Exopolysaccharide Biosynthesis in Rhizobium leguminosarum: From Genes to Functions

Ивашина, Т. В.; Ksenzenko, V. N.

doi:10.5772/51202

Cited by 8 publications

(2 citation statements)

References 105 publications

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“…EPS biosynthesis is unknown in most of the inoculants authorized in Brazil (Supplementary Table 1), particularly in Bradyrhizobium strains, however, it has been well-studied in Sinorhizobium meliloti and Rhizobium leguminosarum and required exo, exs, exp, and muc genes, which are mainly grouped and conserved in different strains (Janczarek et al, 2015). They are distributed on the chromosome or in symbiotic plasmids (Ivashina and Ksenzenko, 2012;Ormeño-Orrillo et al, 2012). EPS contain mostly D-glucose and D-galactose (Castellane et al, 2014(Castellane et al, , 2017(Castellane et al, , 2018(Castellane et al, , 2019, uronic acids and non-sugar substitutions can also be very important (López-Baena et al, 2016).…”

Section: Importance Of Exopolysaccharides Production In Symbiosismentioning

confidence: 99%

Rhizobial Exopolysaccharides and Type VI Secretion Systems: A Promising Way to Improve Nitrogen Acquisition by Legumes

et al. 2021

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At present, there are numerous examples in which symbiotic nitrogen fixation by rhizobia can totally replace the use of nitrogen fertilizers in legume crops. Over the years, there has been a great effort by research institutions to develop and select rhizobial inoculants adapted for these crops. The symbiotic process is highly dependent on the dynamic exchange of signals and molecular nutrients between partners. Our focus in this review was to discuss the two key determinants in successful symbiotic interactions of rhizobia to nodulate pulses. One of them is the production of exopolysaccharides (EPS) and the other the presence of the type VI secretion system (T6SS). EPS are extracellular polymers weakly associated with the bacterial surface and are abundantly released into acid soils facilitating, among other functions, an adaptation of rhizobia to this environment. On the other hand, different protein secretion systems, involved in symbiosis, have been described in rhizobia. This is not the case with the T6SS. The current availability of various rhizobial genomes offers the possibility of discussing its role in symbiosis. The study of these determinants will be of great utility for the selection of effective inoculants for legumes, a promising way to improve nitrogen acquisition by legumes.

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Section: Importance Of Exopolysaccharides Production In Symbiosismentioning

confidence: 99%

Rhizobial Exopolysaccharides and Type VI Secretion Systems: A Promising Way to Improve Nitrogen Acquisition by Legumes

et al. 2021

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“…Therefore, transferring EPS gene cluster into P. fluorescens, extra characteristics might be added to the P. fluorescens bacteria since EPS gene cluster has ability to express vital enzymes. For example, this gene cluster is capable to produce biofilm layer on the surfaces of the cells which have ability to protect cellular against environmental stress, nutrient aggregations for the plants and enhance surface attachment of the nutrients for the roots (Tanya et al, 2012). Additionally, symbiotic signaling for the molecules can be enhanced by extracellular polymers which can adjust the responses of the plants during infections, as chemically extracellular polysaccharides has special structural recognitions and physic-chemical possessions (Fraysse et al, 2003).…”

Section: Molecular Identifications Of Bacteria and Eps Gene Clustermentioning

confidence: 99%

Transferring of Exopolysaccharide (EPS) Gene Cluster from Rhizobium leguminosarum to Pseudomonas fluorescens by Horizontal Gene Transfer Techniques

Mahmud

Khudhur

2021

ZJPAS

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To increase drought resistance of efficient plant growth promoting Psuedomonas fluorescens isolated from Erbil governorate soils, exopolysaccharide (EPS) gene cluster was transferred from Rhizobium leguminosarum to P. fluorescens. EPS gene cluster is responsible for generating of capsular polysaccharides which are tightly attached to the cell surface and extracellular polysaccharides that are directly secreted to the environment. For P. fluorescens and R. leguminosarum isolations, different soil samples were collected in Erbil governorate. According to morphological, biochemical and molecular examinations 75 P. fluorescens isolates and 58 R. leguminosarum isolates were identified from 110 soil samples. Most P. fluorescens isolates showed efficiency in phosphate solubilization, hydrogen cyanide, siderophores and indol acidic acid production, the most efficient P. fluorescens (Mpf16) was selected for conjugation process. Potential transferring of EPS genes, included EPS1, EPS2, and EPS3, was investigated in this study. Gene cluster was transferred successfully from R. leguminosarum into P. fluorescens by horizontal conjugation gene transfer technique after confirmation via molecular techniques using different primers and enzymes. The expression of transferred EPS gene cluster from transconjugant P. fluorescens was confirmed by testing capsule formations from viable cells. The new generated capsule former P. fluorescens can be used later on as an efficient bio-fertilizer agent for enhancing plant growth and crop production in drought cultivated soils.

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Flocculating, emulsification and metal sorption properties of a partial characterized novel exopolysaccharide produced by Rhizobium tropici SRA1 isolated from Psophocarpus tetragonolobus (L) D.C.

et al. 2018

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Exopolysaccharide Biosynthesis in Rhizobium leguminosarum: From Genes to Functions

Cited by 8 publications

References 105 publications

Rhizobial Exopolysaccharides and Type VI Secretion Systems: A Promising Way to Improve Nitrogen Acquisition by Legumes

Rhizobial Exopolysaccharides and Type VI Secretion Systems: A Promising Way to Improve Nitrogen Acquisition by Legumes

Transferring of Exopolysaccharide (EPS) Gene Cluster from Rhizobium leguminosarum to Pseudomonas fluorescens by Horizontal Gene Transfer Techniques

Flocculating, emulsification and metal sorption properties of a partial characterized novel exopolysaccharide produced by Rhizobium tropici SRA1 isolated from Psophocarpus tetragonolobus (L) D.C.

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