The Regulation Network and Clinical Significance of Circular RNAs in Breast Cancer

Xu, Juan; Chen, Xiyi; Sun, Yu; Shi, Yaqian; Fang, Teng; Lv, Mingming; Liu, Chen; Jia, Xuemei

doi:10.3389/fonc.2021.691317

Cited by 3 publications

(1 citation statement)

References 178 publications

(107 reference statements)

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“…Most studies have proven that circRNAs act as sponges of microRNAs (miRNAs) to further regulate the development of multiple human diseases [ 8 , 12 – 16 ]. In human BC, many circRNAs have been discovered and proven to be related to the development of BC [ 17 ]. Notably, a meta-analysis indicated that circRNAs have diagnostic value and may be potential diagnostic biomarkers for BC [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Circular RNA hsa_circ_0043278 inhibits breast cancer progression via the miR-455-3p/EI24 signalling pathway

Shi

Liu

2021

BMC Cancer

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Background Breast cancer (BC) is one of the major malignancies worldwide. Circular ribonucleic acids (circRNAs) are a class of conserved ribonucleic acid (RNA) molecules that play important roles in various diseases. Recently, circRNAs have been suggested to have diagnostic value and may function as potential diagnostic biomarkers for BC. Previously, hsa_circ_0043278 was found to be downregulated in human BC. However, its role in human BC has not yet been identified. Methods The levels of hsa_circ_0043278 in BC cell lines were verified by quantitative reverse transcription–polymerase chain reaction (qRT–PCR). The overexpression vector and short hairpin RNA (shRNA) of hsa_circ_0043278 were transfected into MDA-MB-231 and MCF-7 cells, respectively. The effects of hsa_circ_0043278 on tumour cell growth, migration and invasion were measured by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT), colony formation, wound healing and Transwell assays in vitro. A xenograft experiment was conducted to validate the inhibitory effect of hsa_circ_0043278 on tumour growth. The interaction between hsa_circ_0043278 and miR-455-3p was confirmed by a dual-luciferase reporter assay. Mimics and inhibitors of miR-455-3p were designed to confirm the influence of hsa_circ_0043278 on the hsa_circ_0043278/miR-455-3p/etoposide-induced gene 24 (EI24) axis. Results Hsa_circ_0043278 was downregulated in BC cell lines. Furthermore, overexpression of hsa_circ_0043278 notably decreased BC cell viability and inhibited BC cell migration and invasion in vitro and suppressed tumour growth in vivo. Downregulation of hsa_circ_0043278 led to the opposite results. Hsa_circ_0043278 expression was negatively correlated with that of miR-455-3p. In addition, mechanistic investigation proved that hsa_circ_0043278 directly bound to miR-455-3p and regulated EI24 and NF-κB expression in BC cells. Conclusion Hsa_circ_0043278 acts as a tumour suppressor gene in BC through the hsa_circ_0043278/miR-455-3p/EI24 axis and may be regarded as a new prognostic predictor or potential therapeutic target in BC.

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Section: Introductionmentioning

confidence: 99%

Circular RNA hsa_circ_0043278 inhibits breast cancer progression via the miR-455-3p/EI24 signalling pathway

Shi

Liu

2021

BMC Cancer

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Circular RNAs: New layer of complexity evading breast cancer heterogeneity

Dawoud

Ihab²,

Hisham³

et al. 2023

Non-coding RNA Research

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Circular RNAs as Potential Biomarkers in Breast Cancer

et al. 2022

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Due to the high heterogeneity and initially asymptomatic nature of breast cancer (BC), the management of this disease depends on imaging together with immunohistochemical and molecular evaluations. These tests allow early detection of BC and patient stratification as they guide clinicians in prognostication and treatment decision-making. Circular RNAs (circRNAs) represent a class of newly identified long non-coding RNAs. These molecules have been described as key regulators of breast carcinogenesis and progression. Moreover, circRNAs play a role in drug resistance and are associated with clinicopathological features in BC. Accumulating evidence reveals a clinical interest in deregulated circRNAs as diagnostic, prognostic and predictive biomarkers. Furthermore, due to their covalently closed structure, circRNAs are highly stable and easily detectable in body fluids, making them ideal candidates for use as non-invasive biomarkers. Herein, we provide an overview of the biogenesis and pleiotropic functions of circRNAs, and report on their clinical relevance in BC.

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The Regulation Network and Clinical Significance of Circular RNAs in Breast Cancer

Cited by 3 publications

References 178 publications

Circular RNA hsa_circ_0043278 inhibits breast cancer progression via the miR-455-3p/EI24 signalling pathway

Circular RNA hsa_circ_0043278 inhibits breast cancer progression via the miR-455-3p/EI24 signalling pathway

Circular RNAs: New layer of complexity evading breast cancer heterogeneity

Circular RNAs as Potential Biomarkers in Breast Cancer

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