The proteome profiles of the olfactory bulb of juvenile, adult and aged rats - an ontogenetic study

Abstract

BackgroundIn this study, we searched for proteins that change their expression in the olfactory bulb (oB) of rats during ontogenesis. Up to now, protein expression differences in the developing animal are not fully understood. Our investigation focused on the question whether specific proteins exist which are only expressed during different development stages. This might lead to a better characterization of the microenvironment and to a better determination of factors and candidates that influence the differentiation of neuronal progenitor cells.ResultsAfter analyzing the samples by two-dimensional polyacrylamide gel electrophoresis (2DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), it could be shown that the number of expressed proteins differs depending on the developmental stages. Especially members of the functional classes, like proteins of biosynthesis, regulatory proteins and structural proteins, show the highest differential expression in the stages of development analyzed.ConclusionIn this study, quantitative changes in the expression of proteins in the oB at different developmental stages (postnatal days (P) 7, 90 and 637) could be observed. Furthermore, the expression of many proteins was found at specific developmental stages. It was possible to identify these proteins which are involved in processes like support of cell migration and differentiation.Electronic supplementary materialThe online version of this article (doi:10.1186/s12953-014-0058-x) contains supplementary material, which is available to authorized users.

Differential Proteomics of the Cerebral Cortex of Juvenile, Adult and Aged Rats: An Ontogenetic Study

“…The protein is downregulated at P7 in the Cx. In previous studies, an upregulation of this protein could be determined at P7 in other regions of the brain (olfactory bulb, cerebellum) [31,67]. Also Gurniak et al [68] show that a high abundance of this protein is essential at the early levels of development.…”

“…The extraction of proteins was performed according to published standardized protocols [26,30,31]. In the following the steps are described shortly.…”

“…Only those spots were considered to be significantly up-or downregulated that showed a SVQ (Spot Volume Quotient) of 0.6 or less and 1.67 or greater [26,31,34]. The differences were evaluated significantly if differentially expressed spots were detected in at least four gel images (correlated spots) belonging to one test group.…”

Olfactory bulb proteome dynamics during the progression of sporadic Alzheimer's disease: identification of common and distinct olfactory targets across Alzheimer-related co-pathologies

“…For several decades, neuroanatomical, volumetric, and histological approaches have been the gold standard techniques employed to characterize the OB functionality. However, little attention has been focused specifically on the molecular composition of the OB from the perspective of proteomics [ 13 - 15 ]. We consider that deciphering the progressive proteome-wide alterations that occurs in the OB derived from AD cases with different Braak staging, might help develop early diagnosis and identify potential therapeutic targets for AD.…”

Membrane Protein Identification in Rodent Brain Tissue Samples and Acute Brain Slices

“…Two-dimensional gel electrophoresis was performed as previously described [15,16]. In brief, for the first dimension, the samples were diluted with rehydration buffer (6 M urea, 2 M thiurea, 32.5 mM CHAPS hydrate, 16.2 mM dithiothreitol (DTT), 2.5% ampholytes (Biochemika, 39878)).…”