The rat prolactin gene: a target for tissue-specific and hormone-dependent transcription factors

Gourdji, D.; Laverrière, Jean-Noël

doi:10.1016/0303-7207(94)90292-5

Cited by 39 publications

(18 citation statements)

References 90 publications

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“…The rat PRL (rPRL) promoter is comprised of a distal enhancer (Ϫ1710 to Ϫ1550), containing an estrogen response element, and a proximal (Ϫ425) promoter region (13,19). This proximal region is sufficient to confer tissue-specific expression and to impart both positive and negative hormonal regulation to the rPRL gene (6,14,23).…”

mentioning

confidence: 99%

“…PRL gene expression is highly restricted to somatomammotroph and lactotroph cells of the anterior pituitary and is subject to regulation by a variety of hormones and second messengers (13,19). The rat PRL (rPRL) promoter is comprised of a distal enhancer (Ϫ1710 to Ϫ1550), containing an estrogen response element, and a proximal (Ϫ425) promoter region (13,19).…”

mentioning

confidence: 99%

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Interaction of Ets-1 and the POU-Homeodomain Protein GHF-1/Pit-1 Reconstitutes Pituitary-Specific Gene Expression

Bradford

Wasylyk

et al. 1997

Molecular and Cellular Biology

107

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2The pituitary-specific, POU-homeodomain factor GHF-1/Pit-1 is necessary, but not sufficient, for cellspecific expression of prolactin (PRL), growth hormone (GH), and thyrotropin. Combinatorial interactions of GHF-1 with other factors are likely to be required; however, such factors and their mechanisms of action remain to be elucidated. Here we identify Ets-1 as a factor that functionally and physically interacts with GHF-1 to fully reconstitute proximal PRL promoter activity. In contrast, Ets-2 has no effect, and the alternatively spliced GHF-2/Pit-1␤ variant fails to synergize with Ets-1. The Ets-1-GHF-1 synergy requires a composite Ets-1-GHF-1 cis element and is dependent on an Ets-1-specific protein domain. Furthermore, the ancestrally related and GHF-1-dependent GH promoter, which lacks this composite element, does not exhibit this response. Finally, Ets-1, but not Ets-2, binds directly to GHF-1 and GHF-2. These data show that a functional interaction of GHF-1 and Ets-1, acting via a composite DNA element, is required to establish lactotroph-specific PRL gene expression, thus providing a molecular mechanism by which GHF-1 can discriminate between the GH and PRL genes. These results underscore the importance of transcription factors that are distinct from, but interact with, homeobox proteins to establish lineage-specific gene expression.

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confidence: 99%

mentioning

confidence: 99%

Interaction of Ets-1 and the POU-Homeodomain Protein GHF-1/Pit-1 Reconstitutes Pituitary-Specific Gene Expression

Bradford

Wasylyk

et al. 1997

Molecular and Cellular Biology

107

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“…Indeed, basal PRL transcription-dependent on GA-binding protein ␣ and ␤1 is responsive to growth factors such as FGF2, FGF4, insulin, insulin growth factor, and EGF, and the action of these factors has been shown to be mediated through Ets factors (33,34). In addition, the action of the Ras-MAP kinase pathway on PRL gene transcription was mapped to Ets factors (30) and shown to depend on both Ets and Pit1 binding sites (32).…”

Section: Discussionmentioning

confidence: 99%

“…The joint action of Etv1 interacting with Tpit for cellspecific transcription is reminiscent of the interaction of other Ets transcription factors with Pit1 for lactotroph-specific transcription of the PRL gene (30,32). Indeed, Ets transcription factors interact with Pit1 for cell-specific transcription of the PRL gene, but they also contribute to basal transcription through binding to a basal transcription element (33). Various members of the Ets family were shown to act on PRL transcription, but direct affinity purification of Ets factors from GH3 cells identified GA-binding protein ␣ and ␤1 as predominant factors (34).…”

Section: Discussionmentioning

confidence: 99%

The Ets Factor Etv1 Interacts with Tpit Protein for Pituitary Pro-opiomelanocortin (POMC) Gene Transcription

Budry

Couture

Balsalobre

et al. 2011

Journal of Biological Chemistry

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“…Multiple hormones, growth factors, and oncogenes act in conjunction with GHF-1 to regulate pituitary-specific expression of the PRL gene. Those factors include ligands for nuclear hormone receptors (11,12), hypophysiotropic peptides that activate the protein kinase A or protein kinase C pathways (13)(14)(15)(16), or ligands of tyrosine kinase growth factor receptors (17)(18)(19)(20). Among the latter, the family of fibroblast growth factors (FGFs) appears to play an important role in pituitary organogenesis (21), in differentiation of lactotropes (22), and recently in the dedifferentiation mechanism for lactotrope tumor pathogenesis (23).…”

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confidence: 99%

Differentiation of Lactotrope Precursor GHFT Cells in Response to Fibroblast Growth Factor-2

López-Fernández¹,

Palacios²,

Castillo³

et al. 2000

Journal of Biological Chemistry

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The mechanisms that control the emergence of different anterior pituitary cells from a common stem cell population are largely unknown. The immortalized GHFT cells derived from targeted expression of SV40 T antigen to mouse pituitary display characteristics of somatolactotropic progenitors in that they express the transcription factor GHF-1 (Pit-1) but not growth hormone (GH) or prolactin (PRL). We searched for factors capable of inducing lactotropic differentiation of GHFT cells. PRL gene expression was not observed in cells subjected to a variety of stimuli, which induce PRL gene expression in mature lactotropes. Only fibroblast growth factor-2 (FGF-2) was able to initiate the transcription, synthesis, and release of PRL in GHFT cells. However, induction of PRL expression was incomplete in FGF-2-treated cells, suggesting that additional factors are necessary to attain high levels of PRL transcription in fully differentiated lactotropes. We also show that the FGF-2 response element is located in the proximal PRL promoter. Stimulation of PRL expression by FGF-2 requires endogenous Ets factors and these factors as well as GHF-1 are expressed at low levels in the committed precursor, suggesting that these low levels are limiting for full PRL expression. Moreover, FGF-2 effect on lactotrope differentiation is mediated, at least partially, by stimulation of the Ras-signaling pathway. Our results suggest that, indeed, GHFT cells represent a valid model for studying lactotropic differentiation and that FGF-2 could play a key role both in initiating lactotrope differentiation and maintaining PRL expression.

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The rat prolactin gene: a target for tissue-specific and hormone-dependent transcription factors

Cited by 39 publications

References 90 publications

Interaction of Ets-1 and the POU-Homeodomain Protein GHF-1/Pit-1 Reconstitutes Pituitary-Specific Gene Expression

Interaction of Ets-1 and the POU-Homeodomain Protein GHF-1/Pit-1 Reconstitutes Pituitary-Specific Gene Expression

The Ets Factor Etv1 Interacts with Tpit Protein for Pituitary Pro-opiomelanocortin (POMC) Gene Transcription

Differentiation of Lactotrope Precursor GHFT Cells in Response to Fibroblast Growth Factor-2

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