The radiation resistance of the bioburden from medical devices

Takehisa, Masaaki; Shintani, Hideharu; Sekiguchi, Masayuki; Koshikawa, Tomihiko; Oonishi, T.; Tsuge, M.; Sou, K.; Yamase, Yutaka; Kinoshita, Shin; Tsukamoto, Hidekazu; Endô, Takayuki; Yashima, K.; Nagai, Maria L.E.; Ishigaki, K.; Sato, Yushi; Whitby, J. L.

doi:10.1016/s0969-806x(98)00022-x

Cited by 11 publications

(8 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…At the other extreme, when the density of double-strand breaks reaches ~100 per genome equivalent, no repair or survival becomes possible. These numbers are most likely similar to other bacterial and eukaryotic cells alike [33, 80], as the massive experience in radiation sterilization testifies [81, 82]. The only exception is Deinococcus radiodurans and its relatives, capable of assembling chromosome with no loss of viability after 100 double-strand breaks per genome equivalent [83-85].…”

Section: Discussionmentioning

confidence: 97%

Prompt repair of hydrogen peroxide-induced DNA lesions prevents catastrophic chromosomal fragmentation

Mahaseth

Kuzminov

2016

DNA Repair

View full text Add to dashboard Cite

Iron-dependent oxidative DNA damage in vivo by hydrogen peroxide (H2O2, HP) induces copious single-strand(ss)-breaks and base modifications. HP also causes infrequent double-strand DNA breaks, whose relationship to the cell killing is unclear. Since hydrogen peroxide only fragments chromosomes in growing cells, these double-strand breaks were thought to represent replication forks collapsed at direct or excision ss-breaks and to be fully reparable. We have recently reported that hydrogen peroxide kills Escherichia coli by inducing catastrophic chromosome fragmentation, while cyanide (CN) potentiates both the killing and fragmentation. Remarkably, the extreme density of CN+HP-induced chromosomal double-strand breaks makes involvement of replication forks unlikely. Here we show that this massive fragmentation is further amplified by inactivation of ss-break repair or base-excision repair, suggesting that unrepaired primary DNA lesions are directly converted into double-strand breaks. Indeed, blocking DNA replication lowers CN+HP-induced fragmentation only ~2-fold, without affecting the survival. Once cyanide is removed, recombinational repair in E. coli can mend several double-strand breaks, but cannot mend ~100 breaks spread over the entire chromosome. Therefore, double-strand breaks induced by oxidative damage happen at the sites of unrepaired primary one-strand DNA lesions, are independent of replication and are highly lethal, supporting the model of clustered ss-breaks at the sites of stable DNA-iron complexes.

show abstract

Section: Discussionmentioning

confidence: 97%

Prompt repair of hydrogen peroxide-induced DNA lesions prevents catastrophic chromosomal fragmentation

Mahaseth

Kuzminov

2016

DNA Repair

View full text Add to dashboard Cite

show abstract

“…As células que não têm a divisão celular com duplicação do código genético podem passar por várias quebras sem afetar suas funções. 24 Caso haja o rearranjo dos fragmentos resultantes das quebras de DNA, pode ocorrer o surgimento de cromossomos modificados em relação à estrutura original. Células contendo cromossomos modificados podem se duplicar dando origem a uma população de células anormais.…”

Section: Efeitos Das Radiações Na Molécula De Dnaunclassified

The Interaction of Gamma Radiation with the Matter in the Process of Sterilization

Silva¹,

Silva²,

Aquino³

2014

Revista Virtual De Química

View full text Add to dashboard Cite

Abstract:The sterilization by gamma radiation is used as an international standard and its use has grown substantially in national processes. In this review, it is treated the interaction of ionizing radiation with matter and its effects, in addition to various applications of gamma irradiation as an effective tool for sterilization and decontamination of polymeric materials or reducing microbial load in food and packaging fields. This is a method that consists of exposing the products to the action of short electromagnetic waves generated from sources of Cobalt 60 and Cesium 137 in a specially prepared environment for this procedure. As electromagnetic waves have great penetrating power, organisms can be reached wherever they are, both in sealed packages as products packaged in the various forms, which ensures the overall effectiveness of the process.Keywords: Sterilization; Gamma radiation; Ionization; Interaction; Biological effect. ResumoA esterilização via radiação gama é utilizada como padrão internacional e o seu uso vem crescendo substancialmente nos processos nacionais. Nesta revisão será tratado o processo de interação da radiação ionizante com a matéria e seus efeitos, além das diversas aplicações de irradiação por raios gama como ferramenta eficiente para esterilização de materiais poliméricos e descontaminação ou redução de carga microbiana nos segmentos de alimentação e embalagens. Este é um método que consiste na exposição dos produtos à ação de ondas eletromagnéticas curtas, geradas a partir de fontes de Cobalto 60 e Césio 137 em um ambiente especialmente preparado para esse procedimento. Como as ondas eletromagnéticas possuem grande poder de penetração, os organismos podem ser alcançados onde quer que estejam, tanto em embalagens lacradas como em produtos acondicionados das mais variadas maneiras, o que garante a total eficácia do processo.

show abstract

“…Several types of microorganism, mainly bacteria and, less frequently, moulds and yeasts, have been found on many medical devices and pharmaceuticals (Takehisa et al, 1998). Complete eradication of these microorganisms (sterilization) is essential to the safety of medical devices and pharmaceutical products.…”

Section: Effects Of Gamma Rays On Living Organismsmentioning

confidence: 99%

“…sterility assurance level (SAL), required for that product. Because of this reason, the optimum sterilization dose is 25 kGy at the above level of bioburden (Takehisa et al, 1998). …”

Section: Sterilization Dosementioning

confidence: 99%

Sterilization by Gamma Irradiation

Aquino¹

2012

Gamma Radiation

View full text Add to dashboard Cite

The radiation resistance of the bioburden from medical devices

Cited by 11 publications

References 3 publications

Prompt repair of hydrogen peroxide-induced DNA lesions prevents catastrophic chromosomal fragmentation

Prompt repair of hydrogen peroxide-induced DNA lesions prevents catastrophic chromosomal fragmentation

The Interaction of Gamma Radiation with the Matter in the Process of Sterilization

Sterilization by Gamma Irradiation

Contact Info

Product

Resources

About