Differential Proteomics of the Cerebral Cortex of Juvenile, Adult and Aged Rats: An Ontogenetic Study

Proteome Profiling of Rat Brain Cortical Changes during Early Postnatal Brain Development

“…This work demonstrated a cross-study PND proteomic data comparison between the current rat cortex work (P2, P8, P15, and P22) and the three other past studies: the mouse cortex synaptosomal fraction (P9, P15, and P21), 1 the rat cortex synaptosomal and mitochondrial fractions (P1, P10, and P20), 5 and the rat cortex proteomic study involving 2D-PAGE gel analysis (P7 and P90). 4 The synaptic and mitochondrial protein PND ratios obtained from the current rat cortex work correlated well with the similar PND ratios obtained from the rat cortex synaptosomal and mitochondrial cellular fractions. 5 An excellent correlation was found in this work between the male and female rat cortex proteomes during the early PND, P2−P22.…”

“…Cerebral cortex samples obtained from the rat PND points P7, P90, and P637 were analyzed by two-dimensional polyacrylamide gel electrophoresis (2DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) . Seventy-three proteins, which were described in the 2D-PAGE study in Table 1 in ref as either up- or down-regulated between P7 and P90, were also identified in the current rat work.…”

“…Cerebral cortex samples obtained from the rat PND points P7, P90, and P637 were analyzed by two-dimensional polyacrylamide gel electrophoresis (2DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) . Seventy-three proteins, which were described in the 2D-PAGE study in Table 1 in ref as either up- or down-regulated between P7 and P90, were also identified in the current rat work. Despite the significant difference in time between the reference points P22 and P90, 85% of the reported proteins agreed in the + or – expression direction sign represented by the log 2 (P8/P22) ratio (current work) and the P7/P90 up- or down-regulation direction.…”

“…Most CV% values were found within the 0–20% range, and the detailed CV% distribution across sample groups was as follows: (a) CV% within 0–20% for the following: females, P2: 71.9%, P8: 79.6%, P15: 80%, P22: 78%; males: P2: 72.5%, P8: 81.5%, P15: 71.9%, P22: 70.8%; (b) CV% within 20–40% for the following: females, P2: 19.2%, P8: 14.2%, P15: 14.2%, P22: 15%; males: P2: 19.4%, P8: 14.1%, P15: 18.9%, P22: 19.4%; (c) CV% within 40–60% for the following: females, P2: 6.3%, P8: 4.1%, P15: 3.9%, P22: 3.8%; males: P2: 5.4%, P8: 2.9%, P15: 5.9%, P22: 5.9%; (d) CV% within 60–100% for the following: females, P2: 2.5%, P8: 1.9%, P15: 1.6%, P22: 2.2%; males: P2: 2.2%, P8: 1.3%, P15: 2.5%, P22: 2.8%; (e) CV% above 100% for the following: females, P2: 0.20%, P8: 0.20%, P15: 0.27%, P22: 0.16%; males: P2: 0.43%, P8: 0.20%, P15: 0.82%, P22: 1.06%, where the listed percent values following the PND symbols represent the percentage of proteins quantified in a given male and female category falling within the protein abundance CV% range. The logarithmic ratios, abundance ratio (log 2 ) (female, P X )/(female, P 22 ) and abundance ratio (log 2 ) (male, P X )/(male, P 22 ), x = 2, 8, 15, and 22, and the corresponding adjusted p -values generated by PDv.2.5 were used for the data comparison with the datasets available from refs , , and . Consequently, the mouse cortex synaptosome-fraction data from Supplementary Table 1 in ref were re-normalized from P280 to P21 by subtracting the Excel columns H from F and H from G: log 2 (P n /P280) – log 2 (P21/P280) = log 2 (P n /P21), where n = 9 and 15.…”

“…During the perinatal period, environmental stressors pose a potential threat to brain development because the brain undergoes extensive, rapid developmental and organizational changes. − Biological and environmental stresses and insults sustained during this period might negatively impact brain development . Therefore, to be able to better assess the impact of a chemical exposure on developing brain, it is important to gather information about the biological transformations occurring during postnatal development of the animals used in toxicoproteomic studies.…”