The protective effects of nitric oxide on the contralateral testis in prepubertal rats with unilateral testicular torsion

Dokucu, Ali İhsan; Öztürk, Hayrettin; Özdemir, Enver; Ketani, Aydın; Büyükbayram, Hüseyin; Yücesan, Selçuk

doi:10.1046/j.1464-410x.2000.00529.x

Cited by 34 publications

(22 citation statements)

References 36 publications

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“…10 Several studies have reported a protective effect of L-arginine (NO precursor) in ischemia-reperfusion (I/R) injury in rat testis torsion models. 11,12 Tadalafil is a phosphodiesterase-5 (PDE5) inhibitor mostly used for treatment of erectile dysfunction. 13 Inhibition of PDE5 enzymes may cause increased cGMP levels, ultimately resulting in smooth muscle relaxation.…”

Section: Introductionmentioning

confidence: 99%

“…Dokucu et al reported a protective effect of Arg on testicular histology of the controlateral testis following an ischemia period of six hours. 11 The authors evaluated testicular tissue 21 days after I/R and speculated NO was acting as a vasodilator to protect testis. Similarly, Barlas et al found Arg-infused rats had reduced reperfusion injury in a 30-minute torsion/detrosion model.…”

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confidence: 99%

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The protective effect of L-arginine, tadalafil, and their combination in rat testes after ischemia and reperfusion injury

Ozmerdiven

Çoşkun

Kaygısız

et al. 2017

CUAJ

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Cite as: Can Urol Assoc J 2017;11(1-2):E19-25. http://dx.doi.org/10.5489/cuaj.3872 Published online January 12, 2017 AbstractIntroduction: Nitric oxide (NO) plays an important role in the ischemia and reperfusion process. In this study, we aimed to examine the effect of L-arginine, tadalafil, and their combination for prevention of the ischemia reperfusion injury after testis torsion in rats. Methods: A total of 40 adult, male Sprague-Dawley rats were allocated into five groups. Three hours of left testicular torsion was performed in each group, excluding the control group. While the ischemia reperfusion (I/R) group had no treatment, I/R + Arg group received L-arginine, I/R + Td group received tadalafil and I/R + Arg + Td group received tadalafil and L-arginine 30 minutes before the detorsion. Then the left testis was untwisted for four hours of reperfusion. After bilateral orchiectomy, lipid peroxidation (LPx) and glutathione (GSH) activities were examined in testicular tissue. Spermatogenesis was evaluated with Johnsen's score. Results: LPx levels of the I/R group were found to be significantly higher than for groups that received drugs for both testes (p<0.001). GSH levels of the combination group were higher than I/R group in ipsilateral testis (p<0.01) and it was significantly higher than other groups for contralateral testis (p<0.001 for I/R group, p<0.01 for I/R + Arg, p<0.05 for I/R + Td). Mean Johnsen's score of the I/R group was found to be significantly lower than treatment groups in ipsilateral testis (p<0.001 for I/R + Arg + Td group, p<0.01 for other treatment goups) and contralateral testis (p<0.001). The mean Johnsen score of the combination group was significantly higher than that of other treatment groups in ipsilateral testis (p<0.05) and it was significantly higher than in the I/R + Td group in the contralateral testis (p<0.05). Conclusions: L-arginine, tadalafil, and combination of these two molecules showed protective effect against ischemia/reperfusion injury for both testes after unilateral testis torsion.

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Section: Introductionmentioning

confidence: 99%

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confidence: 99%

The protective effect of L-arginine, tadalafil, and their combination in rat testes after ischemia and reperfusion injury

Ozmerdiven

Çoşkun

Kaygısız

et al. 2017

CUAJ

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“…Oxygen free radicals oxidize membrane lipids, proteins, and DNA, leading to cellular dysfunction and cell death [5]. Some animal models of testicular torsion lipid peroxidation and the effect of nitric oxide (NO) have been measured [6,7].…”

Section: Introductionmentioning

confidence: 99%

The effects of molsidomine on hypoxia inducible factor alpha and Sonic hedgehog in testicular ischemia/reperfusion injury in rats

Dokucu

Öztürk

et al. 2008

Int Urol Nephrol

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This study was designed to determine the effect of molsidomine (MO), a precursor of nitric oxide (NO) donor, on hypoxia inducible factor alpha (HIF-1alpha) and Sonic hedgehog (Shh) levels considered to be involved in the development of testes ischemia/reperfusion (I-R) injury. Torsions were created by rotating ipsilateral testes 720 degrees in a clockwise direction for 6 h and 1-h detorsion of the testis was performed. A sham operation was performed in group 1 (control, n = 7). In group 2 (I-R/Untreated, n = 7), following 6 h of unilateral testicular torsion, 1-h detorsion of the testis was performed. No drug was given. In group 3 (I-R/MO), after performing the same surgical procedure as in group 2, a NO donor MO was given at the starting time of reperfusion. In group 4 (I-R/L-NAME), after performing the same surgical procedure as in group 2, L-NAME was given at the starting time of reperfusion. Testes malondialdehyde (MDA) levels were determined as well as examining the testes histologically. Treatment of rats with MO produced a significant reduction in the levels of MDA and histopathological score compared to testes I-R groups. The Sonic hedgehog (Shh) expression in the basement membrane of the tubuli seminiferi, and sertoli and germinal cells in testicular tissue, were greatly increased in the I-R/MO group compared to groups 1, 2 and 4. Additionally, the HIF-1alpha expression in the interstitial spaces in testicular tissue were greatly increased in the I-R/MO group. The results suggest that MO has a protective effect against ischemia/reperfusion injury in rat testes and may affect Shh and HIF-1alpha signaling pathway.

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“…The critical time for preventing contralateral testicular damage was reported to be 3 h [5,34] . Conversely, a 6-to 8-hour period of ischemia has been accepted as the minimum duration of ischemia before the contralateral testis is damaged [35] . However, in this research, we showed that 5 h of ischemia caused obvious damage in the contralateral testis.…”

Section: Discussionmentioning

confidence: 99%

Effect of Curcumin on Ipsilateral and Contralateral Testes after Unilateral Testicular Torsion in a Rat Model

Başaran¹,

Dökmeci²,

Yalçın³

et al. 2008

Urol Int

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Objective: The aim of the study was to determine the protective effect of curcumin on testicular ischemia-reperfusion (I/R) injury. Materials and Methods: 32 male rats were divided into four groups (n = 8): group 1: control; group 2: ischemia; group 3: I/R, and group 4: I/R+CUR. Curcumin (150 mg/kg, p.o.) was administered before 30 min of reperfusion in group 4. Malondialdehyde (MDA) levels, Johnsen’s testicular biopsy scores, and mean seminiferous tubule diameter measurements were evaluated in testes. In addition, endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS) expressions were evaluated immunohistochemically. Results: MDA levels in control groups were significantly lower than other groups in ipsilateral and contralateral testes. Johnsen’s scores in the control group were significantly higher than in other groups. MDA levels and Johnsen’s scores in the I/R+CUR group were similar to the ischemia and I/R groups in ipsilateral and contralateral testes. The immunoreactivity of iNOS and eNOS were increased in I/R ipsilateral testicular groups. After I/R, iNOS and eNOS expression increased slightly in contralateral groups. Additionally, the curcumin treatment decreased iNOS and eNOS immunoreactivity in ipsilateral and contralateral testes. Conclusion: The results suggest that curcumin did not protect the unilateral nor contralateral testes. This observation may depend on inhibition of iNOS and eNOS due to inhibition of the antioxidant, anti-inflammatory effects of nitric oxide.

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The protective effects of nitric oxide on the contralateral testis in prepubertal rats with unilateral testicular torsion

Cited by 34 publications

References 36 publications

The protective effect of L-arginine, tadalafil, and their combination in rat testes after ischemia and reperfusion injury

The protective effect of L-arginine, tadalafil, and their combination in rat testes after ischemia and reperfusion injury

The effects of molsidomine on hypoxia inducible factor alpha and Sonic hedgehog in testicular ischemia/reperfusion injury in rats

Effect of Curcumin on Ipsilateral and Contralateral Testes after Unilateral Testicular Torsion in a Rat Model

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