The rate-limiting enzyme in phosphatidylcholine synthesis is associated with nuclear speckles under stress conditions

Favale, Nicolás Octavio; Fernández-Tomé, María C.; Pescio, Lucila Gisele; Sterín-Speziale, Norma

doi:10.1016/j.bbalip.2010.07.003

Cited by 21 publications

(17 citation statements)

References 46 publications

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“…In our lab, we have demonstrated that under hypertonic conditions, MDCK cells acquire a differentiated phenotype with changes in the cellular and nuclear architecture ( 25,26 ), accompanied by changes in lipid metabolism ( 27,28 ). Considering both that AJ plays a central role in epithelial cell differentiation and our previous observation that AJ is located in an SM-rich membrane domain, we decided to evaluate whether SM synthesis is involved in the acquisition of the differentiated phenotype of MDCK cells induced by external hypertonic stress.…”

Section: Lipid Analysismentioning

confidence: 99%

Sphingomyelin metabolism is involved in the differentiation of MDCK cells induced by environmental hypertonicity

Favale

Santacreu

Pescio

et al. 2015

Journal of Lipid Research

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This article is available online at http://www.jlr.org differentiation, and intracellular traffi cking ( 1 ). It has been demonstrated that cells adjust SL production in response to metabolic needs ( 2 ). SM is biochemically synthesized through the activity of serine-palmitoyl-CoA transferase, 3-ketosphinganine reductase, ceramide (Cer) synthase, dihydroceramide desaturase, and sphingomyelin synthase (SMS). SMS, which uses Cer and phosphatidylcholine as substrates, is the last enzyme in SM biosynthesis ( 3 ). Two isoforms, SMS1 and SMS2, have been cloned in mammals ( 4 ). SMS1 localizes in the Golgi apparatus, whereas SMS2 can be localized in the PM ( 5 ) but also in the Golgi apparatus. Although SM is principally synthesized by SMS1 activity, it has been reported that both SMS1 and SMS2 are required for SM homeostasis and growth in human HeLa cells ( 6 ). SM synthesis is directly related to correct intracellular protein traffi cking ( 7 ). It has been recently shown that the downregulation of SMSs significantly retards the traffi cking of the reporter protein vesicular stomatitis virus G protein tagged with GFP from the trans-Golgi network to the PM. Moreover, the correct endosomal recycling network is directly related to Golgi SM synthesis ( 8 ).SM is the main SL in mammalian cells. Because of the high affi nity of interaction with cholesterol, SM drives the formation of PM rafts or detergent-resistant microdomains (DRMs) ( 9, 10 ), thus providing a framework for PM organization. Early studies have demonstrated

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Section: Lipid Analysismentioning

confidence: 99%

Sphingomyelin metabolism is involved in the differentiation of MDCK cells induced by environmental hypertonicity

Favale

Santacreu

Pescio

et al. 2015

Journal of Lipid Research

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“…Three key enzymes, choline kinase (CK), CTPphosphocholine cytidylyltransferase (CCT), and cytidyltriphosphatecholine transferase (CPT), are involved in the CDP-choline pathway (Tasseva et al, 2004). CK plays a regulatory role in PC synthesis (Tasseva et al, 2004), and CCT is considered to be a rate-limiting enzyme (Favale, Fernández-Tome, Pescio, & Sterin-Speziale, 2010).…”

mentioning

confidence: 99%

Up‐regulation of lipid metabolism and glycine betaine synthesis are associated with choline‐induced salt tolerance in halophytic seashore paspalum

Gao

Zhang

et al. 2019

Plant Cell & Environment

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Choline may affect salt tolerance by regulating lipid and glycine betaine (GB) metabolism. This study was conducted to determine whether alteration of lipid profiles and GB metabolism may contribute to choline regulation and genotypic variations in salt tolerance in a halophytic grass, seashore paspalum (Paspalum vaginatum). Plants of Adalayd and Sea Isle 2000 were subjected to salt stress (200‐mM NaCl) with or without foliar application of choline chloride (1 mM). Genotypic variations in salt tolerance and promotive effects of choline application on salt tolerance were associated with both the up‐regulation of lipid metabolism and GB synthesis. The genotypic variations in salt tolerance associated with lipid metabolism were reflected by the differential accumulation of phosphatidylcholine and phosphatidylethanolamine between Adalayd and Sea Isle 2000. Choline‐induced salt tolerance was associated with of the increase in digalactosyl diacylglycerol (DGDG) content including DGDG (36:4 and 36:6) in both cultivars of seashore paspalum and enhanced synthesis of phosphatidylinositol (34:2, 36:5, and 36:2) and phosphatidic acid (34:2, 34:1, and 36:5), as well as increases in the ratio of digalactosyl diacylglycerol: monogalactosyl diacylglycerol (DGDG:MGDG) in salt‐tolerant Sea Isle 2000. Choline regulation of salt tolerance may be due to the alteration in lipid metabolism in this halophytic grass species.

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“…It is thought that the lamin A/Cassociating foci may act as store for inactive CCTα, whereas the lamin A/C free foci preserve the enzyme for phospholipid synthesis. These observations suggest a role for CCTα, the nucleoplasmic reticulum and lamin A/C in differentiation of MDCK cells under hypertonic conditions [25] and recovery from osmotic stress.…”

Section: Osmotic Stressmentioning

confidence: 91%

The nuclear envelope and its involvement in cellular stress responses

Malhas

Vaux

2011

Biochemical Society Transactions

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The nuclear envelope is not only important for the structural integrity of the nucleus, but also involved in a number of cellular functions. It has been shown to be important for maintaining and controlling chromatin organization, sequestering transcription factors, replication, transcription and signalling. The nuclear envelope is thus important for development and differentiation, and some of its components are essential for cell viability. Among the many functions which are emerging for the nuclear envelope is its involvement in protecting the cell against different types of cellular stress. In the present paper, we review key findings which describe the roles of nuclear envelope components in responses to common types of stress conditions.

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The rate-limiting enzyme in phosphatidylcholine synthesis is associated with nuclear speckles under stress conditions

Cited by 21 publications

References 46 publications

Sphingomyelin metabolism is involved in the differentiation of MDCK cells induced by environmental hypertonicity

Sphingomyelin metabolism is involved in the differentiation of MDCK cells induced by environmental hypertonicity

Up‐regulation of lipid metabolism and glycine betaine synthesis are associated with choline‐induced salt tolerance in halophytic seashore paspalum

The nuclear envelope and its involvement in cellular stress responses

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