The random amplified polymorphic DNA (RAPD) assay and related techniques applied to genotoxicity and carcinogenesis studies: A critical review

Atienzar, Franck; Jha, Awadhesh N.

doi:10.1016/j.mrrev.2006.06.001

Cited by 296 publications

(236 citation statements)

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“…The disappearance of normal bands may be related to the DNA damage (e.g. single-strand breaks, double-strand breaks, modified or oxidized bases, bulky adduct), point mutations and/or complex chromosomal rearrangements induced by genotoxic chemicals (Atienzar and Jha 2006). The highest number of disappeared bands that was observed with zinc and nickel at both concentrations suggests that these metals maybe cause one of these changes to DNA of the treated plants that consequently resulted in the disappearance of DNA bands.…”

Section: Resultsmentioning

confidence: 99%

“…Advantages of measuring effects of genotoxic chemicals directly on DNA are mainly related to the sensitivity and short response time. DNA based techniques like Random Amplified Polymorphic DNA (RAPD) is used to evaluate the variation at the DNA level and can clearly be shown when comparing DNA fingerprints from untreated and treated individuals to genotoxic agents (Savva 1998;Atienzar and Jha 2006;Enan 2006;Kekec et al 2010).…”

Section: Introductionmentioning

confidence: 99%

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Mineral nutrient imbalance, total antioxidants level and DNA damage in common bean (Phaseolus vulgaris L.) exposed to heavy metals

Ackova

Panovska

Ruskovska

et al. 2013

Physiol Mol Biol Plants

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The present study aimed to analyze the biological effects induced by bioaccumulation of metals in common bean (Phaseolus vulgaris L.). Effects of mineral nutrient imbalance, total antioxidants level and DNA damage induced by accumulation of heavy metals, were investigated in bean seedlings treated with two selected metal concentrations for 7 days. Metal content is analyzed by inductively coupled plasma -atomic emission spectrometer (ICP-AES), for total antioxidants level assessment the Ferric-Reducing Antioxidant Power (FRAP) assay is used and Random Amplified Polymorphic DNA (RAPD) method was applied for investigation of DNA damages. The increasing metal concentration in the treatment medium changed synchronously metal content in samples, and decreased total antioxidant activity in all samples with exception only for samples treated with Ni and Cd. The obtained "DNA fingerprints" demonstrated that the increasing metal concentrations induced changes in RAPD profiles (disappearance and/or appearance of bands in comparison with untreated control samples). The highest number of missing bands was observed in samples treated with zinc (total 4 bands) and nickel (total 4 bands) at both concentrations. These results suggested that mineral nutrient imbalance is involved in changes of antioxidant levels and DNA damages of the seedlings, which may help to understand the mechanism of metal toxicity in plants.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Mineral nutrient imbalance, total antioxidants level and DNA damage in common bean (Phaseolus vulgaris L.) exposed to heavy metals

Ackova

Panovska

Ruskovska

et al. 2013

Physiol Mol Biol Plants

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“…For detecting genotoxicity, the RAPD-PCR (Randomly Amplified Polymorphic DNA) technique was employed and photosynthetic pigment contents were evaluated in order to make assessments for the effects of Cd toxicity. Using RAPD-PCR in assessing of genotoxic effects of heavy metals is commonly used technique, which enables to detect the DNA band features such as band intensity and gain or loss in relation to toxicant exposures (Atienzar & Jha, 2006;Swaileh et al, 2008;Kekec et al, 2010). Therefore, this study results will pave the way for understanding of alterations in genetic material in relation to heavy metal exposure such as Cd as well as in changes of metabolic processes relatedly.…”

Section: Introductionmentioning

confidence: 90%

Detection of physiological and genotoxic damages reflecting toxicity in Kalanchoe clones

Demir¹,

Tombuloğlu²,

Sakcali³

et al. 2016

Issue 1

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In order to make assessments in understanding of physiological and genotoxic effects of imposing cadmium (Cd) on photosynthetic pigment contents along with the changes occurring in genetic material of Kalanchoe plants were used in relation to various Cd-treatments. Young plantlets were originated from a single host plant as clones, and developed in vitro. Developed clones were grown in standard pots with daily watering of Hoagland solution (20 ml) containing different concentrations of cadmium chloride for two months. Cd concentrations of the collected samples were measured by employing ICP-OES and RAPD-PCR technique was applied for detecting the genotoxic effects of Cd. After two month of experimental period, the comparisons between unexposed and exposed Kalanchoe clone groups revealed reductions in photosynthetic pigment contents, especially at the highest level of Cd exposure and a genomic instability when application of Cd concentration increases. RAPD-PCR analyses demonstrated the distinguishable banding pattern in number and band intensities between Cd-treated and control groups. In addition, progressive Cd accumulations in leaves, stems and roots of plant samples were observed when the application of exposure level increased.

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“…The RAPD method has been initially used to detect polymorphism in genetic mapping, taxonomy and phylogenetic studies and later in genotoxicity and carcinogenesis studies (Atienzar and Jha 2006;Fouz et al, 2007). In addition, the fact that the RAPD assay allows the visualization of a wide range of PCR products may explain why this assay is a preferred choice.…”

Section: Introductionmentioning

confidence: 99%

Genetic relationship between five fish species of genus scarus using rapd assay

Almaaty

El-Regal

Marie

et al. 2015

Egypt. J. of Aquatic Biolo. and Fish.

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Scaridae fish species including Scarus psittacus, S. frenatus, S. genazonatus, S. fuscopurpureus and S. niger, were collected from Red Sea in Egypt. The DNA fingerprints of these species were identified using RAPD-PCR technique with eight decamer primers. A total of 93 bands were scored in all species using the selected primers, with the size range varying from 190 to 2500 bp. On average, each primer amplified 11.63 bands per species. Each species had both monomorphic and polymorphic DNA bands. The polymorphic bands represent 34.41% in all fish species. The highest similarity index (70%) was found between Scarus psittacus and S. frenatus, while the lowest one (51%) was between Scarus psittacus and S. niger, S. genazonatus and S. fuscopurpureus and S. genazonatus and S. niger. Therefore the phylogenetic relationships among the five studied scaridae species were clearly resolved in dendrogram, they were clustered into four clusters groups: the first one comprised, S. fuscopurpureus and S. niger, the second included S. psittacus, S. frenatus and S. genazonatus, the third included S. genazonatus and the fourth branch contained S. psittacus and S. frenatus. In conclusion, the obtained results exhibited that the five Scarus species are distinctive ones. In addation, the RAPD patterns are suitable tools to differentiate between fish species.

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The random amplified polymorphic DNA (RAPD) assay and related techniques applied to genotoxicity and carcinogenesis studies: A critical review

Cited by 296 publications

References 161 publications

Mineral nutrient imbalance, total antioxidants level and DNA damage in common bean (Phaseolus vulgaris L.) exposed to heavy metals

Mineral nutrient imbalance, total antioxidants level and DNA damage in common bean (Phaseolus vulgaris L.) exposed to heavy metals

Detection of physiological and genotoxic damages reflecting toxicity in Kalanchoe clones

Genetic relationship between five fish species of genus scarus using rapd assay

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