The pseudogenes of eukaryotic translation elongation factors (EEFs): Role in cancer and other human diseases

Cristiano, Luigi

doi:10.1016/j.gendis.2021.03.009

Cited by 17 publications

(6 citation statements)

References 121 publications

(269 reference statements)

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“…Protein interaction network analysis of differentially expressed proteins identified elongation factor 1-gamma (eEF1G) as the most interacting protein. eEF1G is a subunit of the eEF1 complex that plays a role in the transport of AmAcyl-tRNAs to the ribosomes for protein synthesis [68]. The N-terminal region of the eEF1G protein contains a glutathione transferase domain, which may be involved in the regulation of the assembly of multi-subunit complexes containing eEF1G and AmAcyl-tRNA synthetase.…”

Section: Discussionmentioning

confidence: 99%

Construction of dental pulp decellularized matrix by cyclic lavation combined with mechanical stirring and its proteomic analysis

Zhang,

Bi,

Huang

et al. 2024

Biomed. Mater.

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The decellularized matrixhas a great potential for tissue remodeling and regeneration; however, decellularization could induce host immune rejection due to incomplete cell removal or detergent residues, thereby posing significant challenges for its clinical application. Therefore, the selection of an appropriate detergent concentration, further optimization of tissue decellularization technique, increased of biosafety in decellularized tissues, and reduction of tissue damage during the decellularization procedures are pivotal issues that need to be investigated. Inthis study, we tested several conditions and determined that 0.1% Sodium dodecyl sulfate(SDS) and three decellularization cycles were the optimal conditions for decellularization of pulp tissue. Decellularization efficiency was calculated and the preparation protocol for dental pulp decellularization matrix (DPDM) was further optimized. To characterize the optimized DPDM, the microstructure, odontogenesis-related protein and fiber content were evaluated. Our results showed that the properties of optimized DPDM were superior to those of the non-optimized matrix. We also performed the 4D-Label-free quantitative proteomic analysis of DPDM and demonstrated the preservation of proteins from the natural pulp. This study provides a solid theoretical and experimental foundation for the potential application of DPDM in pulp regeneration.

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Section: Discussionmentioning

confidence: 99%

Construction of dental pulp decellularized matrix by cyclic lavation combined with mechanical stirring and its proteomic analysis

Zhang,

Bi,

Huang

et al. 2024

Biomed. Mater.

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“…EEF1D has also been reported to be overexpressed in various tumors, such as glioblastoma, glioma, and lymphoma ( 23 – 26 ). Additionally, there are eight pseudogenes of EEF1D in the human genome, and EEF1D pseudogene-derived proteins or short peptides have been reported to be correlated with a range of tumors and neurological diseases, such as breast carcinoma, ankylosing spondylitis, non-small cell lung cancer, and lymphoma ( 27 ).…”

Section: Discussionmentioning

confidence: 99%

Autoantibodies against eukaryotic translation elongation factor 1 delta in two patients with autoimmune cerebellar ataxia

Guo,

Ren,

Fan

et al. 2024

Front. Immunol.

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BackgroundAutoantibodies are useful biomarkers for the early detection and diagnosis of autoimmune cerebellar ataxia (ACA).ObjectiveTo identify novel autoantibody candidates in ACA patients.MethodsPatients with cerebellar ataxia of unknown cause were recruited from July 2018 to February 2023. Anti-neural autoantibodies in patient samples were detected by tissue-based indirect immunofluorescence assay (TBA) on rat cerebellum sections. TBA-positive samples were further screened for well-established anti-neural autoantibodies using commercial kits. Tissue-immunoprecipitation (TIP) and subsequent mass spectrometric (MS) analysis were used to explore the target antigens of autoantibodies in samples that were TBA-positive but negative for known autoantibodies. The specific binding between autoantibodies and the identified target antigen was confirmed by neutralization experiments, recombinant cell-based indirect immunofluorescence assay (CBA), and western blotting experiments.ResultsThe eukaryotic translation elongation factor 1 delta (EEF1D) protein was identified as a target antigen of autoantibodies in samples from a 43-year-old female ACA patient, while the specific binding of autoantibodies and EEF1D was confirmed by subsequent experiments. A second anti-EEF1D autoantibody-positive ACA patient, a 59-year-old female, was detected in simultaneous screening. The main clinical manifestations in each of the two patients were cerebellar syndrome, such as unsteady walking and limb ataxia. Both patients received immunotherapy, including corticosteroids, intravenous immunoglobulin, and mycophenolate mofetil. Their outcomes provided evidence to support the effectiveness of immunotherapy, but the cerebellar atrophy that occurred before treatment may be irreversible.ConclusionIn the current study, we identified anti-EEF1D autoantibody as a novel autoantibody candidate in ACA. Its pathological roles and diagnostic value need to be further verified in larger-scale studies.

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“…EEF1D has also been reported overexpression in various tumors, such as glioblastoma, glioma, lymphoma (11-14). Additionally, there are eight pseudogenes of EEF1D in human genome, EEF1D pseudogene-derived proteins or short peptides were reported correlated to a range of tumors and neurological diseases, such as breast carcinoma, ankylosing spondylitis, non-small cell lung cancer, lymphoma (15).…”

Section: Discussionmentioning

confidence: 99%

Autoantibodies against eukaryotic translation elongation factor 1 delta (EEF1D) in two patients with autoimmune cerebellar ataxia

Guo,

Ren,

Fan

et al. 2023

Preprint

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To report a novel autoantibody against eukaryotic translation elongation factor 1 delta (EEF1D) in two patients with autoimmune cerebellar ataxia (ACA). The patients on one center with cerebellar ataxia of unknown cause, who were tested positive with tissue-based indirect immunofluorescence assay (TBA) on rat cerebellum sections and negative for comprehensive anti-neural autoantibodies panel, were investigated for novel autoantibody identification. Tissue-immunoprecipitation (TIP) combined with mass spectrometric (MS) analysis was used to identify the target antigen. The EEF1D protein was identified from complex precipitated by serum and cerebrospinal fluid (CSF) of one patient, while the specific binding of autoantibodies and EEF1D were confirmed by subsequent neutralization experiment, recombinant cell-based indirect immunofluorescence assay (CBA) and western blot. In consequently test using CBA, another anti-EEF1D positive ACA patient was identified and confirmed by western blot. All enrolled 30 health doners and 15 connective tissue diseases patients without neurological disorders were anti-EEF1D negative. The two anti-EEF1D positive patients presented similar clinical manifestations, their outcomes supported the effectiveness of immunotherapy, but the cerebellar atrophy occurred before treatment may be irreversible. Symptoms of one patient worsened again after the weaning of pulse corticosteroid therapy. The identification of anti-EEF1D autoantibody provides a new potential biomarker for early diagnosis and recurrence prevention of ACA.

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The pseudogenes of eukaryotic translation elongation factors (EEFs): Role in cancer and other human diseases

Cited by 17 publications

References 121 publications

Construction of dental pulp decellularized matrix by cyclic lavation combined with mechanical stirring and its proteomic analysis

Construction of dental pulp decellularized matrix by cyclic lavation combined with mechanical stirring and its proteomic analysis

Autoantibodies against eukaryotic translation elongation factor 1 delta in two patients with autoimmune cerebellar ataxia

Autoantibodies against eukaryotic translation elongation factor 1 delta (EEF1D) in two patients with autoimmune cerebellar ataxia

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