The quantification of ellagic acid in the crude extract of Phyllanthus amarus Schum. & Thonn. (Euphorbiaceae)

Abstract

Based on the obtained results, it can be concluded that the newly developed method is suitable for its purpose, namely the determination of ellagic acid in the crude extract of P. amarus.

Antimalarial activities of medicinal plants traditionally used in the villages of Dharmapuri regions of South India

“…Earlier, we have reported promising antiplasmodial activity in the leaf ethyl acetate and methanol extract of Phyllanthus emblica (IC 50 = 7.25 and 3.125 g/mL, respectively) (Bagavan et al, 2011b). Our finding of the presence of antimalarial activity in Phyllanthus amarus is supported by previous reports which indicated ellagic acid (formed by hydrolysis of tannins) as the active constituent of an ethanolic extract of the same plant (Dhooghe et al, 2011). Xiang et al (2011) isolated a polyphenolic compound 1,2,4,6-tetra-O-galloyl-␤-d-glucose (1246TGG) from Phyllanthus emblica having in vitro antiviral activities against herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2).…”

Phyllanthus amarus: Ethnomedicinal uses, phytochemistry and pharmacology: A review

“…The overall mean content of ellagic acid was 2.06%. A recovery experiment was performed and it showed an accuracy of 100.4% (Dhooghe et al, 2011). A simple, specific and precise RP-HPLC method has been developed and validated for the estimation of phyllanthin, present in P. amarus.…”

Development of a UHPLC–MS/MS method for the quantitation of bioactive compounds in Phyllanthus species and its herbal formulations

“…Standards of quinic acid (1), gallic acid (2), protocatechuic acid (3), catechin (4), chlorogenic acid (5), epicatechin (6), chebulagic acid (7), quercetin-3,4'-di-O-glucoside (8), chebulinic acid (9), ellagic acid (10), rutin (11), naringin (12), kaempferol-3-O-rutinoside (13), eriodictyol (14), scutellarein (15), quercetin (16), luteolin (17), kaempferol (18), amentoferine (19), betulinic acid (22), and oleanolic acid (23) were purchased from Sigma-Aldrich (St. Louis, MO, USA; Supporting Information Fig. S1).…”

“…The quantitative analysis of bioactive lignans has been carried out in Phyllanthus species by traditional methods such as high-performance thin-layer chromatography (HPTLC) and HPLC [13][14][15][16][17][18][19][20][21][22][23][24][25]. Several researchers have reported fingerprinting, identification and characterization of bioactive compounds in crude extracts of Phyllanthus species using LC-MS [24][25][26][27][28][29][30][31][32][33][34].…”