The purification of mouse monoclonal antibodies from ascitic fluid

Comparison of six methods for the extraction of lipids from serum in terms of effectiveness and protein preservation

“…This was only attained when the silica concentration was raised ten times, in two consecutive absorption steps lasting 2 and 16 h. It is possible that the lipid amounts in the samples used by Neoh et al [13] were smaller than those usually found in human serum.…”

“…The extraction method using silica, described by Neoh et al [13], which involves a 30-min absorption step, did not efficiently remove lipids from human serum. This was only attained when the silica concentration was raised ten times, in two consecutive absorption steps lasting 2 and 16 h. It is possible that the lipid amounts in the samples used by Neoh et al [13] were smaller than those usually found in human serum.…”

“…This was carried out according to a modification of Neoh et al's methodology [13]. To 1 ml of serum, diluted 1:2 in 0.05 M borate buffer, pH 8.3, 360 mg of silica were added (Sigma, St. Louis, USA).…”

The prolonged and enhanced immune response in the non-obese diabetic mouse is dependent on genes in the Idd1/24, Idd12 and Idd18 regions

“…[25], which was purified from hybridoma supernatants by PEG6000 precipitation [26] and dialyzed against PBS. Mice were immunized intravenously with 100 mg BA.N 1:1.8 in the tail vein and blood was collected at different time points as indicated.…”

Production and characterisation of monoclonal anti-idiotype antibody to Vibrio anguillarum

“…Mouse anti-V. anguillarum mab 4A6 was prepared and purified from ascitic fluid by 5% (w/v) polyethylene glycol 6000 (PEG) precipitation as described by Neho et al [14]. mab 1 4A6 is of the IgG 1 isotype.…”