The PTB single ion microbeam for irradiation of living cells

Greif, K.-D.; Brede, H.J.; Frankenberg, D.; Giesen, U.

doi:10.1016/j.nimb.2003.11.082

Cited by 66 publications

(33 citation statements)

References 10 publications

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“…Primary HUVEC cells cultures, predominantly in G0/G1 phase of cell cycle, were exposed to ion microbeam irradiation using the facility at the PhysikalischTechnische Bundesanstalt (PTB) [3]. The microbeam cell irradiation system enables hitting individual cell nuclei with a pre-determined number of particles in a given pattern of irradiation.…”

Section: Cell Irradiation Using a Microbeam And Dna Damage Detectionmentioning

confidence: 99%

“…For each nucleosome, the histone proteins were represented by a cylinder of 2.4 nm radius and were surrounded by a DNA double helix composed of 200 pairs of nucleotides. Each of these nucleotides was composed of 3 adjacent spherical volumes of 0.091 nm 3 , 0.060 nm 3 and 0.093 nm 3 representing the sugar, phosphate and base molecules, respectively. A liquid water shell of 12 molecules per nucleotide around the DNA structure was also taken into account as a part of the target for the direct effects calculations.…”

Section: Geometrical Model For Dna Targetmentioning

confidence: 99%

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Geant4-DNA simulation of DNA damage caused by direct and indirect radiation effects and comparison with biological data.

et al. 2017

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Abstract. In this work we present results obtained in the frame of the BioQuaRT project. The objective of the study was the correlation between the number of radiation-induced double strand breaks (DSB) of the DNA molecule and the probability of detecting nuclear foci after targeted microbeam irradiation of cells with protons and alpha particles of different LET. The former were obtained by simulation with new methods integrated into Geant4-DNA that permit calculating the number of DSB in a DNA target model induced by direct and indirect radiation effects. A particular focus was laid in this work on evaluating the influence of different criteria applied to the simulated results for predicting the formation of a direct SSB. Indeed, these criteria have an important impact on the predicted number of DSB per particle track and its dependence with LET. Among the criteria tested in this work, the case that a direct radiation interaction leads to a strand break if the cumulative energy deposited in the backbone part of one nucleotide exceeds a threshold of 17.5 eV leads to the best agreement with the relative LET dependence of number of radiation induced foci. Further calculations and experimental data are nevertheless needed in order to fix the simulation parameters and to help interpreting the biological experimental data observed by immunofluorescence in terms of the DSB complexity.

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Section: Cell Irradiation Using a Microbeam And Dna Damage Detectionmentioning

confidence: 99%

Section: Geometrical Model For Dna Targetmentioning

confidence: 99%

Geant4-DNA simulation of DNA damage caused by direct and indirect radiation effects and comparison with biological data.

et al. 2017

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“…Setups with pre-cell detectors, using thin plastic scintillation detectors or secondary electrons detected in e.g. a channeltron, are described at CENBG [14], GCI [15], PTB [16]. An exit window of 100 nm Si 3 N 4 or thin boron doped diamond films [17] can serve both as window and scintillator (if covered with thin CsI generates secondary electrons).…”

Section: Pre-cell Detectionmentioning

confidence: 99%

At the Tip of an MeV Beam: Provoking Cells and Performing Tomographic Imaging

et al. 2009

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Biological applications of ion beams have recently become a new important research field using single ion hit facilities to study individual living cells and their response to the hit of a counted number of ions. One motivation is the search for a better understanding of the fundamental processes taking place in cells and organs as a result of irradiation. Another comes from the increasing interest in using high energy protons and heavy ions as a modality for radiotherapy of deep seated tumours. In the view of treatment efficiency, study of cell culture behaviour under controlled radiation experiments, and in different chemical environments at single ion hit facilities, is a first step towards a better understanding of the processes. Tomographic techniques are applicable to situations where you need information of the inside of an object but do not want to section it into thin slices or cannot do it. Using focused MeV ion beams for tomography restricts the sample size to the order of 10-100 µm, depending of the initial energy. On the other hand, the ability to focus at a sub-micrometer level makes ion beams well suited for analyses of small sized objects as cells, spores, etc. The scanning transmission ion microscopy mode of tomography gives the mass density and corresponding morphological structure of holes and pores. It can then be used to correct the results from the other mode, particle induced X-ray emission tomography. Here is discussed a porosity analysis of bentonite clay that is planned to form an important buffer zone around canisters filled with spent nuclear reactor fuel waste deposited 500 m underground in Sweden. Development of irradiation facilitiesDuring the recent years there has been a European move towards developing high resolution ion beam facilities to be used in the study of cellular responses to single ion hits [1,2]. Those systems utilise the counted, localised and focused MeV ions to interact with monolayer biological experimental systems to induce localised, quantified damage with the purpose to study the biological effects originating from the interaction. It has since more than a decade been a research field in the borderland between physics and biology [3,4] and today a considerable number of single ion hit facilities have been developed worldwide (see e.g. [5,6]). A single ion hit facility (SIHF) is a modified nuclear microprobe specially adopted for the task of shooting single, positioned ions [7].Many experiments focus on the effects of low dose irradiation: bystander effect, genomic instability and adaptive responses [8]. There are several phenomena that * corresponding author; e-mail: Jan.Pallon@nuclear.lu.se are not well understood, e.g. the limits for double-strain breaks, cell-to-cell communication and interactions that indicate both increased sensitivity versus radiation as well as increased protection against radiation (adaptative response). This type of experiments can hardly be done without the use of well controlled ion exposures (local energy transfer to the cell, num...

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“…The Physikalisch-Technische Bundesanstalt microbeam [37]- [39] is routinely used for the irradiation of living cells using an extremely wide range of LET values-3-200 keV/µm (protons at 1-20 MeV or helium nuclei at 1-28 MeV). The beam diameter is 2 µm FWHM, achieved by focusing the beam from either a 3.75 MV Van de Graaff generator or a variable energy cyclotron, using an RF ion source and a penning ion source, respectively [40].…”

Section: Ptb (Braunschweig Germany)mentioning

confidence: 99%

Single-Particle/Single-Cell Ion Microbeams as Probes of Biological Mechanisms

Bigelow

Brenner

Garty

et al. 2008

IEEE Trans. Plasma Sci.

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Abstract-An ion microbeam is a very narrow beam of charged particles, typically protons, alpha particles, or heavier, of micrometer/submicrometer size, corresponding to cellular/ subcellular dimensions. Together with integrated techniques for locating live cellular or subcellular targets, they allow rapid sequential irradiation of these targets. This review covers both the technology involved in modern single-cell microbeams, as well as some current applications. The recent explosion of interest in microbeams was initially driven by interest in the domestic radon problem, in which target cells are exposed either to zero or one alpha particle. Microbeams allow cells to be individually irradiated with exact numbers of particles. As microbeams were built, refined, and used, the biological questions that were addressed with them have considerably broadened, to encompass many aspects of damage signal transduction. Two areas in particular have attracted much interest: One is the use of microbeams to address the sensitivity of subcellular targets, such as the cytoplasm or mitochondria. The other reflects the ability of the microbeam to irradiate some cells, but not others, allowing a direct investigation of the so-called bystander effect, where signals from irradiated cells can apparently cause biological responses in neighboring unirradiated cells.

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The PTB single ion microbeam for irradiation of living cells

Cited by 66 publications

References 10 publications

Geant4-DNA simulation of DNA damage caused by direct and indirect radiation effects and comparison with biological data.

Geant4-DNA simulation of DNA damage caused by direct and indirect radiation effects and comparison with biological data.

At the Tip of an MeV Beam: Provoking Cells and Performing Tomographic Imaging

Single-Particle/Single-Cell Ion Microbeams as Probes of Biological Mechanisms

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