The rat neutrophil low-affinity Fc receptor for IgG: molecular cloning and functional characterization

Yasuhiro, Isashi; Tamakoshi, Masatada; Nagai, Y.; Sudo, Tetsuo; Murakami, Masaaki; Uede, Toshimitsu

doi:10.1016/0165-2478(95)00037-6

Cited by 2 publications

(2 citation statements)

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“…Ab probes that were used would then bind to the Fc region of the Ab connected to the cells FcR, thereby giving a false positive signal and counted as an Ab-expressing B cell. These occurrences, though, were conceivably extremely minimal, because most nonspecific cells in rats have only been shown to express one type of FcR on the surface (37,38), or their expression was not consistent with the dual-Ab tested for (39)(40)(41). Neutrophils have FcR for IgG and IgA (Isashi et al 1995), but have not been found to bind IgE.…”

Section: Discussionmentioning

confidence: 99%

“…These occurrences, though, were conceivably extremely minimal, because most nonspecific cells in rats have only been shown to express one type of FcR on the surface (37,38), or their expression was not consistent with the dual-Ab tested for (39)(40)(41). Neutrophils have FcR for IgG and IgA (Isashi et al 1995), but have not been found to bind IgE. Eosinophils and basophils express FcR only for IgE (37), and Macrophages only subtypes of IgG (38).…”

Section: Discussionmentioning

confidence: 99%

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Rapidity and multiplicity of synthesis and expression of immunoglobulin isotypes by B lymphocytes in the small intestine

Wagner

Montalvo²,

Hauschild³

et al. 2004

Front Biosci

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An immunofluorescence double labeling assay was used to examine the kinetics of intestinal B lymphocytes with concurrent expression of multiple antibody isotypes in the mucosal tissues of rats infected with Trichinella spiralis (TS) muscle larvae for 1 to 15 days. As compared to the uninfected controls (day 0), the non-Peyer's patch tissues of the small intestine contained a significantly increased number of dual antibody-expressing B cells as early as 3 days after infection with a maximum proliferation of these B cells on days 7 and 10. These results indicate the rapidity of B cell response in the small intestine. Similar results were observed in the germinal centers of the Peyer's patches. The non-germinal centers of the Peyer's patch tissues showed delayed kinetics in B cell activation which occurred 10 days after infection. Quantification of the total number of B cells in these tissues was also carried out by staining the CD45RA marker on B cells with the OX33 monoclonal antibody. When comparing the total numbers of B cells with the numbers of B cells expressing dual isotypes of antibodies, our results showed the numbers of dual-expressing B cells (IgA:IgE, IgM:IgE, IgG1:IgE, IgG2a:IgE, IgG2b:IgE, and IgG2c:IgE), when combined, were over 7 times that of the total number of OX33-labeled B cells on day 7 in the small intestine. The dual-expressing B cells in the Peyer's patch-germinal center were more than 5 times that of the OX33-labeled B cells on day 15. These results therefore suggest that the dual-expressing B cells most likely synthesized and expressed more than two isotypes of antibodies during the peak days of the humoral response. Such phenomenon was not observed in non-germinal centers of the Peyer's patch tissues.

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