Comparison of six methods for the extraction of lipids from serum in terms of effectiveness and protein preservation

Ferraz, T.P.L.; Fiuza, Manuela; Santos, M.L.A dos; Carvalho, Lidiane dos Santos; Soares, Neci Matos

doi:10.1016/j.jbbm.2003.10.008

Cited by 69 publications

(38 citation statements)

References 10 publications

(11 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Serum TG (Stanbio, Boerne, TX) and lactate (Biovision Inc., Milpitas, CA) levels were measured using colorimetric kits based on the protocol recommended by the manufacturer. For the hepatic TG measurement, we used a modified protocol that was previously described by Ferraz 24 . Briefly, snap-frozen liver samples (30–50 mg) were homogenized using a motorized chuck and Teflon pestle in 1% Triton-x-100 in PBS at a concentration of 1 mg/dL.…”

Section: Methodsmentioning

confidence: 99%

p32 heterozygosity protects against age- and diet-induced obesity by increasing energy expenditure

Liu

Leslie

Jin

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Obesity is increasing in prevalence and has become a global public health problem. The main cause of obesity is a perturbation in energy homeostasis, whereby energy intake exceeds energy expenditure. Although mitochondrial dysfunction has been linked to the deregulation of energy homeostasis, the precise mechanism is poorly understood. Here, we identify mitochondrial p32 (also known as C1QBP) as an important regulator of lipid homeostasis that regulates both aerobic and anaerobic energy metabolism. We show that while whole-body deletion of the p32 results in an embryonic lethal phenotype, mice heterozygous for p32 are resistant to age- and high-fat diet-induced ailments, including obesity, hyperglycemia, and hepatosteatosis. Notably, p32 +/− mice are apparently healthy, demonstrate an increased lean-to-fat ratio, and show dramatically improved insulin sensitivity despite prolonged high-fat diet feeding. The p32 +/− mice show increased oxygen consumption and heat production, indicating that they expend more energy. Our analysis revealed that haploinsufficiency for p32 impairs glucose oxidation, which results in a compensatory increase in fatty acid oxidation and glycolysis. These metabolic alterations increase both aerobic and anaerobic energy expenditure. Collectively, our data show that p32 plays a critical role in energy homeostasis and represents a potential novel target for the development of anti-obesity drugs.

show abstract

Section: Methodsmentioning

confidence: 99%

p32 heterozygosity protects against age- and diet-induced obesity by increasing energy expenditure

Liu

Leslie

Jin

et al. 2017

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Targeted compounds such as triglyceride can be separated by mixing the solution to a temperature near to boiling of solvent which will evaporate solvent but targeted compounds will not [9]. The role of methanol is to extract triglyceride, phospholipids, cholesterol, etc [10]. The extracted lipid was analysed in gas chromatograph mass spectrophotometer (GC-MS) to determine the presence of free fatty acids.…”

Section: Lipid Analysismentioning

confidence: 99%

Utilization of kitchen food waste for biodiesel production

Barik

Paul

Priyadarshi

2018

IOP Conf. Ser.: Earth Environ. Sci.

View full text Add to dashboard Cite

Abstract. Food waste has led to handling and disposal problems affecting human health and polluting environment. Land disposal is one of the ways of disposing food waste but it produces harmful leachate when rain water falls on it. Reuse of food waste for biodiesel production is one of the key steps to reduce food waste problem and also meet the energy demand. Food waste in the present study was collected from the kitchen of a hostel of an educational institute located in India. This waste was dried by various drying methods to dewater. Lesser is the moisture content, more will be the lipid extraction and hence increased biodiesel production. Lipids were extracted and fatty acids (caproic acid (6:1), lauric acid (12:0), mystric acid (14:0), palmitic acid (16:0), stearic acid (17:0) and oleic acid (18:0) were identified in gas chromatography-mass spectrometry (GC-MS). These fatty acids identified in lipid indicate the potential of food waste for biodiesel production. Transesterification of lipid was performed to produce biodiesel and concentration of fatty acid methyl ester was determined using gas chromatograph flame ionisation detector (GC-FID). Further, biodiesel properties were compared with various standards.

show abstract

“…The redissolved samples were then defatted with 100 mL chloroform/methanol (2:1 v/v) and 5 mL 1% NaCl following the Bligh and Dyer method (Bligh and Dyer 1959). Furthermore, as it was cited in a comparison report (Ferraz et al 2004), the combination of 2:1 chloroform/methanol (v/v) was effective for removal of lipids and proteins. Other reports (Chen and Chou 1998;Kurono et al 2001;Jang and Yotsu-Yamashita 2006;Jang, Lee, and Yotsu-Yamashita 2010) used chloroform as the lipid removal solvent in puffer fish.…”

Section: Sample Preparationmentioning

confidence: 99%

Evaluation of Helically Coiled and Knitted Open Tubular Reactors for the Efficient Post-Column Determination of Tetrodotoxin by High-Performance Liquid Chromatography

et al. 2016

View full text Add to dashboard Cite

A post-column fluorescence reaction system for the high-performance liquid chromatographic (HPLC) determination of tetrodotoxin in the silver-cheeked toadfish Lagocephalus sceleratus is discussed theoretically and investigated experimentally. Ion-pair chromatography with volatile ammonium perfluoroheptanoate was used for the separation of tetrodotoxin and 4, 9-anhydrotetrodotoxin. The post-column reaction was based on tetrodotoxin conversion to a quinazoline fluorescent compound in strong alkaline conditions. All post-column parameters were optimized that affected the sensitivity, dispersion, and stability. Helically coiled and knitted open tubular reactors composed of polyetheretherketone were constructed and characterized in detail. The performance of these reactors was evaluated on the bases of sensitivity and dispersion. Their optimal design is reported. The knitted reactors were more efficient than the relevant helically coiled reactors when higher reaction times are required. A 1,500 μL polyetheretherketone knitted coil with 0.010″ internal diameter was optimum exhibiting higher pressure tolerances than Teflon coils. The HPLC post-column reaction method was evaluated in Downloaded by [University of Arizona] at 09:01 08 June 20163 terms of linearity, sensitivity, accuracy, precision, and ruggedness. The linear dynamic ranges for tetrodotoxin and 4, 9-anhydrotetrodotoxin were 40 to 3,000 ppb and 80 to 3,000 ppb, respectively. The limits of detection and quantification were 12 and 41 ppb for tetrodotoxin and 26 and 85 ppb for 4, 9-anhydrotetrodotoxin. The accuracy was evaluated by recovery measurements and the values for tetrodotoxin were between 90.7% and 93.6%. The use of a volatile perfuorocarboxylic acid as an ion-pair reagent allowed confirmation of by highperformance liquid chromatography -tandem mass spectrometry using identical mobile phase conditions.

show abstract

Comparison of six methods for the extraction of lipids from serum in terms of effectiveness and protein preservation

Cited by 69 publications

References 10 publications

p32 heterozygosity protects against age- and diet-induced obesity by increasing energy expenditure

p32 heterozygosity protects against age- and diet-induced obesity by increasing energy expenditure

Utilization of kitchen food waste for biodiesel production

Evaluation of Helically Coiled and Knitted Open Tubular Reactors for the Efficient Post-Column Determination of Tetrodotoxin by High-Performance Liquid Chromatography

Contact Info

Product

Resources

About