The Protective Mechanism of Afuresertib against Esophageal Cancer

Abstract

Esophageal cancer (EC) is a common malignant tumor of the digestive system. Exploring the molecular biological mechanism of EC will help to clarify its carcinogenesis mechanism, find important molecular targets in the process of carcinogenesis, and provide new ideas for the diagnosis and treatment of EC. Phosphatidylinositol-3-kinase (PI3K)/protein kinase B (Akt) signaling pathway is one of the signal transduction pathways most closely related to cell proliferation and apoptosis. The regulation of various downstream molecules affects the proliferation and growth of tumor cells. In this study, we determined the effect of different concentrations of afuresertib on cell viability by MTT assay and determined the effect of afuresertib on cell apoptosis by Annexin V-FITC/PI dual staining. Animal experiments verified the effects of afuresertib on VEGF, bFGF, and PI3K/Akt. Our results indicated that afuresertib is closely related to the survival, proliferation, and apoptosis of esophageal cancer cell lines. More importantly, we found that afuresertib could reduce tumor volume and mass in EC rats through in vivo experiments. In conclusion, afuresertib may exert its antitumor effect by inhibiting the expression of PI3K and Akt-related proteins in rat tumor tissues.

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“…As a new inhibitor of AKT signal pathway, Afuresertib can be used to treat a variety of tumors. Studies in esophageal cancer have found that Afuresertib antagonizes tumor progression mainly by inhibiting the expression of PI3K and Akt pathway proteins [45].…”

Role of anoikis-related gene PLK1 in kidney renal papillary cell carcinoma: a bioinformatics analysis and preliminary verification on promoting proliferation and migration

“…Afuresertib is an Akt pathway inhibitor that exerts cytotoxic and antiproliferative activities in human cancer cells ( Uko et al, 2020 ). And in the study in rats, it was also found that Afuresertib can inhibit the expression of PI3K and Akt-related proteins in rat tumor tissues to exert its anti-tumor effect ( Min et al, 2022 ). These show that Afuresertib has anti-tumor effects in both humans and animals.…”