these growth promoting factor(s) (LaBrecque, 1965).…”

Section: R Labrecque and L A Peschmentioning

confidence: 99%

Preparation and partial characterization of hepatic regenerative stimulator substance (SS) from rat liver.

LaBrecque¹,

Pesch²

1975

The Journal of Physiology

170

114

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1. The elucidation of the biochemical controls of growth processes is basic to understanding both normal and malignant growth. The exquisitely sensitive control demonstrated in the regenerating liver provides an excellent model system for growth studies. 2. Intraperitoneal injection of an extract from weanling rat liver into 34% hepatectomized rats stimulates [3H]thymidine incorporation into recipient hepatic DNA 2.5‐fold. This stimulation is seen after a 10 hr lag period. 3. Extracts from weanling rat liver contain the highest level of the hepatic regenerative stimulator substance (SS), whereas extracts from adult liver give no significant stimulation. 4. If adult rats are partially hepatectomized, SS is demonstrated in their livers 12–15 hr after hepatectomy. 5. Partial characterization of SS shows that it is a soluble material not sedimented by centrifugation for 2 hr at 145,000 g. The stimulator activity is stable to heating at 65 degrees C and 100 degrees C for 15 min but is lost when treated with 10% perchloric acid.

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“…In this context, some authors have reported the existence of liver growth factors (Morley & Kingdon, 1973;Russell et al, 1984;Nakamura et al, 1984). The possible relationship of any of these factors to the HP purified by our group is unknown at present, and equally unknown is the importance of these growth factors in the regenerative process studied in this work.…”

Section: Discussionmentioning

confidence: 75%

A liver DNA synthesis promoter induced in rat plasma by injection of dimethylnitrosamine (DMNA) or thioacetamide

Díaz-Gil¹,

Sánchez²,

Santamarı́a³

et al. 1987

Br J Cancer

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Summary The appearance of a liver DNA synthesis promoter (HP) in rat plasma after dimethylnitrosamine (DMNA) or thioacetamide injection was investigated. After 48 h, DMNA (30mg kg-1 body weight) produced liver (centrilobular) necrosis and intense hepatic regeneration, as assessed by microscopic observations of liver slices, as well as augmented transaminase levels; HP was detectable under these conditions. After 5 days, transaminases and HP returned to normal values (the latter undetectable), coinciding with a lack of necrotic zones. At 60mg DMNA kg-' body weight, necrotic areas were more marked and transaminases and HP levels higher after 48 h than with the lower dose; these increases were even more pronounced at 90mg DMNA kg-1 body weight.After thioacetamide injection (200mgkg-1 body wt) the situation at 48h was very similar, with focal, centrilobular necrosis, frequent regenerative signs, high transaminases and detectable HP. Rats recovered after 7 days in a similar fashion as with DMNA. At 400mg thioacetamide kg-1 body weight, necrotic areas and regeneration zones were more widespread and transaminases and HP higher after 48h than with the lower dose.On account of the differing modes of action of DMNA and thioacetamide in rat liver, it is proposed that the appearance of HP activity in plasma could be related to the regenerative process that follows hepatotoxic damage.

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The regulation of cell growth I. Identification and partial characterization of a DNA synthesis stimulating factor from the serum of partially hepatectomized rats

Cited by 71 publications

References 21 publications

Control of growth and expression of differentiated functions of mature hepatocytes in primary culture.

Control of growth and expression of differentiated functions of mature hepatocytes in primary culture.

Preparation and partial characterization of hepatic regenerative stimulator substance (SS) from rat liver.

A liver DNA synthesis promoter induced in rat plasma by injection of dimethylnitrosamine (DMNA) or thioacetamide

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