The protective role ofTropaeolum majuson blood and liver toxicity induced by diethyl maleate in rats

Abstract

The protective role of Tropaelum majus (T.majus) methyl alcohol extract and vitamin E in the case of toxic effect induced by diethyl maleate was evaluated. Forty-two male albino rats were divided into seven groups of six rats each for 15 days. Group 1: normal control group. Group 2: taken daily oral dose of paraffin oil (0.25ml/100g b.wt rat). Group 3: received daily oral dose of vitamin E (100mg/kg b.wt rat). Group 4: taken daily oral dose of 10% of the LD50 of T.majus methyl alcohol extract. Groups 5–7: injected intra-peritoneally with diethyl maleate (5 μl/100g b.wt rat) but groups 6 and 7 received a daily oral dose of either vitamin E or 10% of the LD50 of T.majus methyl alcohol extract 1h prior to diethyl maleate injection. The present results revealed that diethyl maleate induced serum aspartate and alanine aminotransferases enzymes activities decreased in serum, but their activities in the hepatic tissue showed an increase. Glutathione and glucose-6-phosphate dehydrogenase levels showed a decrease, but thiobarbituric acid reactive substances level showed an increase in both serum and liver tissue. Serum and liver proteins decreased in serum and liver tissue. A significant decrease in blood parameters (hemoglobin, hematocrit, as well as red and white blood cells) and serum glucose occurred. Histopathological results showed that diethyl maleate induced a hoop of edema in the hepatic periportal area; while T.majus methyl alcohol extract or vitamin E prior to diethyl maleate injection shift blood and liver toxicity induced by diethyl maleate towards normal values and preserved hepatic lobular architecture. In conclusion, pre-treatment with either T.majus methyl alcohol extract or vitamin E provide protection against blood and liver toxicity induced by diethyl maleate in rats, these results were confirmed by histological examinations.

Protective effect of Cupressus sempervirens extract against indomethacin-induced gastric ulcer in rats

“…The lethal dose fifity (LD 50 ) of C. sempervirens extract was determined previousely: 800 mg/kg bwt (Koriem, 2009 ), while 5%, 10% & 20% of the LD 50 of the plant were found to be safe (Koriem et al ., 2010 ). A pilot dose-response study included 15 gastric ucler rats pre-treated with 5%, 10% and 20% of the LD 50 of C. sempervirens leaves extract 1 h prior to indomethacin administration.…”

Therapeutic effect of Arctium lappa in Schistosoma haematobium associated kidney disturbance: biochemical and molecular effects

“…Mortality was assessed and counted in the different groups. The LD 50 was calculated according to Behrens and Karber (1953) and 1/10 of the LD 50 was found to be safe (Koriem et al 2010).…”

Anti-adipogenic effects of Tropaeolum majus (nasturtium) ethanol extract on 3T3-L1 cells

“…These flavonoids are known to have diuretic, anti-inflammatory, and anti-acidic effects, and they can inhibit lipid peroxide production and adipocyte differentiation [37–40], which is why we investigated anti-adipogenic properties of T. majus flowers. Furthermore, T. majus is rich in fatty acids and glucosinolates such as glucotropaeolin, which are heat-stable and possess strong anti-inflammatory and anti-cancer activities [41]. …”