The Reaggregation of Dissociated Sponge Cells

Spiegel, Melvin

doi:10.1111/j.1749-6632.1955.tb40089.x

Cited by 33 publications

(6 citation statements)

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“…Since the pioneer work of Wilson () many studies dealt with sponge cell reaggregation. These studies had different aims and investigated diverse aspects of reaggregation process: molecular basis of cell reaggregation (Spiegel, ; Humphreys, , ; Müller and Müller, ; Fernàndez‐Busquets, ), self/non‐self recognition (Wilson, ; Galtsoff, , ; Spiegel, ; Curtis, ; Humphreys, , ; Van de Vyver, ; Leith, ; Custodio et al, ), cell dedifferentiation/transdifferentiation (Efremova, ; Bagby, ; Korotkova and Sokolova, ; Nikitin, ; Buscema et al, ; Volkova and Zolotareva, ), cell behavior in suspension and during early stages of reaggregation (Galtsoff, , ; Efremova and Drozdov, ; Noble and Peterson, ; Gaino et al, ; Gaino and Magnino, ), intact sponge regeneration from multicellular aggregates (Wilson, , ; Huxley, ; Galtsoff, ; Efremova, , ; Korotkova, ; Eerkes‐Medrano et al, ), physiological properties of multicellular aggregates (Custodio et al, ; Krasko et al, ; Le Pennec et al, ; Zhang et al, ; Müller et al, ; Cao et al, ,; Valisano et al, ; Chernogor et al, ), and multicellular aggregate potential for obtaining sponge cell cultures for biotechnological purposes (Müller et al, , ; Sipkema, ). Nevertheless, many details of sponge cell reaggregation still remain unclear (Lavrov and Kosevich, ) including questions of cell behavior and aggregates structure changes in the course of reaggregation.…”

Section: Discussionmentioning

confidence: 99%

Sponge cell reaggregation: Cellular structure and morphogenetic potencies of multicellular aggregates

Lavrov

Kosevich

2016

J. Exp. Zool.

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Sponges (phylum Porifera) are one of the most ancient extant multicellular animals and can provide valuable insights into origin and early evolution of Metazoa. High plasticity of cell differentiations and anatomical structure is characteristic feature of sponges. Present study deals with sponge cell reaggregation after dissociation as the most outstanding case of sponge plasticity. Dynamic of cell reaggregation and structure of multicellular aggregates of three demosponge species (Halichondria panicea (Pallas, 1766), Haliclona aquaeductus (Sсhmidt, 1862), and Halisarca dujardinii Johnston, 1842) were studied. Sponge tissue dissociation was performed mechanically. Resulting cell suspensions were cultured at 8-10°C for at least 5 days. Structure of multicellular aggregates was studied by light, transmission and scanning electron microscopy. Studied species share common stages of cell reaggregation-primary multicellular aggregates, early-stage primmorphs and primmorphs, but the rate of reaggregation varies considerably among species. Only cells of H. dujardinii are able to reconstruct functional and viable sponge after primmorphs formation. Sponge reconstruction in this species occurs due to active cell locomotion. Development of H. aquaeductus and H. panicea cells ceases at the stages of early primmorphs and primmorphs, respectively. Development of aggregates of these species is most likely arrested due to immobility of the majority of cells inside them. However, the inability of certain sponge species to reconstruct functional and viable individuals during cell reaggregation may be not a permanent species-specific characteristic, but depends on various factors, including the stage of the life cycle and experimental conditions.

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Section: Discussionmentioning

confidence: 99%

Sponge cell reaggregation: Cellular structure and morphogenetic potencies of multicellular aggregates

Lavrov

Kosevich

2016

J. Exp. Zool.

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“…McClay (1974) has investigated aggregation factors from five demo sponge species and has found that each of them only enhances the aggregation of homologous cells, that is, each factor is species specific. Initially, Spiegel (1955) showed that specific antisera prepared against Microciona prolifera cells inhibits Micro-ciona aggregation but not that of Cliona celata; while anti-Cliona serum inhibits Cliona reaggregation but not that of Microciona. Later, Moscona (1968) found that Microciona prolifera and Haliclona oculata aggregation factors are species specific in stimulating aggregation and that antibodies to Microciona factor precipitate and inactivate it as well as cause agglutination of Microciona cells but not Haliclona cells.…”

Section: Specificity Of Aggregationmentioning

confidence: 99%

The Cell Biology of Sponges

Simpson

1984

691

604

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PrefaceThe writing of this work was stimulated by discussions with colleagues in the fields of biochemistry, developmental biology, and physiology who expressed their, and other's, need for a current, critical review and analysis of sponge cell biology, and secondly by my own conviction that the field of sponge biology in general could benefit from such a synthesis and evaluation of the data. The major problem faced in writing this book was that of balancing the text relative to presenting, on the one hand, a review of the data and, on the other hand, a synthesis of it. A vigorous attempt has been made to be as uniform as possible in reviewing data but this, of course, is not always possible because some papers stand out as key advances in one or more areas. To the extent that it has been feasible to synthesize specific perspectives or to emphasize deficiencies in knowledge, these have been done. A major effort is made to review cellular structure per se since this has not previously been attempted. Some speculations are also to be found and these, hopefully, will not only stimulate new investigations but will also serve as respites from the unavoidable rigidity of data review. Much effort has been expended to avoid errors, although it is probably not possible in an undertaking of this scope to finally arrive at an error-free state. For such of those that do occur by omission or otherwise, I take full and sole responsibility.There are a number of important, recent monographs and symposial volumes which contain much information on sponge cell biology as well as other topics. Outstanding among them is Biologie des Spongiaires (C. Levi and N. Boury-Esnault, Eds., 1979. Editions de CNRS, Paris).

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“…Such cytolytic effects were confirmed by Van de Vyver (1975) and the heteroagglutinating effects have also been noted by others (e.g., Spiegel, 1955). The latter effects were presumed to coincide with the ability of sponge extracts to cause the agglutination of red blood cells (MacLennan and Dodd, 1967).…”

Section: Defense Against Pathogens and Foreign-body Invasionmentioning

confidence: 55%