Protective effect of Cupressus sempervirens extract against indomethacin-induced gastric ulcer in rats

Abstract

Cupressus sempervirens (C. sempervirens) belongs to the family Cupressaceae. It is widspread in Northern Africa, Greece, Turkey, North America, Cyprus and Syria. Cupressuflavone is the major ingredient of the plant leave extract. The aim of the present study was to evaluate the antiulcerogenic activity of the extract of C. sempervirens leaves in gastric ulcer tissues induced by indomethacin. The results of the present study revealed that indomethacin significantly decreased glutathione S-transferase (GST), glutathione peroxidase (GPx), catalase (CAT), reduced glutathione (GSH), glutathione reductase (GR) and superoxide dismutase (SOD) levels, while it increased significantly lipid peroxidation (MDA), nitric oxide (NO) and protein carbonyl (PC) levels in gastric tissue. Furthermore, indomethacin decreased p53 expression, while it increased bcl-2 expression in gastric tissue. Pretreatment with 5%, 10% & 20% of the LD50 of C. sempervirens and cupressuflavone of indomethacin-treated rats restored all the above parameters to approach normal values. C. sempervirens at the highest dose was more effective than the two lower doses. C. sempervirens proved more potent than cupressuflavone. In conclusion, C. sempervirens exerted antiulcerogenic activity and the effect was dose-dependent and related to the cupressuflavone ingredient of the plant leave extract.

IL-6 and NFE2L2: A putative role for the hepatoprotective effect of N. Sativa, P. Ginseng and C. Sempervirens in AFB-1 induced hepatocellular carcinoma in rats

“…Our data supports other studies that showed that health beneficial effects as antioxidant and anti-inflammatory effects can be achieved using plant–derived flavonoids [60,61]. This can be achieved by modulation of gene expression of certain proteins involved in the inflammatory response and alteration of activity levels of enzymes involved in antioxidant response [26].…”

“…Sempervirens extract administration protected against indomethacin-induced gastric ulcer. Such protection was associated with increased SOD activity [26].…”

“…Experimental doses of all herbs were selected based on previous studies and within non-toxic range [26,37,38]. Rats were randomly allocated into nine groups of six animals each. Group 1 (control): control group injected (i.p.)…”

“…C upressus Sempervirens (C. Sempervirens) commonly known as American yellow jasmine was reported to have potential anticancer effects both in vitro and in vivo [24,25]. It is a native herb in north Africa that possesses several therapeutic actions [26]. Its extract was shown to inhibit cancer cell proliferation in mouse skin carcinoma model [27].…”

Effects of Cupressus sempervirens extract on the healing of acetic acid-induced ulcerative colitis in rat

“…Koriem et al reported that cupressuflavone, a flavonoid found in C. sempervirens showed dose-dependent antiulcerogenic activity on gastric ulcer. 11 Other major components of this plant include amenoflavone, rutin, quercetin and myricitrin flavonoids which also show antioxidant activities. 12 In addition, the leaf extract of C. sempervirens is used for the treatment of gastrointestinal disorders 13 and stimulates accelerated action on slow-healing wounds.…”