The pss4 gene from Rhizobium leguminosarum bv viciae VF39: cloning, sequence and the possible role in polysaccharide production and nodule formation

Ивашина, Т. В.; Mi, Khmelnitsky; Mg, Shlyapnikov; Kanapin, Alexander; Vn, Ksenzenko

doi:10.1016/0378-1119(94)90868-0

Cited by 47 publications

(62 citation statements)

References 25 publications

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“…EPS-deficient mutant strains of R. leguminosarum bvs. trifolii and viciae, and Sinorhizobium meliloti induce formation of empty or only partially infected nodulelike structures on roots of compatible host plants that are ineffective in nitrogen fixation (Ivashina et al 1994;Cheng and Walker 1998;Janczarek and Rachwał 2013). On the other hand, EPS-overproducing R. leguminosarum and S. meliloti strains are characterized by significantly enhanced occupancy of the host plant nodules and symbiotic effectiveness (Janczarek et al 2009;Lehman and Long 2013).…”

Section: Introductionmentioning

confidence: 99%

Production of exopolysaccharide by Rhizobium leguminosarum bv. trifolii and its role in bacterial attachment and surface properties

et al. 2014

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Background and aims The acidic exopolysaccharide (EPS) produced by Rhizobium leguminosarum bv. trifolii is required for the establishment of effective symbiosis with compatible host plants (Trifolium spp.). In the rhizobium-legume interaction, early stages of root infection and nodule development have been well studied from a genetic standpoint. However, factors important for colonization of several surfaces by rhizobia, including soil particles and roots, have not yet been thoroughly investigated. The aim of this study was establishing of environmental factors affecting production of EPS by R. leguminosarum bv. trifolii strain 24.2 and the role of this polysaccharide in bacterial surface properties and attachment ability. Methods Besides the wild-type strain, its derivatives differing in the level of EPS produced were used to these analyses. The ability of attachment to abiotic and biotic surfaces of these strains were established using CFU counting experiments. Three-dimensional structure and other parameters of biofilms formed were characterized in confocal laser scanning microscopy. Electrokinetic (zeta) potential of rhizobial cells were determined using Laser Doppler Velocimetry. Results It was evidenced that the ability of R. leguminosarum bv. trifolii to produce EPS significantly affected bacterial attachment and biofilm formation on both abiotic and biotic surfaces. In addition, the presence of this polysaccharide influenced the zeta potential of rhizobial cells. Mutant strains having a mutation in genes involved in EPS synthesis were significantly impaired in attachment, whereas strains overproducing this polysaccharide showed higher adhesion efficiency to all of the tested materials. EPS facilitated attachment of bacterial cells to the tested surfaces most probably due to hydrophobic interactions and heterogeneity of the envelope surface. Conclusions EPS produced by R. leguminosarum bv. trifolii plays a significant role in attachment and biofilm formation to both abiotic and biotic surfaces as well as bacterial surface properties.

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Section: Introductionmentioning

confidence: 99%

Production of exopolysaccharide by Rhizobium leguminosarum bv. trifolii and its role in bacterial attachment and surface properties

et al. 2014

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“…The open reading frames (orfs) interrupted by insertion of the Tn5 transposon were named as pss (polysaccharide synthesis) according to Borthakur and coworkers [44]. The Tn5 insertion in the GL4 mutant was localized within the pssA gene [45], the ortholog of which was previously identified in Rlp 8002 [44]. The mutations in GL2 and GL6 were mapped within the pssE and pssD genes, respectively.…”

Section: Organization Of Exopolysaccharide Biosynthesis Genesmentioning

confidence: 99%

“…The gene was shown to be transcribed as a monocistronic mRNA [45]. Upstream of the pssA is the pssB gene encoding inositol monophosphatase [49][50][51].…”

Section: Organization Of Exopolysaccharide Biosynthesis Genesmentioning

confidence: 99%

“…Members of this family catalyze the formation of a phosphodiester bond between polyprenol phosphate and hexoso-1-phosphate, which is donated by nucleotide sugars. The pssA gene is highly conserved in R. leguminosarum biovars and R. etli [44][45][46]50]. The pssA mutants do not produce EPS and as a consequence impaired the normal development of the nitrogen-fixing nodules on the appropriate plant hosts and the formation of biofilms both in vitro and on root hairs [5,45,46,50].…”

Section: Assembly Of the Eps Repeating Unitmentioning

confidence: 99%

“…The pssA gene is highly conserved in R. leguminosarum biovars and R. etli [44][45][46]50]. The pssA mutants do not produce EPS and as a consequence impaired the normal development of the nitrogen-fixing nodules on the appropriate plant hosts and the formation of biofilms both in vitro and on root hairs [5,45,46,50]. Some contradictory results exist concerning the influence of pssA mutations on the synthesis of CPS which displays similar structure to EPS in terms of glycosyl composition [5,30,62].…”

Section: Assembly Of the Eps Repeating Unitmentioning

confidence: 99%

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Exopolysaccharide Biosynthesis in Rhizobium leguminosarum: From Genes to Functions

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Exopolysaccharide Production in Rhizobia Is Regulated by Environmental Factors

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The pss4 gene from Rhizobium leguminosarum bv viciae VF39: cloning, sequence and the possible role in polysaccharide production and nodule formation

Cited by 47 publications

References 25 publications

Production of exopolysaccharide by Rhizobium leguminosarum bv. trifolii and its role in bacterial attachment and surface properties

Production of exopolysaccharide by Rhizobium leguminosarum bv. trifolii and its role in bacterial attachment and surface properties

Exopolysaccharide Biosynthesis in Rhizobium leguminosarum: From Genes to Functions

Exopolysaccharide Production in Rhizobia Is Regulated by Environmental Factors

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