Identification of Replication-competent HSV-1 Cgal+ Strain Signaling Targets in Human Hepatoma Cells by Functional Organelle Proteomics

Santamaría, Enrique; Mora, María I.; Potel, Corinne; Fernández‐Irigoyen, Joaquín; Carro-Roldán, Elvira; Hernández-Alcoceba, Rubén; Prìeto, Jesús; Epstein, Alberto L.; Corrales, Fernando J.

doi:10.1074/mcp.m800202-mcp200

Cited by 19 publications

(28 citation statements)

References 87 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Upregulation of PP2A scaffold subunit A and subsequent dephosphorylation of Tyr-307 in the catalytic subunit was found, suggesting PP2A activation in Huh7 infected cells [51,52]. Activation of serine-threonine PP2A was found in Huh7 cells upon HSV-1 infection, and PP2A activation paralleled dephosphorylation and inactivation of the downstream mitogen-activated protein (MAP) kinase pathway [53]. In this study, the up-regulation of PP2A was found in TGEV infected ST cells, suggesting PP2A plays an important role in the dephosphorylation of cellular and viral protein during TGEV infection.…”

Section: Discussionmentioning

confidence: 99%

Identification of cellular proteome using two-dimensional difference gel electrophoresis in ST cells infected with transmissible gastroenteritis coronavirus

et al. 2013

View full text Add to dashboard Cite

BackgroundTransmissible gastroenteritis coronavirus (TGEV) is an enteropathogenic coronavirus that causes diarrhea in pigs, which is correlated with high morbidity and mortality in suckling piglets. Information remains limited about the comparative protein expression of host cells in response to TGEV infection. In this study, cellular protein response to TGEV infection in swine testes (ST) cells was analyzed, using the proteomic method of two-dimensional difference gel electrophoresis (2D DIGE) coupled with MALDI-TOF-TOF/MS identification.Results33 differentially expressed protein spots, of which 23 were up-regulated and 10 were down-regulated were identified. All the protein spots were successfully identified. The identified proteins were involved in the regulation of essential processes such as cellular structure and integrity, RNA processing, protein biosynthesis and modification, vesicle transport, signal transduction, and the mitochondrial pathway. Western blot analysis was used to validate the changes of alpha tubulin, keratin 19, and prohibitin during TGEV infection.ConclusionsTo our knowledge, we have performed the first analysis of the proteomic changes in host cell during TGEV infection. 17 altered cellular proteins that differentially expressed in TGEV infection were identified. The present study provides protein-related information that should be useful for understanding the host cell response to TGEV infection and the underlying mechanism of TGEV replication and pathogenicity.

show abstract

Section: Discussionmentioning

confidence: 99%

Identification of cellular proteome using two-dimensional difference gel electrophoresis in ST cells infected with transmissible gastroenteritis coronavirus

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Post-transcriptional regulation, such as alternative splicing, delayed translation, and mRNA degradation, can all affect the protein level that results from a given transcriptional output. While proteomic analyses have not yet been applied to infected neurons, early results from alternative models (Loret et al 2008; Skiba et al 2008; Santamaria et al 2009) suggest that these techniques will likely be fruitful for gaining comprehensive data about proteins levels in infected cells.…”

Section: Future Directionsmentioning

confidence: 99%

A Common Neuronal Response to Alphaherpesvirus Infection

Szpara

Kobiler

Enquist

2010

J Neuroimmune Pharmacol

View full text Add to dashboard Cite

Alphaherpesviruses are a subfamily of the Herpesviridae that can invade the nervous system and establish either lytic or latent infections. The establishment of latent infection can occur only in neurons, indicating a unique virus–host interaction in these cells. Here, we compare results from seven microarray studies that focused on the host response of either neural tissue or isolated neurons to alphaherpesvirus infection. These studies utilized either herpes simplex virus type 1 or pseudorabies virus as the infectious agent. From these data, we have found common host responses spanning a variety of infection models in different species, with different herpesvirus strains, and during all phases of infection including lytic, latent, and reactivation. The repeated observation of transcriptional effects on these genes and gene families indicates their likely importance in host defenses or the viral infectious process. We discuss the possible role of these different genes and genes families in alphaherpesvirus infection.

show abstract

“…In addition, many viruses also induce general changes in the protein composition of infected cells; for example, proteomic changes may occur as a result of the inhibition of cellular macromolecule synthesis or the activation of host antiviral responses. These changes can be monitored by various approaches, including quantitative proteomics, which has been used to characterize changes in the total proteome of CHIKV-infected cells (27) or the nucleolar proteome in coronavirus-or influenza virus-infected avian cells (28,29), to analyze changes in endoplasmic reticulum-mitochondrion contacts induced by human cytomegalovirus infection (30), to elucidate the signaling pathways impaired in human hepatoma cells during herpes simplex virus type 1 infection (31), and to identify host proteins associated with picornavirus RNAs (32). The identification of relocalized host cell components and/or host cell components with altered quantities during infection is especially important for understanding positive-strand RNA viruses.…”

mentioning

confidence: 99%

Magnetic Fractionation and Proteomic Dissection of Cellular Organelles Occupied by the Late Replication Complexes of Semliki Forest Virus

Varjak

Saul

Arike

et al. 2013

J Virol

View full text Add to dashboard Cite

Alphavirus replicase complexes are initially formed at the plasma membrane and are subsequently internalized by endocytosis. During the late stages of infection, viral replication organelles are represented by large cytopathic vacuoles, where replicase complexes bind to membranes of endolysosomal origin. In addition to viral components, these organelles harbor an unknown number of host proteins. In this study, a fraction of modified lysosomes carrying functionally intact replicase complexes was obtained by feeding Semliki Forest virus (SFV)-infected HeLa cells with dextran-covered magnetic nanoparticles and later magnetically isolating the nanoparticle-containing lysosomes. Stable isotope labeling with amino acids in cell culture combined with quantitative proteomics was used to reveal 78 distinct cellular proteins that were at least 2.5-fold more abundant in replicase complex-carrying vesicles than in vesicles obtained from noninfected cells. These host components included the RNA-binding proteins PCBP1, hnRNP M, hnRNP C, and hnRNP K, which were shown to colocalize with the viral replicase. Silencing of hnRNP M and hnRNP C expression enhanced the replication of SFV, Chikungunya virus (CHIKV), and Sindbis virus (SINV). PCBP1 silencing decreased SFV-mediated protein synthesis, whereas hnRNP K silencing increased this synthesis. Notably, the effect of hnRNP K silencing on CHIKV-and SINV-mediated protein synthesis was opposite to that observed for SFV. This study provides a new approach for analyzing the proteome of the virus replication organelle of positive-strand RNA viruses and helps to elucidate how host RNA-binding proteins exert important but diverse functions during positive-strand RNA viral infection.

show abstract

Identification of Replication-competent HSV-1 Cgal+ Strain Signaling Targets in Human Hepatoma Cells by Functional Organelle Proteomics

Cited by 19 publications

References 87 publications

Identification of cellular proteome using two-dimensional difference gel electrophoresis in ST cells infected with transmissible gastroenteritis coronavirus

Identification of cellular proteome using two-dimensional difference gel electrophoresis in ST cells infected with transmissible gastroenteritis coronavirus

A Common Neuronal Response to Alphaherpesvirus Infection

Magnetic Fractionation and Proteomic Dissection of Cellular Organelles Occupied by the Late Replication Complexes of Semliki Forest Virus

Contact Info

Product

Resources

About