The receptor function of the Na+, K+-activated adenosine triphosphatase system

Anner, Béatrice M.

doi:10.1042/bj2270001

Cited by 68 publications

(15 citation statements)

References 127 publications

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“…24 The ouabain was used as an inhibitor of ATPase, because it could work as an endogenous highly specific Na ＋ -K ＋ -ATPase inhibitor. 25,26 As expected, Figure 6 shows that ouabain inhibits the Rb ＋ influx significantly, which proved that Rb ＋ has the same pathway as K ＋ . Rb ＋ was assumed to be able to take the binding point of Na ＋ -K ＋ -ATPase cross the membrane instead of K ＋ .…”

supporting

confidence: 60%

Rb+ Uptake by Human Erythrocytes and Its Transmembrane Pathway

Jiang

et al. 2006

Chin. J. Chem.

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The erythrocyte hemolysis was examined to assure Rb ＋ to have a weak toxicity toward human body. The way of Rb ＋ transporting into human erythrocytes was determined, and the factors to affect this transport process were evaluated. The effects of extracellular concentration of Rb ＋ , incubation temperature, incubation time, medium pH, and specific inhibitors were investigated via flame atomic absorption spectrometry. The results indicated that the membrane transport of Rb ＋ through human erythrocytes was controlled mainly by both active transport and simple diffusion. Every mentioned factor took a positive effect on the Rb ＋ uptake by human erythrocytes, however neither DIDS nor nefidpine could inhibit the uptake of Rb ＋ .

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confidence: 60%

Rb+ Uptake by Human Erythrocytes and Its Transmembrane Pathway

Jiang

et al. 2006

Chin. J. Chem.

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“…33,34 Some of this controversy relates to the uncertainty regarding which subtypes are expressed in the human liver. Although there is general agreement on the presence of the ␣1 subunit, the status of the ␤1 subunit is less clear.…”

Section: Discussionmentioning

confidence: 99%

Decreased hepatocyte membrane potential differences and GABAa-β3 expression in human hepatocellular carcinoma

et al. 2007

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To determine whether hepatocyte membrane potential differences (PDs) are depolarized in human HCC and whether depolarization is associated with changes in GABA A receptor expression, hepatocyte PDs and ␥-aminobutyric acid (GABA) A receptor messenger RNA (mRNA) and protein expression were documented in HCC tissues via microelectrode impalement, real-time reverse-transcriptase polymerase chain reaction, and Western blot analysis, respectively. HCC tissues were significantly depolarized (؊19.8 ؎ 1.3 versus ؊25. In recent in vitro studies, we demonstrated that restoration of depolarized malignant hepatocyte cell membrane potential differences (PDs) toward those documented in nonmalignant hepatocytes results in a loss of malignant features, including decreased proliferative activity, absence of colony formation in soft agar, and normalization of phenotypic appearance. 2 Whether HCC tissues are depolarized relative to adjacent nontumor tissues and the mechanisms whereby such depolarization might exist have yet to be reported.

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“…50, 12 15-12 19 ( 1988). phorylation sites on the enzyme (Erdmann and Schoner, 1973;Anner, 1985). Thus, quantitation of [3H]ouabain binding gives a measure of the number of (Na+ + K+)-ATPase molecules present in'a tissue.…”

mentioning

confidence: 99%

Effect of Fatty Acids on [³H]Ouabain Binding to Cerebromicrovascular (Na⁺+ K⁺)‐ATPase

Caspers

Grammas

1988

Journal of Neurochemistry

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The effects of short- and long-chain fatty acids on the cerebromicrovascular (Na+ + K+)-ATPase were investigated using specific [3H]ouabain binding to the enzyme. Specific binding increased linearly with total microvessel protein (37-110 micrograms) and was time-dependent with maximum binding obtained by 10 min. Arachidonic acid, but not palmitic acid, stimulated [3H]ouabain binding in a dose-dependent manner, with a 105% increase over basal levels at 100 microM arachidonic acid. Preincubation of the microvessels with arachidonic acid did not alter the stimulation observed. 4-Pentenoic acid stimulated [3H]ouabain binding only at high concentrations (10 mM). Scatchard analysis of [3H]ouabain binding to untreated microvessels yielded a single class of "high-affinity" binding sites with an apparent binding affinity (KD) of 64.7 +/- 2.0 nM and a binding capacity (Bmax) of 10.1 +/- 1.5 pmol/mg protein. In the presence of 100 microM arachidonic acid, a monophasic Scatchard plot also was obtained, but the KD significantly decreased to 51.9 +/- 2.7 nM (p less than 0.01), whereas the Bmax remained virtually unchanged (12.5 +/- 1.2 pmol/mg protein). The stimulation of [3H]ouabain binding in the presence of arachidonic acid was potentiated by 4-pentenoic acid, but not by indomethacin or eicosatetraynoic acid. These data suggest that long-chain polyunsaturated fatty acids may be involved in the regulation of blood-brain barrier (Na+ + K+)-ATPase and may play a role in the cerebral dysfunction associated with diseases in which plasma levels of nonesterified fatty acids are elevated.

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The receptor function of the Na+, K+-activated adenosine triphosphatase system

Cited by 68 publications

References 127 publications

Rb+ Uptake by Human Erythrocytes and Its Transmembrane Pathway

Rb+ Uptake by Human Erythrocytes and Its Transmembrane Pathway

Decreased hepatocyte membrane potential differences and GABAa-β3 expression in human hepatocellular carcinoma

Effect of Fatty Acids on [³H]Ouabain Binding to Cerebromicrovascular (Na⁺+ K⁺)‐ATPase

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The receptor function of the Na+, K+-activated adenosine triphosphatase system

Cited by 68 publications

References 127 publications

Rb+ Uptake by Human Erythrocytes and Its Transmembrane Pathway

Rb+ Uptake by Human Erythrocytes and Its Transmembrane Pathway

Decreased hepatocyte membrane potential differences and GABAa-β3 expression in human hepatocellular carcinoma

Effect of Fatty Acids on [3H]Ouabain Binding to Cerebromicrovascular (Na++ K+)‐ATPase

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Effect of Fatty Acids on [³H]Ouabain Binding to Cerebromicrovascular (Na⁺+ K⁺)‐ATPase