The Purification and Characterization of the Dextranase of<i>Chaetomium gracile</i>

Hattori, Atsushi; Ishibashi, Keijiro; Minato, Sadamasa

doi:10.1080/00021369.1981.10864911

Cited by 15 publications

(7 citation statements)

References 4 publications

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“…The specificity of the endodextranase of L. starkeyi seems very similar to those of Penicillium [19], Aspergillus [21], Chuetomium [18] and the Lipomyces enzyme reported by Webb and Spencer-Martins [8]. Following the progression of reaction products with time, it is apparent that the minimum glucan chain length that will be hydrolyzed by the enzyme is four, although isomaltoterose was not hydrolyzed as quickly as was isomaltopentose.…”

Section: Reaction End Productsmentioning

confidence: 74%

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The purification and characterization of a dextranase from Lipomyces starkeyi

Koenig¹,

Day²

1989

European Journal of Biochemistry

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Dextranase produced by Lipomyces starkeyi was purified 43-fold, by carboxymethyl-Sepharose chromatography followed by agarose gel-filtration chromatography. The purified enzyme showed four bands by SDS/ polyacrylamide gel electrophoresis with estimated mass 74 kDa, 71 kDa, 68 kDa and 65 kDa. This preparation exhibited multiple isoelectric points between 5.6 and 6.1. All the isoelectric forms were active and catalytically similar. The dextranase contained a carbohydrate moiety (8%). The physical properties of the enzyme were pH and temperature optima of 5.0 and 55"C, respectively. This dextranase was stable between pH 2.5 and 7.0 at temperatures below 40 "C. Lipomyces dextranase was a typical endodextranase with the final product of dextran hydrolysis being isomalto-oligosaccharides from glucose to isomaltotetrose.

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Section: Reaction End Productsmentioning

confidence: 74%

“…The Penicillium enzyme also has a requirement of a sulfhydryl group for activity [19]. Mercuric chloride had the same effect on the dextranase from Chuetomium [18].…”

Section: Reaction End Productsmentioning

confidence: 93%

The purification and characterization of a dextranase from Lipomyces starkeyi

Koenig¹,

Day²

1989

European Journal of Biochemistry

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“…Digestion of six dextrans with an endodextranase from Chaetomium gracile 8 ) gave d.h. (degree of hydrolysis) values ranging from 22.3 to 44.0% (Table II). Three dextrans from B-1307, B-1355, and B-1416 showed somewhat higher.…”

Section: Structure Of the Synthesized Dextransmentioning

confidence: 99%

“…In this paper, we describe a comparative study on the multiple form of these Leuconostoc dextransucrases. 75,000 ± 25,000) and endodextranase preparation from Chaetomium gracile 8 ) were kindly provided by Meito Sangyo Co., and Sankyo Co., Japan, respectively. Dextranase from Flavobacterium sp.…”

mentioning

confidence: 99%

Comparison of the Multiplicity of Dextransucrases from Six Strains ofLeuconostoc mesenteroides

Yokoyama

Kobayashi

Matsuda

1985

Agricultural and Biological Chemistry

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“…Chaetomium gracile SANK 18667 (a gift from Sankyo Co., Ltd, Tokyo) was cultured as in [4]. Candida albicans serotype A, strain K [5] (provided by Dr Aoki), was cultured aerobically in YPG medium.…”

Section: Strains Plasmids and Mediamentioning

confidence: 99%

A simple method for extraction of DNA from fungi and yeasts with anhydrous hydrogen fluoride

et al. 1987

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A novel method is described for the extraction of DNAs from fungi and yeasts. Anhydrous hydrogen fluoride (HF) selectively cleaves their cell walls under mild conditions (for 5 min at 0°C), enabling the effective extraction of DNAs from organisms with a cell wall. A possible mechanism for this method concerning the selective cleavage of O-glycosidic linkages in cell walls has been described previously [(1977) Anal. Biochem. 82, 289-309]. The extracted DNA is intact: in fact, the yeast DNA is directly applicable for restriction analysis and transformation of Escherichia coli.

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The Purification and Characterization of the Dextranase ofChaetomium gracile

Cited by 15 publications

References 4 publications

The purification and characterization of a dextranase from Lipomyces starkeyi

The purification and characterization of a dextranase from Lipomyces starkeyi

Comparison of the Multiplicity of Dextransucrases from Six Strains ofLeuconostoc mesenteroides

A simple method for extraction of DNA from fungi and yeasts with anhydrous hydrogen fluoride

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