The protein phosphatase 2A represents a novel cellular target for hepatitis C virus NS5A protein

Georgopoulou, Urania; Tsitoura, Panagiota; Kalamvoki, Maria; Mavromara, Penelope

doi:10.1016/j.biochi.2005.12.003

Cited by 33 publications

(26 citation statements)

References 66 publications

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“…Hepatitis C and insulin resistance IR is protein phosphatase 2A (PP2A). PP2A can affect several cell pathways and is upregulated in HCV infection, possibly due to increased endoplasmic reticulum (ER) stress [81] or directly by the HCV non-structural protein NS5A [82] (Figure 1). PP2A has been shown to mediate HCV-associated IR by dephosphorylating and thus inactivating Akt [83] .…”

Section: Hcv Ns5amentioning

confidence: 99%

Molecular mechanisms of insulin resistance in chronic hepatitis C

Douglas

George²

2009

WJG

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It is now widely recognized that chronic hepatitis C (CHC) is associated with insulin resistance (IR) and type 2 diabetes, so can be considered a metabolic disease. IR is most strongly associated with hepatitis C virus (HCV) genotype 1, in contrast to hepatic steatosis, which is associated with genotype 3 infection. Apart from the well-described complications of diabetes, IR in CHC predicts faster progression to fibrosis and cirrhosis that may culminate in liver failure and hepatocellular carcinoma. More recently, it has been recognized that IR in CHC predicts a poor response to antiviral therapy. The molecular mechanisms for the association between IR and HCV infection are not well defined. This review will elaborate on the clinical associations between CHC and IR and summarize current knowledge regarding the molecular mechanisms that potentially mediate HCV-associated IR.

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Section: Hcv Ns5amentioning

confidence: 99%

Molecular mechanisms of insulin resistance in chronic hepatitis C

Douglas

George²

2009

WJG

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“…The GFP-ERF plasmid [13] was used for transient expression of ERF, and transfection of WRL68, Huh7 and BHK21 cells was performed as previously described [14] or with jetPei (Poluplus-transfection). A minimum of 100 transfected cells were counted for each condition and 50 cells for each fraction quantity, in at least two independent wells.…”

Section: Plasmidsmentioning

confidence: 99%

Evidence for cellular uptake of recombinant hepatitis C virus non‐enveloped capsid‐like particles

et al. 2007

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Although the hepatitis C virus (HCV) is an enveloped virus, naked nucleocapsids have been reported in the serum of infected patients, and most recently novel HCV subgenomes with deletions of the envelope proteins have been identified. However the significance of these findings remains unclear. In this study, we used the baculovirus expression system to generate recombinant HCV capsid-like particles, and investigated their possible interactions with cells. We show that expression of HCV core in insect cells can sufficiently direct the formation of capsid-like particles in the absence of the HCV envelope glycoproteins and of the 5 0 untranslated region. By confocal microscopy analysis, we provide evidence that the naked capsid-like particles could be uptaken by human hepatoma cells. Moreover, our findings suggest that they have the potential to produce cell-signaling effects.

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“…Accordingly, we found early up-regulation of PP2A scaffold subunit A and subsequent dephosphorylation of Tyr-307 in the catalytic subunit, suggesting PP2A activation in Huh7 infected cells (29,51). Different viruses target PP2A in order to deregulate selected cellular pathways in the host and promote viral progeny (52) including human cytomegalovirus (53), parainfluenza virions (54), adenovirus (55)(56)(57), human immunodeficiency virus (58), hepatitis C virus (59,60), and simian virus 40 and polyomavirus (61). Activation of PP2A is induced by hepatitis viruses through mechanisms dependent on endoplasmic reticulum calcium release (59).…”

Section: Fig 5 Cgalmentioning

confidence: 99%

Identification of Replication-competent HSV-1 Cgal+ Strain Signaling Targets in Human Hepatoma Cells by Functional Organelle Proteomics

Santamaría

Mora

Potel

et al. 2009

Molecular & Cellular Proteomics

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In the present work, we have attempted a comprehensive analysis of cytosolic and microsomal proteomes to elucidate the signaling pathways impaired in human hepatoma (Huh7) cells upon herpes simplex virus type 1 (HSV-1; Cgal ؉ ) infection. Using a combination of differential in-gel electrophoresis and nano liquid chromatography/tandem mass spectrometry, 18 spots corresponding to 16 unique deregulated cellular proteins were unambiguously identified, which were involved in the regulation of essential processes such as apoptosis, mRNA processing, cellular structure and integrity, signal transduction, and endoplasmic-reticulum-associated degradation pathway. Based on our proteomic data and additional functional studies target proteins were

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The protein phosphatase 2A represents a novel cellular target for hepatitis C virus NS5A protein

Cited by 33 publications

References 66 publications

Molecular mechanisms of insulin resistance in chronic hepatitis C

Molecular mechanisms of insulin resistance in chronic hepatitis C

Evidence for cellular uptake of recombinant hepatitis C virus non‐enveloped capsid‐like particles

Identification of Replication-competent HSV-1 Cgal+ Strain Signaling Targets in Human Hepatoma Cells by Functional Organelle Proteomics

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